Transfection of clMagR/clCry4 imparts MR-T(2) imaging contrast properties to living organisms (E. coli) in the presence of Fe(3+) by endogenous formation of iron oxide nanoparticles

Rapid development of medical imaging, such as cellular tracking, has increased the demand for “live” contrast agents. This study provides the first experimental evidence demonstrating that transfection of the clMagR/clCry4 gene can impart magnetic resonance imaging (MRI) T(2)-contrast properties to...

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Autores principales: Li, Nuan, Xue, Le, Mai, Xiaoli, Wang, Peng, Zhu, Chenzhuo, Han, Xiaofeng, Xie, Yuanyuan, Wang, Bin, Ge, Yuqing, Zhang, Yewei, Sun, Jianfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981785/
https://www.ncbi.nlm.nih.gov/pubmed/36876047
http://dx.doi.org/10.3389/fmolb.2023.1119356
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author Li, Nuan
Xue, Le
Mai, Xiaoli
Wang, Peng
Zhu, Chenzhuo
Han, Xiaofeng
Xie, Yuanyuan
Wang, Bin
Ge, Yuqing
Zhang, Yewei
Sun, Jianfei
author_facet Li, Nuan
Xue, Le
Mai, Xiaoli
Wang, Peng
Zhu, Chenzhuo
Han, Xiaofeng
Xie, Yuanyuan
Wang, Bin
Ge, Yuqing
Zhang, Yewei
Sun, Jianfei
author_sort Li, Nuan
collection PubMed
description Rapid development of medical imaging, such as cellular tracking, has increased the demand for “live” contrast agents. This study provides the first experimental evidence demonstrating that transfection of the clMagR/clCry4 gene can impart magnetic resonance imaging (MRI) T(2)-contrast properties to living prokaryotic Escherichia coli (E. coli) in the presence of Fe(3+) through the endogenous formation of iron oxide nanoparticles. The transfected clMagR/clCry4 gene markedly promoted uptake of exogenous iron by E. coli, achieving an intracellular co-precipitation condition and formation of iron oxide nanoparticles. This study will stimulate further exploration of the biological applications of clMagR/clCry4 in imaging studies.
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spelling pubmed-99817852023-03-04 Transfection of clMagR/clCry4 imparts MR-T(2) imaging contrast properties to living organisms (E. coli) in the presence of Fe(3+) by endogenous formation of iron oxide nanoparticles Li, Nuan Xue, Le Mai, Xiaoli Wang, Peng Zhu, Chenzhuo Han, Xiaofeng Xie, Yuanyuan Wang, Bin Ge, Yuqing Zhang, Yewei Sun, Jianfei Front Mol Biosci Molecular Biosciences Rapid development of medical imaging, such as cellular tracking, has increased the demand for “live” contrast agents. This study provides the first experimental evidence demonstrating that transfection of the clMagR/clCry4 gene can impart magnetic resonance imaging (MRI) T(2)-contrast properties to living prokaryotic Escherichia coli (E. coli) in the presence of Fe(3+) through the endogenous formation of iron oxide nanoparticles. The transfected clMagR/clCry4 gene markedly promoted uptake of exogenous iron by E. coli, achieving an intracellular co-precipitation condition and formation of iron oxide nanoparticles. This study will stimulate further exploration of the biological applications of clMagR/clCry4 in imaging studies. Frontiers Media S.A. 2023-02-17 /pmc/articles/PMC9981785/ /pubmed/36876047 http://dx.doi.org/10.3389/fmolb.2023.1119356 Text en Copyright © 2023 Li, Xue, Mai, Wang, Zhu, Han, Xie, Wang, Ge, Zhang and Sun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Li, Nuan
Xue, Le
Mai, Xiaoli
Wang, Peng
Zhu, Chenzhuo
Han, Xiaofeng
Xie, Yuanyuan
Wang, Bin
Ge, Yuqing
Zhang, Yewei
Sun, Jianfei
Transfection of clMagR/clCry4 imparts MR-T(2) imaging contrast properties to living organisms (E. coli) in the presence of Fe(3+) by endogenous formation of iron oxide nanoparticles
title Transfection of clMagR/clCry4 imparts MR-T(2) imaging contrast properties to living organisms (E. coli) in the presence of Fe(3+) by endogenous formation of iron oxide nanoparticles
title_full Transfection of clMagR/clCry4 imparts MR-T(2) imaging contrast properties to living organisms (E. coli) in the presence of Fe(3+) by endogenous formation of iron oxide nanoparticles
title_fullStr Transfection of clMagR/clCry4 imparts MR-T(2) imaging contrast properties to living organisms (E. coli) in the presence of Fe(3+) by endogenous formation of iron oxide nanoparticles
title_full_unstemmed Transfection of clMagR/clCry4 imparts MR-T(2) imaging contrast properties to living organisms (E. coli) in the presence of Fe(3+) by endogenous formation of iron oxide nanoparticles
title_short Transfection of clMagR/clCry4 imparts MR-T(2) imaging contrast properties to living organisms (E. coli) in the presence of Fe(3+) by endogenous formation of iron oxide nanoparticles
title_sort transfection of clmagr/clcry4 imparts mr-t(2) imaging contrast properties to living organisms (e. coli) in the presence of fe(3+) by endogenous formation of iron oxide nanoparticles
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981785/
https://www.ncbi.nlm.nih.gov/pubmed/36876047
http://dx.doi.org/10.3389/fmolb.2023.1119356
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