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Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity

Bacterial colonization in the gut plays a pivotal role in neonatal necrotizing enterocolitis (NEC) development, but the relationship between bacteria and NEC remains unclear. In this study, we aimed to elucidate whether bacterial butyrate end-fermentation metabolites participate in the development o...

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Autores principales: Ferraris, Laurent, Balvay, Aurélie, Bellet, Deborah, Delannoy, Johanne, Maudet, Claire, Larcher, Thibaut, Rozé, Jean-Christophe, Philippe, Catherine, Meylheuc, Thierry, Butel, Marie-José, Rabot, Sylvie, Aires, Julio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9980464/
https://www.ncbi.nlm.nih.gov/pubmed/36801067
http://dx.doi.org/10.1080/19490976.2023.2172666
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author Ferraris, Laurent
Balvay, Aurélie
Bellet, Deborah
Delannoy, Johanne
Maudet, Claire
Larcher, Thibaut
Rozé, Jean-Christophe
Philippe, Catherine
Meylheuc, Thierry
Butel, Marie-José
Rabot, Sylvie
Aires, Julio
author_facet Ferraris, Laurent
Balvay, Aurélie
Bellet, Deborah
Delannoy, Johanne
Maudet, Claire
Larcher, Thibaut
Rozé, Jean-Christophe
Philippe, Catherine
Meylheuc, Thierry
Butel, Marie-José
Rabot, Sylvie
Aires, Julio
author_sort Ferraris, Laurent
collection PubMed
description Bacterial colonization in the gut plays a pivotal role in neonatal necrotizing enterocolitis (NEC) development, but the relationship between bacteria and NEC remains unclear. In this study, we aimed to elucidate whether bacterial butyrate end-fermentation metabolites participate in the development of NEC lesions and confirm the enteropathogenicity of Clostridium butyricum and Clostridium neonatale in NEC. First, we produced C.butyricum and C.neonatale strains impaired in butyrate production by genetically inactivating the hbd gene encoding β-hydroxybutyryl-CoA dehydrogenase that produces end-fermentation metabolites. Second, we evaluated the enteropathogenicty of the hbd-knockout strains in a gnotobiotic quail model of NEC. The analyses showed that animals harboring these strains had significantly fewer and less intense intestinal lesions than those harboring the respective wild-type strains. In the absence of specific biological markers of NEC, the data provide original and new mechanistic insights into the disease pathophysiology, a necessary step for developing potential novel therapies.
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spelling pubmed-99804642023-03-03 Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity Ferraris, Laurent Balvay, Aurélie Bellet, Deborah Delannoy, Johanne Maudet, Claire Larcher, Thibaut Rozé, Jean-Christophe Philippe, Catherine Meylheuc, Thierry Butel, Marie-José Rabot, Sylvie Aires, Julio Gut Microbes Research Paper Bacterial colonization in the gut plays a pivotal role in neonatal necrotizing enterocolitis (NEC) development, but the relationship between bacteria and NEC remains unclear. In this study, we aimed to elucidate whether bacterial butyrate end-fermentation metabolites participate in the development of NEC lesions and confirm the enteropathogenicity of Clostridium butyricum and Clostridium neonatale in NEC. First, we produced C.butyricum and C.neonatale strains impaired in butyrate production by genetically inactivating the hbd gene encoding β-hydroxybutyryl-CoA dehydrogenase that produces end-fermentation metabolites. Second, we evaluated the enteropathogenicty of the hbd-knockout strains in a gnotobiotic quail model of NEC. The analyses showed that animals harboring these strains had significantly fewer and less intense intestinal lesions than those harboring the respective wild-type strains. In the absence of specific biological markers of NEC, the data provide original and new mechanistic insights into the disease pathophysiology, a necessary step for developing potential novel therapies. Taylor & Francis 2023-02-21 /pmc/articles/PMC9980464/ /pubmed/36801067 http://dx.doi.org/10.1080/19490976.2023.2172666 Text en © 2023 The Author(s). Published with license by Taylor & Francis Group, LLC. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Ferraris, Laurent
Balvay, Aurélie
Bellet, Deborah
Delannoy, Johanne
Maudet, Claire
Larcher, Thibaut
Rozé, Jean-Christophe
Philippe, Catherine
Meylheuc, Thierry
Butel, Marie-José
Rabot, Sylvie
Aires, Julio
Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity
title Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity
title_full Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity
title_fullStr Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity
title_full_unstemmed Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity
title_short Neonatal necrotizing enterocolitis: Clostridium butyricum and Clostridium neonatale fermentation metabolism and enteropathogenicity
title_sort neonatal necrotizing enterocolitis: clostridium butyricum and clostridium neonatale fermentation metabolism and enteropathogenicity
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9980464/
https://www.ncbi.nlm.nih.gov/pubmed/36801067
http://dx.doi.org/10.1080/19490976.2023.2172666
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