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Polysaccharide and ethanol extracts of Anoectochilus formosanus Hayata: Antioxidant, wound-healing, antibacterial, and cytotoxic activities

INTRODUCTION: Polysaccharide and alcohol extracts of Anoectochilus formosanus Hayata have attracted great attention as they exhibit noteworthy properties such as prebiotic and anti-hyperglycemic effects. However, the antioxidant and wound-healing activities of the polysaccharide extract as well as t...

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Autores principales: Nguyen, Thi-Phuong, Phan, Han N., Do, Thang Duc, Do, Giap Dang, Ngo, Long Hoang, Do, Hoang Dang Khoa, Nguyen, Khoa Thi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981919/
https://www.ncbi.nlm.nih.gov/pubmed/36873493
http://dx.doi.org/10.1016/j.heliyon.2023.e13559
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author Nguyen, Thi-Phuong
Phan, Han N.
Do, Thang Duc
Do, Giap Dang
Ngo, Long Hoang
Do, Hoang Dang Khoa
Nguyen, Khoa Thi
author_facet Nguyen, Thi-Phuong
Phan, Han N.
Do, Thang Duc
Do, Giap Dang
Ngo, Long Hoang
Do, Hoang Dang Khoa
Nguyen, Khoa Thi
author_sort Nguyen, Thi-Phuong
collection PubMed
description INTRODUCTION: Polysaccharide and alcohol extracts of Anoectochilus formosanus Hayata have attracted great attention as they exhibit noteworthy properties such as prebiotic and anti-hyperglycemic effects. However, the antioxidant and wound-healing activities of the polysaccharide extract as well as the antibacterial and cytotoxic effects of the ethanol extracts have not been thoroughly uncovered. Therefore, our study investigated these bioactivities of the two extracts prepared from Anoectochilus formosanus to broaden understandings of medical benefits of the plant. METHODS: The monosaccharide composition was analyzed by HPAEC-PAD. The antioxidant and wound-healing activities of the polysaccharide extract were evaluated by ABTS and scratch assays, respectively. The broth dilution method was used to determine the antibacterial ability of the ethanol extract. Additionally, the cytotoxic and mechanistic effects of this extract against hepatocellular carcinoma HUH-7 cells was assessed by MTT assay, qRT-PCR and Western blotting methods. RESULTS: The polysaccharide extract possessed an effective free radical scavenging ability in an ABTS assay (IC(50) = 44.92 μg/ml). The extract also ameliorated wound recovery in a fibroblast scratch assay. Meanwhile, the ethanol extract was able to inhibit the growth of Staphylococcus aureus (MIC = 2500 μg/ml), Bacillus cereus (MIC = 2500 μg/ml), Escherichia coli (MIC = 2500 μg/ml), and Pseudomonas aeruginosa (MIC = 1250 μg/ml). Additionally, it repressed the viability of HUH-7 cells (IC(50) = 53.44 μg/ml), possibly through upregulating the expression of caspase 3 (CASP3), CASP8, and CASP9 at both mRNA and protein levels. CONCLUSION: The polysaccharide extract of A. formosanus exhibited the antioxidant and wound-healing properties whereas the ethanol extract showed the antibacterial activity and cytotoxicity against HUH-7 cells. These findings specify notable biological effects of the two extracts which could be of potential use in human healthcare.
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spelling pubmed-99819192023-03-04 Polysaccharide and ethanol extracts of Anoectochilus formosanus Hayata: Antioxidant, wound-healing, antibacterial, and cytotoxic activities Nguyen, Thi-Phuong Phan, Han N. Do, Thang Duc Do, Giap Dang Ngo, Long Hoang Do, Hoang Dang Khoa Nguyen, Khoa Thi Heliyon Research Article INTRODUCTION: Polysaccharide and alcohol extracts of Anoectochilus formosanus Hayata have attracted great attention as they exhibit noteworthy properties such as prebiotic and anti-hyperglycemic effects. However, the antioxidant and wound-healing activities of the polysaccharide extract as well as the antibacterial and cytotoxic effects of the ethanol extracts have not been thoroughly uncovered. Therefore, our study investigated these bioactivities of the two extracts prepared from Anoectochilus formosanus to broaden understandings of medical benefits of the plant. METHODS: The monosaccharide composition was analyzed by HPAEC-PAD. The antioxidant and wound-healing activities of the polysaccharide extract were evaluated by ABTS and scratch assays, respectively. The broth dilution method was used to determine the antibacterial ability of the ethanol extract. Additionally, the cytotoxic and mechanistic effects of this extract against hepatocellular carcinoma HUH-7 cells was assessed by MTT assay, qRT-PCR and Western blotting methods. RESULTS: The polysaccharide extract possessed an effective free radical scavenging ability in an ABTS assay (IC(50) = 44.92 μg/ml). The extract also ameliorated wound recovery in a fibroblast scratch assay. Meanwhile, the ethanol extract was able to inhibit the growth of Staphylococcus aureus (MIC = 2500 μg/ml), Bacillus cereus (MIC = 2500 μg/ml), Escherichia coli (MIC = 2500 μg/ml), and Pseudomonas aeruginosa (MIC = 1250 μg/ml). Additionally, it repressed the viability of HUH-7 cells (IC(50) = 53.44 μg/ml), possibly through upregulating the expression of caspase 3 (CASP3), CASP8, and CASP9 at both mRNA and protein levels. CONCLUSION: The polysaccharide extract of A. formosanus exhibited the antioxidant and wound-healing properties whereas the ethanol extract showed the antibacterial activity and cytotoxicity against HUH-7 cells. These findings specify notable biological effects of the two extracts which could be of potential use in human healthcare. Elsevier 2023-02-14 /pmc/articles/PMC9981919/ /pubmed/36873493 http://dx.doi.org/10.1016/j.heliyon.2023.e13559 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Nguyen, Thi-Phuong
Phan, Han N.
Do, Thang Duc
Do, Giap Dang
Ngo, Long Hoang
Do, Hoang Dang Khoa
Nguyen, Khoa Thi
Polysaccharide and ethanol extracts of Anoectochilus formosanus Hayata: Antioxidant, wound-healing, antibacterial, and cytotoxic activities
title Polysaccharide and ethanol extracts of Anoectochilus formosanus Hayata: Antioxidant, wound-healing, antibacterial, and cytotoxic activities
title_full Polysaccharide and ethanol extracts of Anoectochilus formosanus Hayata: Antioxidant, wound-healing, antibacterial, and cytotoxic activities
title_fullStr Polysaccharide and ethanol extracts of Anoectochilus formosanus Hayata: Antioxidant, wound-healing, antibacterial, and cytotoxic activities
title_full_unstemmed Polysaccharide and ethanol extracts of Anoectochilus formosanus Hayata: Antioxidant, wound-healing, antibacterial, and cytotoxic activities
title_short Polysaccharide and ethanol extracts of Anoectochilus formosanus Hayata: Antioxidant, wound-healing, antibacterial, and cytotoxic activities
title_sort polysaccharide and ethanol extracts of anoectochilus formosanus hayata: antioxidant, wound-healing, antibacterial, and cytotoxic activities
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9981919/
https://www.ncbi.nlm.nih.gov/pubmed/36873493
http://dx.doi.org/10.1016/j.heliyon.2023.e13559
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