Cargando…

Stress-induced immunosuppression affecting immune response to Newcastle disease virus vaccine through “miR-155-CTLA-4” pathway in chickens

MiR-155 and CTLA-4 are important factors involved in the regulation of immune function. However, there is no report about their involvement in function regulation of stress-induced immunosuppression affecting immune response. In this study, the chicken model of stress-induced immunosuppression affec...

Descripción completa

Detalles Bibliográficos
Autores principales: Wen, Jie, Wu, Yiru, Han, Jianwei, Tian, Yufei, Man, Chaolai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9979835/
https://www.ncbi.nlm.nih.gov/pubmed/36874964
http://dx.doi.org/10.7717/peerj.14529
_version_ 1784899798835396608
author Wen, Jie
Wu, Yiru
Han, Jianwei
Tian, Yufei
Man, Chaolai
author_facet Wen, Jie
Wu, Yiru
Han, Jianwei
Tian, Yufei
Man, Chaolai
author_sort Wen, Jie
collection PubMed
description MiR-155 and CTLA-4 are important factors involved in the regulation of immune function. However, there is no report about their involvement in function regulation of stress-induced immunosuppression affecting immune response. In this study, the chicken model of stress-induced immunosuppression affecting immune response (simulation with dexamethasone and immunization with Newcastle disease virus (NDV) attenuated vaccine) was established, then the expression characteristics of miR-155 and CTLA-4 gene were analyzed at several key time points during the processes of stress-induced immunosuppression affecting NDV vaccine immune response at serum and tissue levels. The results showed that miR-155 and CTLA-4 were the key factors involved in stress-induced immunosuppression and NDV immune response, whose functions involved in the regulation of immune function were different in different tissues and time points, and 2 day post immunization (dpi), 5dpi and 21dpi were the possible key regulatory time points. CTLA-4, the target gene of miR-155, had significant game regulation relationships between them in various tissues, such as bursa of Fabricius, thymus and liver, indicating that miR-155-CTLA-4 pathway was one of the main mechanisms of their involvement in the regulations of stress-induced immunosuppression affecting NDV immune response. This study can lay the foundation for in-depth exploration of miR-155-CTLA-4 pathway involved in the regulation of immune function.
format Online
Article
Text
id pubmed-9979835
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher PeerJ Inc.
record_format MEDLINE/PubMed
spelling pubmed-99798352023-03-03 Stress-induced immunosuppression affecting immune response to Newcastle disease virus vaccine through “miR-155-CTLA-4” pathway in chickens Wen, Jie Wu, Yiru Han, Jianwei Tian, Yufei Man, Chaolai PeerJ Agricultural Science MiR-155 and CTLA-4 are important factors involved in the regulation of immune function. However, there is no report about their involvement in function regulation of stress-induced immunosuppression affecting immune response. In this study, the chicken model of stress-induced immunosuppression affecting immune response (simulation with dexamethasone and immunization with Newcastle disease virus (NDV) attenuated vaccine) was established, then the expression characteristics of miR-155 and CTLA-4 gene were analyzed at several key time points during the processes of stress-induced immunosuppression affecting NDV vaccine immune response at serum and tissue levels. The results showed that miR-155 and CTLA-4 were the key factors involved in stress-induced immunosuppression and NDV immune response, whose functions involved in the regulation of immune function were different in different tissues and time points, and 2 day post immunization (dpi), 5dpi and 21dpi were the possible key regulatory time points. CTLA-4, the target gene of miR-155, had significant game regulation relationships between them in various tissues, such as bursa of Fabricius, thymus and liver, indicating that miR-155-CTLA-4 pathway was one of the main mechanisms of their involvement in the regulations of stress-induced immunosuppression affecting NDV immune response. This study can lay the foundation for in-depth exploration of miR-155-CTLA-4 pathway involved in the regulation of immune function. PeerJ Inc. 2023-02-27 /pmc/articles/PMC9979835/ /pubmed/36874964 http://dx.doi.org/10.7717/peerj.14529 Text en ©2023 Wen et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Agricultural Science
Wen, Jie
Wu, Yiru
Han, Jianwei
Tian, Yufei
Man, Chaolai
Stress-induced immunosuppression affecting immune response to Newcastle disease virus vaccine through “miR-155-CTLA-4” pathway in chickens
title Stress-induced immunosuppression affecting immune response to Newcastle disease virus vaccine through “miR-155-CTLA-4” pathway in chickens
title_full Stress-induced immunosuppression affecting immune response to Newcastle disease virus vaccine through “miR-155-CTLA-4” pathway in chickens
title_fullStr Stress-induced immunosuppression affecting immune response to Newcastle disease virus vaccine through “miR-155-CTLA-4” pathway in chickens
title_full_unstemmed Stress-induced immunosuppression affecting immune response to Newcastle disease virus vaccine through “miR-155-CTLA-4” pathway in chickens
title_short Stress-induced immunosuppression affecting immune response to Newcastle disease virus vaccine through “miR-155-CTLA-4” pathway in chickens
title_sort stress-induced immunosuppression affecting immune response to newcastle disease virus vaccine through “mir-155-ctla-4” pathway in chickens
topic Agricultural Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9979835/
https://www.ncbi.nlm.nih.gov/pubmed/36874964
http://dx.doi.org/10.7717/peerj.14529
work_keys_str_mv AT wenjie stressinducedimmunosuppressionaffectingimmuneresponsetonewcastlediseasevirusvaccinethroughmir155ctla4pathwayinchickens
AT wuyiru stressinducedimmunosuppressionaffectingimmuneresponsetonewcastlediseasevirusvaccinethroughmir155ctla4pathwayinchickens
AT hanjianwei stressinducedimmunosuppressionaffectingimmuneresponsetonewcastlediseasevirusvaccinethroughmir155ctla4pathwayinchickens
AT tianyufei stressinducedimmunosuppressionaffectingimmuneresponsetonewcastlediseasevirusvaccinethroughmir155ctla4pathwayinchickens
AT manchaolai stressinducedimmunosuppressionaffectingimmuneresponsetonewcastlediseasevirusvaccinethroughmir155ctla4pathwayinchickens