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Changes in upper airways microbiota in ventilator-associated pneumonia

BACKGROUND: The role of upper airways microbiota and its association with ventilator-associated pneumonia (VAP) development in mechanically ventilated (MV) patients is unclear. Taking advantage of data collected in a prospective study aimed to assess the composition and over-time variation of upper...

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Autores principales: Alagna, Laura, Mancabelli, Leonardo, Magni, Federico, Chatenoud, Liliane, Bassi, Gabriele, Del Bianco, Silvia, Fumagalli, Roberto, Turroni, Francesca, Mangioni, Davide, Migliorino, Guglielmo M., Milani, Christian, Muscatello, Antonio, Nattino, Giovanni, Picetti, Edoardo, Pinciroli, Riccardo, Rossi, Sandra, Tonetti, Tommaso, Vargiolu, Alessia, Bandera, Alessandra, Ventura, Marco, Citerio, Giuseppe, Gori, Andrea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981834/
https://www.ncbi.nlm.nih.gov/pubmed/36862343
http://dx.doi.org/10.1186/s40635-023-00496-5
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author Alagna, Laura
Mancabelli, Leonardo
Magni, Federico
Chatenoud, Liliane
Bassi, Gabriele
Del Bianco, Silvia
Fumagalli, Roberto
Turroni, Francesca
Mangioni, Davide
Migliorino, Guglielmo M.
Milani, Christian
Muscatello, Antonio
Nattino, Giovanni
Picetti, Edoardo
Pinciroli, Riccardo
Rossi, Sandra
Tonetti, Tommaso
Vargiolu, Alessia
Bandera, Alessandra
Ventura, Marco
Citerio, Giuseppe
Gori, Andrea
author_facet Alagna, Laura
Mancabelli, Leonardo
Magni, Federico
Chatenoud, Liliane
Bassi, Gabriele
Del Bianco, Silvia
Fumagalli, Roberto
Turroni, Francesca
Mangioni, Davide
Migliorino, Guglielmo M.
Milani, Christian
Muscatello, Antonio
Nattino, Giovanni
Picetti, Edoardo
Pinciroli, Riccardo
Rossi, Sandra
Tonetti, Tommaso
Vargiolu, Alessia
Bandera, Alessandra
Ventura, Marco
Citerio, Giuseppe
Gori, Andrea
author_sort Alagna, Laura
collection PubMed
description BACKGROUND: The role of upper airways microbiota and its association with ventilator-associated pneumonia (VAP) development in mechanically ventilated (MV) patients is unclear. Taking advantage of data collected in a prospective study aimed to assess the composition and over-time variation of upper airway microbiota in patients MV for non-pulmonary reasons, we describe upper airway microbiota characteristics among VAP and NO-VAP patients. METHODS: Exploratory analysis of data collected in a prospective observational study on patients intubated for non-pulmonary conditions. Microbiota analysis (trough 16S-rRNA gene profiling) was performed on endotracheal aspirates (at intubation, T0, and after 72 h, T3) of patients with VAP (cases cohort) and a subgroup of NO-VAP patients (control cohort, matched according to total intubation time). RESULTS: Samples from 13 VAP patients and 22 NO-VAP matched controls were analyzed. At intubation (T0), patients with VAP revealed a significantly lower microbial complexity of the microbiota of the upper airways compared to NO-VAP controls (alpha diversity index of 84 ± 37 and 160 ± 102, in VAP and NO_VAP group, respectively, p-value < 0.012). Furthermore, an overall decrease in microbial diversity was observed in both groups at T3 as compared to T0. At T3, a loss of some genera (Prevotella 7, Fusobacterium, Neisseria, Escherichia–Shigella and Haemophilus) was found in VAP patients. In contrast, eight genera belonging to the Bacteroidetes, Firmicutes and Fusobacteria phyla was predominant in this group. However, it is unclear whether VAP caused dysbiosis or dysbiosis caused VAP. CONCLUSIONS: In a small sample size of intubated patients, microbial diversity at intubation was less in patients with VAP compared to patients without VAP. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40635-023-00496-5.
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spelling pubmed-99818342023-03-04 Changes in upper airways microbiota in ventilator-associated pneumonia Alagna, Laura Mancabelli, Leonardo Magni, Federico Chatenoud, Liliane Bassi, Gabriele Del Bianco, Silvia Fumagalli, Roberto Turroni, Francesca Mangioni, Davide Migliorino, Guglielmo M. Milani, Christian Muscatello, Antonio Nattino, Giovanni Picetti, Edoardo Pinciroli, Riccardo Rossi, Sandra Tonetti, Tommaso Vargiolu, Alessia Bandera, Alessandra Ventura, Marco Citerio, Giuseppe Gori, Andrea Intensive Care Med Exp Research Articles BACKGROUND: The role of upper airways microbiota and its association with ventilator-associated pneumonia (VAP) development in mechanically ventilated (MV) patients is unclear. Taking advantage of data collected in a prospective study aimed to assess the composition and over-time variation of upper airway microbiota in patients MV for non-pulmonary reasons, we describe upper airway microbiota characteristics among VAP and NO-VAP patients. METHODS: Exploratory analysis of data collected in a prospective observational study on patients intubated for non-pulmonary conditions. Microbiota analysis (trough 16S-rRNA gene profiling) was performed on endotracheal aspirates (at intubation, T0, and after 72 h, T3) of patients with VAP (cases cohort) and a subgroup of NO-VAP patients (control cohort, matched according to total intubation time). RESULTS: Samples from 13 VAP patients and 22 NO-VAP matched controls were analyzed. At intubation (T0), patients with VAP revealed a significantly lower microbial complexity of the microbiota of the upper airways compared to NO-VAP controls (alpha diversity index of 84 ± 37 and 160 ± 102, in VAP and NO_VAP group, respectively, p-value < 0.012). Furthermore, an overall decrease in microbial diversity was observed in both groups at T3 as compared to T0. At T3, a loss of some genera (Prevotella 7, Fusobacterium, Neisseria, Escherichia–Shigella and Haemophilus) was found in VAP patients. In contrast, eight genera belonging to the Bacteroidetes, Firmicutes and Fusobacteria phyla was predominant in this group. However, it is unclear whether VAP caused dysbiosis or dysbiosis caused VAP. CONCLUSIONS: In a small sample size of intubated patients, microbial diversity at intubation was less in patients with VAP compared to patients without VAP. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40635-023-00496-5. Springer International Publishing 2023-03-02 /pmc/articles/PMC9981834/ /pubmed/36862343 http://dx.doi.org/10.1186/s40635-023-00496-5 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Articles
Alagna, Laura
Mancabelli, Leonardo
Magni, Federico
Chatenoud, Liliane
Bassi, Gabriele
Del Bianco, Silvia
Fumagalli, Roberto
Turroni, Francesca
Mangioni, Davide
Migliorino, Guglielmo M.
Milani, Christian
Muscatello, Antonio
Nattino, Giovanni
Picetti, Edoardo
Pinciroli, Riccardo
Rossi, Sandra
Tonetti, Tommaso
Vargiolu, Alessia
Bandera, Alessandra
Ventura, Marco
Citerio, Giuseppe
Gori, Andrea
Changes in upper airways microbiota in ventilator-associated pneumonia
title Changes in upper airways microbiota in ventilator-associated pneumonia
title_full Changes in upper airways microbiota in ventilator-associated pneumonia
title_fullStr Changes in upper airways microbiota in ventilator-associated pneumonia
title_full_unstemmed Changes in upper airways microbiota in ventilator-associated pneumonia
title_short Changes in upper airways microbiota in ventilator-associated pneumonia
title_sort changes in upper airways microbiota in ventilator-associated pneumonia
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981834/
https://www.ncbi.nlm.nih.gov/pubmed/36862343
http://dx.doi.org/10.1186/s40635-023-00496-5
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