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Terahertz spectra of proteinuria and non-proteinuria

In clinical practice, proteinuria detection is of great significance in the diagnosis of kidney diseases. Dipstick analysis is used in most outpatient settings to semi-quantitatively measure the urine protein concentration. However, this method has limitations for protein detection, and alkaline uri...

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Autores principales: Xue, Zhenrui, Mao, Ping, Peng, Ping, Yan, Shihan, Zang, Ziyi, Yao, Chunyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9982117/
https://www.ncbi.nlm.nih.gov/pubmed/36873349
http://dx.doi.org/10.3389/fbioe.2023.1119694
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author Xue, Zhenrui
Mao, Ping
Peng, Ping
Yan, Shihan
Zang, Ziyi
Yao, Chunyan
author_facet Xue, Zhenrui
Mao, Ping
Peng, Ping
Yan, Shihan
Zang, Ziyi
Yao, Chunyan
author_sort Xue, Zhenrui
collection PubMed
description In clinical practice, proteinuria detection is of great significance in the diagnosis of kidney diseases. Dipstick analysis is used in most outpatient settings to semi-quantitatively measure the urine protein concentration. However, this method has limitations for protein detection, and alkaline urine or hematuria will cause false positive results. Recently, terahertz time-domain spectroscopy (THz-TDS) with strong hydrogen bonding sensitivity has been proven to be able to distinguish different types of biological solutions, which means that protein molecules in urine may have different THz spectral characteristics. In this study, we performed a preliminary clinical study investigating the terahertz spectra of 20 fresh urine samples (non-proteinuria and proteinuria). The results showed that the concentration of urine protein was positively correlated with the absorption of THz spectra at 0.5–1.2 THz. At 1.0 THz, the pH values (6, 7, 8, and 9) had no significant effect on the THz absorption spectra of urine proteins. The terahertz absorption of proteins with a high molecular weight (albumin) was greater than that of proteins with a low molecular weight (β2-microglobulin) at the same concentration. Overall, THz-TDS spectroscopy for the qualitative detection of proteinuria is not affected by pH and has the potential to discriminate between albumin and β2-microglobulin in urine.
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spelling pubmed-99821172023-03-04 Terahertz spectra of proteinuria and non-proteinuria Xue, Zhenrui Mao, Ping Peng, Ping Yan, Shihan Zang, Ziyi Yao, Chunyan Front Bioeng Biotechnol Bioengineering and Biotechnology In clinical practice, proteinuria detection is of great significance in the diagnosis of kidney diseases. Dipstick analysis is used in most outpatient settings to semi-quantitatively measure the urine protein concentration. However, this method has limitations for protein detection, and alkaline urine or hematuria will cause false positive results. Recently, terahertz time-domain spectroscopy (THz-TDS) with strong hydrogen bonding sensitivity has been proven to be able to distinguish different types of biological solutions, which means that protein molecules in urine may have different THz spectral characteristics. In this study, we performed a preliminary clinical study investigating the terahertz spectra of 20 fresh urine samples (non-proteinuria and proteinuria). The results showed that the concentration of urine protein was positively correlated with the absorption of THz spectra at 0.5–1.2 THz. At 1.0 THz, the pH values (6, 7, 8, and 9) had no significant effect on the THz absorption spectra of urine proteins. The terahertz absorption of proteins with a high molecular weight (albumin) was greater than that of proteins with a low molecular weight (β2-microglobulin) at the same concentration. Overall, THz-TDS spectroscopy for the qualitative detection of proteinuria is not affected by pH and has the potential to discriminate between albumin and β2-microglobulin in urine. Frontiers Media S.A. 2023-02-17 /pmc/articles/PMC9982117/ /pubmed/36873349 http://dx.doi.org/10.3389/fbioe.2023.1119694 Text en Copyright © 2023 Xue, Mao, Peng, Yan, Zang and Yao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Xue, Zhenrui
Mao, Ping
Peng, Ping
Yan, Shihan
Zang, Ziyi
Yao, Chunyan
Terahertz spectra of proteinuria and non-proteinuria
title Terahertz spectra of proteinuria and non-proteinuria
title_full Terahertz spectra of proteinuria and non-proteinuria
title_fullStr Terahertz spectra of proteinuria and non-proteinuria
title_full_unstemmed Terahertz spectra of proteinuria and non-proteinuria
title_short Terahertz spectra of proteinuria and non-proteinuria
title_sort terahertz spectra of proteinuria and non-proteinuria
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9982117/
https://www.ncbi.nlm.nih.gov/pubmed/36873349
http://dx.doi.org/10.3389/fbioe.2023.1119694
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