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M13 phage: a versatile building block for a highly specific analysis platform
Viruses are changing the biosensing and biomedicine landscape due to their multivalency, orthogonal reactivities, and responsiveness to genetic modifications. As the most extensively studied phage model for constructing a phage display library, M13 phage has received much research attention as build...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9982796/ https://www.ncbi.nlm.nih.gov/pubmed/36867197 http://dx.doi.org/10.1007/s00216-023-04606-w |
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author | Wang, Rui Li, Hui-Da Cao, Ying Wang, Zi-Yi Yang, Ting Wang, Jian-Hua |
author_facet | Wang, Rui Li, Hui-Da Cao, Ying Wang, Zi-Yi Yang, Ting Wang, Jian-Hua |
author_sort | Wang, Rui |
collection | PubMed |
description | Viruses are changing the biosensing and biomedicine landscape due to their multivalency, orthogonal reactivities, and responsiveness to genetic modifications. As the most extensively studied phage model for constructing a phage display library, M13 phage has received much research attention as building blocks or viral scaffolds for various applications including isolation/separation, sensing/probing, and in vivo imaging. Through genetic engineering and chemical modification, M13 phages can be functionalized into a multifunctional analysis platform with various functional regions conducting their functionality without mutual disturbance. Its unique filamentous morphology and flexibility also promoted the analytical performance in terms of target affinity and signal amplification. In this review, we mainly focused on the application of M13 phage in the analytical field and the benefit it brings. We also introduced several genetic engineering and chemical modification approaches for endowing M13 with various functionalities, and summarized some representative applications using M13 phages to construct isolation sorbents, biosensors, cell imaging probes, and immunoassays. Finally, current issues and challenges remaining in this field were discussed and future perspectives were also proposed. GRAPHICAL ABSTRACT: [Image: see text] |
format | Online Article Text |
id | pubmed-9982796 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-99827962023-03-03 M13 phage: a versatile building block for a highly specific analysis platform Wang, Rui Li, Hui-Da Cao, Ying Wang, Zi-Yi Yang, Ting Wang, Jian-Hua Anal Bioanal Chem Critical Review Viruses are changing the biosensing and biomedicine landscape due to their multivalency, orthogonal reactivities, and responsiveness to genetic modifications. As the most extensively studied phage model for constructing a phage display library, M13 phage has received much research attention as building blocks or viral scaffolds for various applications including isolation/separation, sensing/probing, and in vivo imaging. Through genetic engineering and chemical modification, M13 phages can be functionalized into a multifunctional analysis platform with various functional regions conducting their functionality without mutual disturbance. Its unique filamentous morphology and flexibility also promoted the analytical performance in terms of target affinity and signal amplification. In this review, we mainly focused on the application of M13 phage in the analytical field and the benefit it brings. We also introduced several genetic engineering and chemical modification approaches for endowing M13 with various functionalities, and summarized some representative applications using M13 phages to construct isolation sorbents, biosensors, cell imaging probes, and immunoassays. Finally, current issues and challenges remaining in this field were discussed and future perspectives were also proposed. GRAPHICAL ABSTRACT: [Image: see text] Springer Berlin Heidelberg 2023-03-03 /pmc/articles/PMC9982796/ /pubmed/36867197 http://dx.doi.org/10.1007/s00216-023-04606-w Text en © Springer-Verlag GmbH Germany, part of Springer Nature 2023, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Critical Review Wang, Rui Li, Hui-Da Cao, Ying Wang, Zi-Yi Yang, Ting Wang, Jian-Hua M13 phage: a versatile building block for a highly specific analysis platform |
title | M13 phage: a versatile building block for a highly specific analysis platform |
title_full | M13 phage: a versatile building block for a highly specific analysis platform |
title_fullStr | M13 phage: a versatile building block for a highly specific analysis platform |
title_full_unstemmed | M13 phage: a versatile building block for a highly specific analysis platform |
title_short | M13 phage: a versatile building block for a highly specific analysis platform |
title_sort | m13 phage: a versatile building block for a highly specific analysis platform |
topic | Critical Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9982796/ https://www.ncbi.nlm.nih.gov/pubmed/36867197 http://dx.doi.org/10.1007/s00216-023-04606-w |
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