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Circ_0110498 facilitates the cisplatin resistance of non‐small cell lung cancer by mediating the miR‐1287‐5p/RBBP4 axis
BACKGROUND: Circular RNAs (circRNAs) play vital roles in non‐small cell lung cancer (NSCLC) progression. Our research analyzed the role of circ_0110498 on the cisplatin (DDP) resistance of NSCLC. METHODS: Cell glycolysis was analyzed by measuring glucose consumption and lactate production. Protein e...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons Australia, Ltd
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981309/ https://www.ncbi.nlm.nih.gov/pubmed/36691322 http://dx.doi.org/10.1111/1759-7714.14787 |
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author | Hao, Dexun Li, Yanshuang Shi, Jiang Jiang, Junguang |
author_facet | Hao, Dexun Li, Yanshuang Shi, Jiang Jiang, Junguang |
author_sort | Hao, Dexun |
collection | PubMed |
description | BACKGROUND: Circular RNAs (circRNAs) play vital roles in non‐small cell lung cancer (NSCLC) progression. Our research analyzed the role of circ_0110498 on the cisplatin (DDP) resistance of NSCLC. METHODS: Cell glycolysis was analyzed by measuring glucose consumption and lactate production. Protein expression was determined by western blot analysis. The expression of circ_0110498, microRNA (miR)‐1287‐5p and RBBP4 was detected by RT‐qPCR assay. Cell counting kit‐8, colony formation and transwell assays, together with flow cytometry were conducted to analyze cell DDP resistance, proliferation, metastasis and apoptosis. RESULTS: Circ_0110498 expression was elevated in DDP‐resistant NSCLC tissues and cells. Circ_0110498 silencing not only suppressed the DDP resistance of NSCLC cells by inhibiting cell growth, metastasis and glycolysis, but also enhanced the DDP sensitivity of NSCLC tumors. MiR‐1287‐5p was sponged by circ_0110498, and its inhibitor also reversed the effect of circ_0110498 silencing on the DDP resistance of NSCLC cells. MiR‐1287‐5p interacted with RBBP4, and RBBP4 overexpression partly reversed the inhibitory effect of miR‐1287‐5p on the DDP resistance of NSCLC cells. CONCLUSION: Circ_0110498 facilitated DDP resistance partly through mediating the miR‐1287‐5p/RBBP4 signaling in NSCLC. |
format | Online Article Text |
id | pubmed-9981309 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley & Sons Australia, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-99813092023-03-03 Circ_0110498 facilitates the cisplatin resistance of non‐small cell lung cancer by mediating the miR‐1287‐5p/RBBP4 axis Hao, Dexun Li, Yanshuang Shi, Jiang Jiang, Junguang Thorac Cancer Original Articles BACKGROUND: Circular RNAs (circRNAs) play vital roles in non‐small cell lung cancer (NSCLC) progression. Our research analyzed the role of circ_0110498 on the cisplatin (DDP) resistance of NSCLC. METHODS: Cell glycolysis was analyzed by measuring glucose consumption and lactate production. Protein expression was determined by western blot analysis. The expression of circ_0110498, microRNA (miR)‐1287‐5p and RBBP4 was detected by RT‐qPCR assay. Cell counting kit‐8, colony formation and transwell assays, together with flow cytometry were conducted to analyze cell DDP resistance, proliferation, metastasis and apoptosis. RESULTS: Circ_0110498 expression was elevated in DDP‐resistant NSCLC tissues and cells. Circ_0110498 silencing not only suppressed the DDP resistance of NSCLC cells by inhibiting cell growth, metastasis and glycolysis, but also enhanced the DDP sensitivity of NSCLC tumors. MiR‐1287‐5p was sponged by circ_0110498, and its inhibitor also reversed the effect of circ_0110498 silencing on the DDP resistance of NSCLC cells. MiR‐1287‐5p interacted with RBBP4, and RBBP4 overexpression partly reversed the inhibitory effect of miR‐1287‐5p on the DDP resistance of NSCLC cells. CONCLUSION: Circ_0110498 facilitated DDP resistance partly through mediating the miR‐1287‐5p/RBBP4 signaling in NSCLC. John Wiley & Sons Australia, Ltd 2023-01-23 /pmc/articles/PMC9981309/ /pubmed/36691322 http://dx.doi.org/10.1111/1759-7714.14787 Text en © 2023 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Original Articles Hao, Dexun Li, Yanshuang Shi, Jiang Jiang, Junguang Circ_0110498 facilitates the cisplatin resistance of non‐small cell lung cancer by mediating the miR‐1287‐5p/RBBP4 axis |
title | Circ_0110498 facilitates the cisplatin resistance of non‐small cell lung cancer by mediating the miR‐1287‐5p/RBBP4 axis |
title_full | Circ_0110498 facilitates the cisplatin resistance of non‐small cell lung cancer by mediating the miR‐1287‐5p/RBBP4 axis |
title_fullStr | Circ_0110498 facilitates the cisplatin resistance of non‐small cell lung cancer by mediating the miR‐1287‐5p/RBBP4 axis |
title_full_unstemmed | Circ_0110498 facilitates the cisplatin resistance of non‐small cell lung cancer by mediating the miR‐1287‐5p/RBBP4 axis |
title_short | Circ_0110498 facilitates the cisplatin resistance of non‐small cell lung cancer by mediating the miR‐1287‐5p/RBBP4 axis |
title_sort | circ_0110498 facilitates the cisplatin resistance of non‐small cell lung cancer by mediating the mir‐1287‐5p/rbbp4 axis |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981309/ https://www.ncbi.nlm.nih.gov/pubmed/36691322 http://dx.doi.org/10.1111/1759-7714.14787 |
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