WTAP regulates autophagy in colon cancer cells by inhibiting FLNA through N6-methyladenosine

Our study investigated the role of WTAP in colon cancer. We employed experiments including m6A dot blot hybridization, methylated RNA immunoprecipitation, dual-luciferase, and RNA immunoprecipitation to investigate the regulatory mechanism of WTAP. Western blot was performed to analyze the expressio...

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Detalles Bibliográficos
Autores principales: Huang, Liang, Shao, Jinfan, Xu, Xijuan, Hong, Weiwen, Yu, Wenfeng, Zheng, Shuang, Ge, Xiaogang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9980444/
https://www.ncbi.nlm.nih.gov/pubmed/36849408
http://dx.doi.org/10.1080/19336918.2023.2180196
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author Huang, Liang
Shao, Jinfan
Xu, Xijuan
Hong, Weiwen
Yu, Wenfeng
Zheng, Shuang
Ge, Xiaogang
author_facet Huang, Liang
Shao, Jinfan
Xu, Xijuan
Hong, Weiwen
Yu, Wenfeng
Zheng, Shuang
Ge, Xiaogang
author_sort Huang, Liang
collection PubMed
description Our study investigated the role of WTAP in colon cancer. We employed experiments including m6A dot blot hybridization, methylated RNA immunoprecipitation, dual-luciferase, and RNA immunoprecipitation to investigate the regulatory mechanism of WTAP. Western blot was performed to analyze the expression of WTAP, FLNA and autophagy-related proteins in cells. Our results confirmed the up-regulation of WTAP in colon cancer and its promoting effect on proliferation and inhibiting effect on apoptosis. FLNA was the downstream gene of WTAP and WTAP-regulated m6A modification led to post-transcriptional repression of FLNA. The rescue experiments showed that WTAP/FLNA could inhibit autophagy. WTAP-mediated m6A modification was confirmed to be crucial in colon cancer development, providing new insights into colon cancer therapy.
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spelling pubmed-99804442023-03-03 WTAP regulates autophagy in colon cancer cells by inhibiting FLNA through N6-methyladenosine Huang, Liang Shao, Jinfan Xu, Xijuan Hong, Weiwen Yu, Wenfeng Zheng, Shuang Ge, Xiaogang Cell Adh Migr Research Paper Our study investigated the role of WTAP in colon cancer. We employed experiments including m6A dot blot hybridization, methylated RNA immunoprecipitation, dual-luciferase, and RNA immunoprecipitation to investigate the regulatory mechanism of WTAP. Western blot was performed to analyze the expression of WTAP, FLNA and autophagy-related proteins in cells. Our results confirmed the up-regulation of WTAP in colon cancer and its promoting effect on proliferation and inhibiting effect on apoptosis. FLNA was the downstream gene of WTAP and WTAP-regulated m6A modification led to post-transcriptional repression of FLNA. The rescue experiments showed that WTAP/FLNA could inhibit autophagy. WTAP-mediated m6A modification was confirmed to be crucial in colon cancer development, providing new insights into colon cancer therapy. Taylor & Francis 2023-02-27 /pmc/articles/PMC9980444/ /pubmed/36849408 http://dx.doi.org/10.1080/19336918.2023.2180196 Text en © 2023 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Huang, Liang
Shao, Jinfan
Xu, Xijuan
Hong, Weiwen
Yu, Wenfeng
Zheng, Shuang
Ge, Xiaogang
WTAP regulates autophagy in colon cancer cells by inhibiting FLNA through N6-methyladenosine
title WTAP regulates autophagy in colon cancer cells by inhibiting FLNA through N6-methyladenosine
title_full WTAP regulates autophagy in colon cancer cells by inhibiting FLNA through N6-methyladenosine
title_fullStr WTAP regulates autophagy in colon cancer cells by inhibiting FLNA through N6-methyladenosine
title_full_unstemmed WTAP regulates autophagy in colon cancer cells by inhibiting FLNA through N6-methyladenosine
title_short WTAP regulates autophagy in colon cancer cells by inhibiting FLNA through N6-methyladenosine
title_sort wtap regulates autophagy in colon cancer cells by inhibiting flna through n6-methyladenosine
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9980444/
https://www.ncbi.nlm.nih.gov/pubmed/36849408
http://dx.doi.org/10.1080/19336918.2023.2180196
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