Cargando…

GINS2 Promotes Osteosarcoma Tumorigenesis via STAT3/MYC Axis

GINS2 is overexpressed in several cancers, but little is known about its role in osteosarcoma (OS). A series of in vivo and in vitro experiments were conducted to explore the role of GINS2 in OS. In this study, we demonstrated that GINS2 was found to be highly expressed in OS tissues and cell lines,...

Descripción completa

Detalles Bibliográficos
Autores principales: Ren, Bingkai, Zheng, Yibin, Nie, Lizhong, Wu, Fanhui, Huang, Leiwen, Wei, Jingdu, Yang, Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981285/
https://www.ncbi.nlm.nih.gov/pubmed/36873736
http://dx.doi.org/10.1155/2023/8454142
_version_ 1784900068771364864
author Ren, Bingkai
Zheng, Yibin
Nie, Lizhong
Wu, Fanhui
Huang, Leiwen
Wei, Jingdu
Yang, Dong
author_facet Ren, Bingkai
Zheng, Yibin
Nie, Lizhong
Wu, Fanhui
Huang, Leiwen
Wei, Jingdu
Yang, Dong
author_sort Ren, Bingkai
collection PubMed
description GINS2 is overexpressed in several cancers, but little is known about its role in osteosarcoma (OS). A series of in vivo and in vitro experiments were conducted to explore the role of GINS2 in OS. In this study, we demonstrated that GINS2 was found to be highly expressed in OS tissues and cell lines, which was associated with poor outcomes in OS patients. GINS2 knockdown hindered the growth and induced apoptosis in OS cell lines in vitro. Furthermore, GINS2 knockdown effectively inhibited the growth of a xenograft tumor in vivo. By using an Affymetrix gene chip and intelligent pathway analysis, it was demonstrated that the GINS2 knockdown could reduce the expression of several targeted genes and reduce the activity of the MYC signaling pathway. Mechanically, LC-MS, CoIP, and rescue experiments revealed that GINS2 promoted tumor progression through the STAT3/MYC axis in the OS. Moreover, GINS2 was associated with tumor immunity and may be a potential immunotherapeutic target for OS.
format Online
Article
Text
id pubmed-9981285
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-99812852023-03-03 GINS2 Promotes Osteosarcoma Tumorigenesis via STAT3/MYC Axis Ren, Bingkai Zheng, Yibin Nie, Lizhong Wu, Fanhui Huang, Leiwen Wei, Jingdu Yang, Dong J Oncol Research Article GINS2 is overexpressed in several cancers, but little is known about its role in osteosarcoma (OS). A series of in vivo and in vitro experiments were conducted to explore the role of GINS2 in OS. In this study, we demonstrated that GINS2 was found to be highly expressed in OS tissues and cell lines, which was associated with poor outcomes in OS patients. GINS2 knockdown hindered the growth and induced apoptosis in OS cell lines in vitro. Furthermore, GINS2 knockdown effectively inhibited the growth of a xenograft tumor in vivo. By using an Affymetrix gene chip and intelligent pathway analysis, it was demonstrated that the GINS2 knockdown could reduce the expression of several targeted genes and reduce the activity of the MYC signaling pathway. Mechanically, LC-MS, CoIP, and rescue experiments revealed that GINS2 promoted tumor progression through the STAT3/MYC axis in the OS. Moreover, GINS2 was associated with tumor immunity and may be a potential immunotherapeutic target for OS. Hindawi 2023-02-23 /pmc/articles/PMC9981285/ /pubmed/36873736 http://dx.doi.org/10.1155/2023/8454142 Text en Copyright © 2023 Bingkai Ren et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ren, Bingkai
Zheng, Yibin
Nie, Lizhong
Wu, Fanhui
Huang, Leiwen
Wei, Jingdu
Yang, Dong
GINS2 Promotes Osteosarcoma Tumorigenesis via STAT3/MYC Axis
title GINS2 Promotes Osteosarcoma Tumorigenesis via STAT3/MYC Axis
title_full GINS2 Promotes Osteosarcoma Tumorigenesis via STAT3/MYC Axis
title_fullStr GINS2 Promotes Osteosarcoma Tumorigenesis via STAT3/MYC Axis
title_full_unstemmed GINS2 Promotes Osteosarcoma Tumorigenesis via STAT3/MYC Axis
title_short GINS2 Promotes Osteosarcoma Tumorigenesis via STAT3/MYC Axis
title_sort gins2 promotes osteosarcoma tumorigenesis via stat3/myc axis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981285/
https://www.ncbi.nlm.nih.gov/pubmed/36873736
http://dx.doi.org/10.1155/2023/8454142
work_keys_str_mv AT renbingkai gins2promotesosteosarcomatumorigenesisviastat3mycaxis
AT zhengyibin gins2promotesosteosarcomatumorigenesisviastat3mycaxis
AT nielizhong gins2promotesosteosarcomatumorigenesisviastat3mycaxis
AT wufanhui gins2promotesosteosarcomatumorigenesisviastat3mycaxis
AT huangleiwen gins2promotesosteosarcomatumorigenesisviastat3mycaxis
AT weijingdu gins2promotesosteosarcomatumorigenesisviastat3mycaxis
AT yangdong gins2promotesosteosarcomatumorigenesisviastat3mycaxis