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A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs

Streptococcus suis (S. suis) and Pasteurella multocida (P. multocida) are pathogens that can cause zoonotic diseases. P. multocida toxin (PMT) is an important virulence factor that causes atrophic rhinitis in pigs. Suilysin (Sly) is an extracellular protein of S. suis and has been shown to be a pote...

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Autores principales: Wu, Min-Chia, Wu, Hsing-Chieh, Lee, Jai-Wei, Chang, Wan-Chen, Chu, Chun-Yen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9979465/
https://www.ncbi.nlm.nih.gov/pubmed/36864537
http://dx.doi.org/10.1186/s13567-023-01150-4
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author Wu, Min-Chia
Wu, Hsing-Chieh
Lee, Jai-Wei
Chang, Wan-Chen
Chu, Chun-Yen
author_facet Wu, Min-Chia
Wu, Hsing-Chieh
Lee, Jai-Wei
Chang, Wan-Chen
Chu, Chun-Yen
author_sort Wu, Min-Chia
collection PubMed
description Streptococcus suis (S. suis) and Pasteurella multocida (P. multocida) are pathogens that can cause zoonotic diseases. P. multocida toxin (PMT) is an important virulence factor that causes atrophic rhinitis in pigs. Suilysin (Sly) is an extracellular protein of S. suis and has been shown to be a potential adjuvant. Previous studies have indicated that subunit vaccines containing several fragments of PMT as antigens are safer than traditional inactivated or live-attenuated vaccines. However, protein-based vaccines need strong adjuvants to enhance their immunogenicity. In this study, recombinant PMT-NC (rPMT-NC) protein antigen was formulated with either recombinant Sly (rSly) or CpG oligodeoxynucleotides (CpG) as the adjuvant. The immune responses elicited by these vaccines and the protective efficacy after challenge with live P. multocida were evaluated in piglets. In the dose-dependent test, piglets immunized with the low dose (100 µg) of rSly had increased antigen-specific total IgG, interferon (IFN)-γ gene expression, and CD4(+) and CD8(+) T-cell populations. Compared to piglets in the commercial (Al-gel) adjuvant and the control groups (p < 0.05), piglets in the biological adjuvant groups showed significantly reduced turbinate atrophy, nasal distortion, and lung lesion scores after challenge with P. multocida serotype A. Vaccines containing rSly or CpG adjuvant enhanced humoral and cellular immune responses and protection against P. multocida. This combination of a protein-based antigen formulated with a biological adjuvant showed synergistic and protective effects against atrophic rhinitis and has potential to be developed as part of a bivalent vaccine.
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spelling pubmed-99794652023-03-03 A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs Wu, Min-Chia Wu, Hsing-Chieh Lee, Jai-Wei Chang, Wan-Chen Chu, Chun-Yen Vet Res Research Article Streptococcus suis (S. suis) and Pasteurella multocida (P. multocida) are pathogens that can cause zoonotic diseases. P. multocida toxin (PMT) is an important virulence factor that causes atrophic rhinitis in pigs. Suilysin (Sly) is an extracellular protein of S. suis and has been shown to be a potential adjuvant. Previous studies have indicated that subunit vaccines containing several fragments of PMT as antigens are safer than traditional inactivated or live-attenuated vaccines. However, protein-based vaccines need strong adjuvants to enhance their immunogenicity. In this study, recombinant PMT-NC (rPMT-NC) protein antigen was formulated with either recombinant Sly (rSly) or CpG oligodeoxynucleotides (CpG) as the adjuvant. The immune responses elicited by these vaccines and the protective efficacy after challenge with live P. multocida were evaluated in piglets. In the dose-dependent test, piglets immunized with the low dose (100 µg) of rSly had increased antigen-specific total IgG, interferon (IFN)-γ gene expression, and CD4(+) and CD8(+) T-cell populations. Compared to piglets in the commercial (Al-gel) adjuvant and the control groups (p < 0.05), piglets in the biological adjuvant groups showed significantly reduced turbinate atrophy, nasal distortion, and lung lesion scores after challenge with P. multocida serotype A. Vaccines containing rSly or CpG adjuvant enhanced humoral and cellular immune responses and protection against P. multocida. This combination of a protein-based antigen formulated with a biological adjuvant showed synergistic and protective effects against atrophic rhinitis and has potential to be developed as part of a bivalent vaccine. BioMed Central 2023-03-02 2023 /pmc/articles/PMC9979465/ /pubmed/36864537 http://dx.doi.org/10.1186/s13567-023-01150-4 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research Article
Wu, Min-Chia
Wu, Hsing-Chieh
Lee, Jai-Wei
Chang, Wan-Chen
Chu, Chun-Yen
A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs
title A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs
title_full A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs
title_fullStr A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs
title_full_unstemmed A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs
title_short A protein-based subunit vaccine with biological adjuvants provides effective protection against Pasteurella multocida in pigs
title_sort protein-based subunit vaccine with biological adjuvants provides effective protection against pasteurella multocida in pigs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9979465/
https://www.ncbi.nlm.nih.gov/pubmed/36864537
http://dx.doi.org/10.1186/s13567-023-01150-4
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