Cargando…

Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania

Thymus comosus Heuff ex. Griseb. (Lamiaceae) is a wild thyme species endemic for Romanian Carpathian areas, frequently collected as substitute for collective herbal product Serpylli herba, cited as antibacterial and diuretic remedy in traditional medicine. The present study aimed to evaluate the in...

Descripción completa

Detalles Bibliográficos
Autores principales: Babotă, Mihai, Frumuzachi, Oleg, Nicolescu, Alexandru, Stojković, Dejan, Soković, Marina, Rocchetti, Gabriele, Zhang, Leilei, Lucini, Luigi, Crișan, Gianina, Mocan, Andrei, Voștinaru, Oliviu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981668/
https://www.ncbi.nlm.nih.gov/pubmed/36874013
http://dx.doi.org/10.3389/fphar.2023.1115117
_version_ 1784900157749329920
author Babotă, Mihai
Frumuzachi, Oleg
Nicolescu, Alexandru
Stojković, Dejan
Soković, Marina
Rocchetti, Gabriele
Zhang, Leilei
Lucini, Luigi
Crișan, Gianina
Mocan, Andrei
Voștinaru, Oliviu
author_facet Babotă, Mihai
Frumuzachi, Oleg
Nicolescu, Alexandru
Stojković, Dejan
Soković, Marina
Rocchetti, Gabriele
Zhang, Leilei
Lucini, Luigi
Crișan, Gianina
Mocan, Andrei
Voștinaru, Oliviu
author_sort Babotă, Mihai
collection PubMed
description Thymus comosus Heuff ex. Griseb. (Lamiaceae) is a wild thyme species endemic for Romanian Carpathian areas, frequently collected as substitute for collective herbal product Serpylli herba, cited as antibacterial and diuretic remedy in traditional medicine. The present study aimed to evaluate the in vivo diuretic effect and in vitro antimicrobial properties of three herbal preparations (infusion—TCI, tincture—TCT and an hydroethanolic extract prepared through an optimized ultrasound-assisted method—OpTC) obtained from the aerial parts of T. comosus Heuff ex. Griseb, also evaluating their comprehensive phenolic profile. In vivo diuretic effect was tested using Wistar rats treated orally with each herbal preparation (125 and 250 mg/kg dispersed in 25 ml/kg isotonic saline solution) and quantified based on cumulative urine output (ml), diuretic action and diuretic activity. Additionally, sodium and potassium excretion were monitored using a potentiometric method with selective electrodes. In vitro antibacterial and antifungal activities were assessed using p-iodonitrotetrazolium chloride assay against six bacterial strains and six fungal strains by monitoring minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentrations (MFCs). Finally, phenolic profile of the aforementioned herbal extracts was evaluated using an ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) method to check the impact of the different preparations on the most abundant and significant compounds. All the extracts exerted a mild diuretic action, TCT and OpTC inducing the most intense diuretic effect. Both herbal preparations produced a statistically significant, dose-dependent and gradual increase of the urine output, the effect being more intense at 24 h (6.63–7.13 ml/24 h). Potentiometric evaluation of urine samples collected from treated rats revealed a clear and mild natriuretic and kaliuretic effect after the administration. In terms of antimicrobial activity, E. coli (MIC—0.38 mg/ml), B. cereus (MIC—0.75 mg/ml)), Penicillium funiculosum and P. verrucosum var. cyclopium (MIC—0.19 mg/ml) showed the greater sensitivity to the tested extracts, respectively. UHPLC-HRMS screening showed that the bioactive potential of T. comosus herbal preparations was likely related to the higher amounts of phenolic acids (including rosmarinic acid), flavonoids (mainly flavones and derivatives) and other phenolics (such as different isomers of salvianolic acids) in their composition. The obtained results support the ethnopharmacological evidence regarding the mild diuretic and antibacterial potentials of the endemic wild thyme T. comosus, this study being the first one that assessed the aforementioned bioactivities for this species.
format Online
Article
Text
id pubmed-9981668
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-99816682023-03-04 Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania Babotă, Mihai Frumuzachi, Oleg Nicolescu, Alexandru Stojković, Dejan Soković, Marina Rocchetti, Gabriele Zhang, Leilei Lucini, Luigi Crișan, Gianina Mocan, Andrei Voștinaru, Oliviu Front Pharmacol Pharmacology Thymus comosus Heuff ex. Griseb. (Lamiaceae) is a wild thyme species endemic for Romanian Carpathian areas, frequently collected as substitute for collective herbal product Serpylli herba, cited as antibacterial and diuretic remedy in traditional medicine. The present study aimed to evaluate the in vivo diuretic effect and in vitro antimicrobial properties of three herbal preparations (infusion—TCI, tincture—TCT and an hydroethanolic extract prepared through an optimized ultrasound-assisted method—OpTC) obtained from the aerial parts of T. comosus Heuff ex. Griseb, also evaluating their comprehensive phenolic profile. In vivo diuretic effect was tested using Wistar rats treated orally with each herbal preparation (125 and 250 mg/kg dispersed in 25 ml/kg isotonic saline solution) and quantified based on cumulative urine output (ml), diuretic action and diuretic activity. Additionally, sodium and potassium excretion were monitored using a potentiometric method with selective electrodes. In vitro antibacterial and antifungal activities were assessed using p-iodonitrotetrazolium chloride assay against six bacterial strains and six fungal strains by monitoring minimum inhibitory concentration (MICs), minimum bactericidal concentrations (MBCs) and minimum fungicidal concentrations (MFCs). Finally, phenolic profile of the aforementioned herbal extracts was evaluated using an ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) method to check the impact of the different preparations on the most abundant and significant compounds. All the extracts exerted a mild diuretic action, TCT and OpTC inducing the most intense diuretic effect. Both herbal preparations produced a statistically significant, dose-dependent and gradual increase of the urine output, the effect being more intense at 24 h (6.63–7.13 ml/24 h). Potentiometric evaluation of urine samples collected from treated rats revealed a clear and mild natriuretic and kaliuretic effect after the administration. In terms of antimicrobial activity, E. coli (MIC—0.38 mg/ml), B. cereus (MIC—0.75 mg/ml)), Penicillium funiculosum and P. verrucosum var. cyclopium (MIC—0.19 mg/ml) showed the greater sensitivity to the tested extracts, respectively. UHPLC-HRMS screening showed that the bioactive potential of T. comosus herbal preparations was likely related to the higher amounts of phenolic acids (including rosmarinic acid), flavonoids (mainly flavones and derivatives) and other phenolics (such as different isomers of salvianolic acids) in their composition. The obtained results support the ethnopharmacological evidence regarding the mild diuretic and antibacterial potentials of the endemic wild thyme T. comosus, this study being the first one that assessed the aforementioned bioactivities for this species. Frontiers Media S.A. 2023-02-17 /pmc/articles/PMC9981668/ /pubmed/36874013 http://dx.doi.org/10.3389/fphar.2023.1115117 Text en Copyright © 2023 Babotă, Frumuzachi, Nicolescu, Stojković, Soković, Rocchetti, Zhang, Lucini, Crișan, Mocan and Voștinaru. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Babotă, Mihai
Frumuzachi, Oleg
Nicolescu, Alexandru
Stojković, Dejan
Soković, Marina
Rocchetti, Gabriele
Zhang, Leilei
Lucini, Luigi
Crișan, Gianina
Mocan, Andrei
Voștinaru, Oliviu
Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania
title Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania
title_full Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania
title_fullStr Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania
title_full_unstemmed Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania
title_short Phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme Thymus comosus Heuff ex. Griseb. (Lamiaceae) from Romania
title_sort phenolic profile, in vitro antimicrobial and in vivo diuretic effects of endemic wild thyme thymus comosus heuff ex. griseb. (lamiaceae) from romania
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981668/
https://www.ncbi.nlm.nih.gov/pubmed/36874013
http://dx.doi.org/10.3389/fphar.2023.1115117
work_keys_str_mv AT babotamihai phenolicprofileinvitroantimicrobialandinvivodiureticeffectsofendemicwildthymethymuscomosusheuffexgriseblamiaceaefromromania
AT frumuzachioleg phenolicprofileinvitroantimicrobialandinvivodiureticeffectsofendemicwildthymethymuscomosusheuffexgriseblamiaceaefromromania
AT nicolescualexandru phenolicprofileinvitroantimicrobialandinvivodiureticeffectsofendemicwildthymethymuscomosusheuffexgriseblamiaceaefromromania
AT stojkovicdejan phenolicprofileinvitroantimicrobialandinvivodiureticeffectsofendemicwildthymethymuscomosusheuffexgriseblamiaceaefromromania
AT sokovicmarina phenolicprofileinvitroantimicrobialandinvivodiureticeffectsofendemicwildthymethymuscomosusheuffexgriseblamiaceaefromromania
AT rocchettigabriele phenolicprofileinvitroantimicrobialandinvivodiureticeffectsofendemicwildthymethymuscomosusheuffexgriseblamiaceaefromromania
AT zhangleilei phenolicprofileinvitroantimicrobialandinvivodiureticeffectsofendemicwildthymethymuscomosusheuffexgriseblamiaceaefromromania
AT luciniluigi phenolicprofileinvitroantimicrobialandinvivodiureticeffectsofendemicwildthymethymuscomosusheuffexgriseblamiaceaefromromania
AT crisangianina phenolicprofileinvitroantimicrobialandinvivodiureticeffectsofendemicwildthymethymuscomosusheuffexgriseblamiaceaefromromania
AT mocanandrei phenolicprofileinvitroantimicrobialandinvivodiureticeffectsofendemicwildthymethymuscomosusheuffexgriseblamiaceaefromromania
AT vostinaruoliviu phenolicprofileinvitroantimicrobialandinvivodiureticeffectsofendemicwildthymethymuscomosusheuffexgriseblamiaceaefromromania