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System network analysis of Rosmarinus officinalis transcriptome and metabolome—Key genes in biosynthesis of secondary metabolites

Medicinal plants contain valuable compounds that have attracted worldwide interest for their use in the production of natural drugs. The presence of compounds such as rosmarinic acid, carnosic acid, and carnosol in Rosmarinus officinalis has made it a plant with unique therapeutic effects. The ident...

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Autores principales: Moghadam, Ali, Foroozan, Eisa, Tahmasebi, Ahmad, Taghizadeh, Mohammad Sadegh, Bolhassani, Mohammad, Jafari, Morteza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9980811/
https://www.ncbi.nlm.nih.gov/pubmed/36862714
http://dx.doi.org/10.1371/journal.pone.0282316
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author Moghadam, Ali
Foroozan, Eisa
Tahmasebi, Ahmad
Taghizadeh, Mohammad Sadegh
Bolhassani, Mohammad
Jafari, Morteza
author_facet Moghadam, Ali
Foroozan, Eisa
Tahmasebi, Ahmad
Taghizadeh, Mohammad Sadegh
Bolhassani, Mohammad
Jafari, Morteza
author_sort Moghadam, Ali
collection PubMed
description Medicinal plants contain valuable compounds that have attracted worldwide interest for their use in the production of natural drugs. The presence of compounds such as rosmarinic acid, carnosic acid, and carnosol in Rosmarinus officinalis has made it a plant with unique therapeutic effects. The identification and regulation of the biosynthetic pathways and genes will enable the large-scale production of these compounds. Hence, we studied the correlation between the genes involved in biosynthesis of the secondary metabolites in R. officinalis using proteomics and metabolomics data by WGCNA. We identified three modules as having the highest potential for the metabolite engineering. Moreover, the hub genes highly connected to particular modules, TFs, PKs, and transporters were identified. The TFs of MYB, C3H, HB, and C2H2 were the most likely candidates associated with the target metabolic pathways. The results indicated that the hub genes including Copalyl diphosphate synthase (CDS), Phenylalanine ammonia lyase (PAL), Cineole synthase (CIN), Rosmarinic acid synthase (RAS), Tyrosine aminotransferase (TAT), Cinnamate 4-hydroxylase (C4H), and MYB58 are responsible for biosynthesis of important secondary metabolites. Thus, we confirmed these results using qRT-PCR after treating R. officinalis seedlings with methyl jasmonate. These candidate genes may be employed for genetic and metabolic engineering research to increase R. officinalis metabolite production.
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spelling pubmed-99808112023-03-03 System network analysis of Rosmarinus officinalis transcriptome and metabolome—Key genes in biosynthesis of secondary metabolites Moghadam, Ali Foroozan, Eisa Tahmasebi, Ahmad Taghizadeh, Mohammad Sadegh Bolhassani, Mohammad Jafari, Morteza PLoS One Research Article Medicinal plants contain valuable compounds that have attracted worldwide interest for their use in the production of natural drugs. The presence of compounds such as rosmarinic acid, carnosic acid, and carnosol in Rosmarinus officinalis has made it a plant with unique therapeutic effects. The identification and regulation of the biosynthetic pathways and genes will enable the large-scale production of these compounds. Hence, we studied the correlation between the genes involved in biosynthesis of the secondary metabolites in R. officinalis using proteomics and metabolomics data by WGCNA. We identified three modules as having the highest potential for the metabolite engineering. Moreover, the hub genes highly connected to particular modules, TFs, PKs, and transporters were identified. The TFs of MYB, C3H, HB, and C2H2 were the most likely candidates associated with the target metabolic pathways. The results indicated that the hub genes including Copalyl diphosphate synthase (CDS), Phenylalanine ammonia lyase (PAL), Cineole synthase (CIN), Rosmarinic acid synthase (RAS), Tyrosine aminotransferase (TAT), Cinnamate 4-hydroxylase (C4H), and MYB58 are responsible for biosynthesis of important secondary metabolites. Thus, we confirmed these results using qRT-PCR after treating R. officinalis seedlings with methyl jasmonate. These candidate genes may be employed for genetic and metabolic engineering research to increase R. officinalis metabolite production. Public Library of Science 2023-03-02 /pmc/articles/PMC9980811/ /pubmed/36862714 http://dx.doi.org/10.1371/journal.pone.0282316 Text en © 2023 Moghadam et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Moghadam, Ali
Foroozan, Eisa
Tahmasebi, Ahmad
Taghizadeh, Mohammad Sadegh
Bolhassani, Mohammad
Jafari, Morteza
System network analysis of Rosmarinus officinalis transcriptome and metabolome—Key genes in biosynthesis of secondary metabolites
title System network analysis of Rosmarinus officinalis transcriptome and metabolome—Key genes in biosynthesis of secondary metabolites
title_full System network analysis of Rosmarinus officinalis transcriptome and metabolome—Key genes in biosynthesis of secondary metabolites
title_fullStr System network analysis of Rosmarinus officinalis transcriptome and metabolome—Key genes in biosynthesis of secondary metabolites
title_full_unstemmed System network analysis of Rosmarinus officinalis transcriptome and metabolome—Key genes in biosynthesis of secondary metabolites
title_short System network analysis of Rosmarinus officinalis transcriptome and metabolome—Key genes in biosynthesis of secondary metabolites
title_sort system network analysis of rosmarinus officinalis transcriptome and metabolome—key genes in biosynthesis of secondary metabolites
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9980811/
https://www.ncbi.nlm.nih.gov/pubmed/36862714
http://dx.doi.org/10.1371/journal.pone.0282316
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