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ASPP2 suppresses tumour growth and stemness characteristics in HCC by inhibiting Warburg effect via WNT/β‐catenin/HK2 axis

Abnormal energy metabolism is one of the characteristics of tumours. In the last few years, more and more attention is being paid to the role and regulation of tumour aerobic glycolysis. Cancer cells display enhanced aerobic glycolysis, also known as the Warburg effect, whereby tumour cells absorb g...

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Autores principales: Liang, Beibei, Jiang, Yuan, Song, Shaohua, Jing, Wei, Yang, Hao, Zhao, Li, Chen, Ya, Tang, Qiqi, Li, Xuhui, Zhang, Lisha, Bao, Haili, Huang, Gang, Zhao, Jian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9983321/
https://www.ncbi.nlm.nih.gov/pubmed/36752127
http://dx.doi.org/10.1111/jcmm.17687
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author Liang, Beibei
Jiang, Yuan
Song, Shaohua
Jing, Wei
Yang, Hao
Zhao, Li
Chen, Ya
Tang, Qiqi
Li, Xuhui
Zhang, Lisha
Bao, Haili
Huang, Gang
Zhao, Jian
author_facet Liang, Beibei
Jiang, Yuan
Song, Shaohua
Jing, Wei
Yang, Hao
Zhao, Li
Chen, Ya
Tang, Qiqi
Li, Xuhui
Zhang, Lisha
Bao, Haili
Huang, Gang
Zhao, Jian
author_sort Liang, Beibei
collection PubMed
description Abnormal energy metabolism is one of the characteristics of tumours. In the last few years, more and more attention is being paid to the role and regulation of tumour aerobic glycolysis. Cancer cells display enhanced aerobic glycolysis, also known as the Warburg effect, whereby tumour cells absorb glucose to produce a large amount of lactic acid and energy under aerobic conditions to favour tumour proliferation and metastasis. In this study, we report that the haploinsufficient tumour suppressor ASPP2, can inhibit HCC growth and stemness characteristics by regulating the Warburg effect through the WNT/β‐catenin pathway. we performed glucose uptake, lactate production, pyruvate production, ECAR and OCR assays to verify ASPP2 can inhibit glycolysis in HCC cells. The expression of ASPP2 and HK2 was significantly inversely correlated in 80 HCC tissues. Our study reveals downregulation of ASPP2 can promote the aerobic glycolysis metabolism pathway, increasing HCC proliferation, glycolysis metabolism, stemness and drug resistance. This ASPP2‐induced inhibition of glycolysis metabolism depends on the WNT/β‐catenin pathway. ASPP2‐regulated Warburg effect is associated with tumour progression and provides prognostic value. and suggest that may be promising as a new therapeutic strategy in HCC.
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spelling pubmed-99833212023-03-04 ASPP2 suppresses tumour growth and stemness characteristics in HCC by inhibiting Warburg effect via WNT/β‐catenin/HK2 axis Liang, Beibei Jiang, Yuan Song, Shaohua Jing, Wei Yang, Hao Zhao, Li Chen, Ya Tang, Qiqi Li, Xuhui Zhang, Lisha Bao, Haili Huang, Gang Zhao, Jian J Cell Mol Med Original Articles Abnormal energy metabolism is one of the characteristics of tumours. In the last few years, more and more attention is being paid to the role and regulation of tumour aerobic glycolysis. Cancer cells display enhanced aerobic glycolysis, also known as the Warburg effect, whereby tumour cells absorb glucose to produce a large amount of lactic acid and energy under aerobic conditions to favour tumour proliferation and metastasis. In this study, we report that the haploinsufficient tumour suppressor ASPP2, can inhibit HCC growth and stemness characteristics by regulating the Warburg effect through the WNT/β‐catenin pathway. we performed glucose uptake, lactate production, pyruvate production, ECAR and OCR assays to verify ASPP2 can inhibit glycolysis in HCC cells. The expression of ASPP2 and HK2 was significantly inversely correlated in 80 HCC tissues. Our study reveals downregulation of ASPP2 can promote the aerobic glycolysis metabolism pathway, increasing HCC proliferation, glycolysis metabolism, stemness and drug resistance. This ASPP2‐induced inhibition of glycolysis metabolism depends on the WNT/β‐catenin pathway. ASPP2‐regulated Warburg effect is associated with tumour progression and provides prognostic value. and suggest that may be promising as a new therapeutic strategy in HCC. John Wiley and Sons Inc. 2023-02-08 /pmc/articles/PMC9983321/ /pubmed/36752127 http://dx.doi.org/10.1111/jcmm.17687 Text en © 2023 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Liang, Beibei
Jiang, Yuan
Song, Shaohua
Jing, Wei
Yang, Hao
Zhao, Li
Chen, Ya
Tang, Qiqi
Li, Xuhui
Zhang, Lisha
Bao, Haili
Huang, Gang
Zhao, Jian
ASPP2 suppresses tumour growth and stemness characteristics in HCC by inhibiting Warburg effect via WNT/β‐catenin/HK2 axis
title ASPP2 suppresses tumour growth and stemness characteristics in HCC by inhibiting Warburg effect via WNT/β‐catenin/HK2 axis
title_full ASPP2 suppresses tumour growth and stemness characteristics in HCC by inhibiting Warburg effect via WNT/β‐catenin/HK2 axis
title_fullStr ASPP2 suppresses tumour growth and stemness characteristics in HCC by inhibiting Warburg effect via WNT/β‐catenin/HK2 axis
title_full_unstemmed ASPP2 suppresses tumour growth and stemness characteristics in HCC by inhibiting Warburg effect via WNT/β‐catenin/HK2 axis
title_short ASPP2 suppresses tumour growth and stemness characteristics in HCC by inhibiting Warburg effect via WNT/β‐catenin/HK2 axis
title_sort aspp2 suppresses tumour growth and stemness characteristics in hcc by inhibiting warburg effect via wnt/β‐catenin/hk2 axis
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9983321/
https://www.ncbi.nlm.nih.gov/pubmed/36752127
http://dx.doi.org/10.1111/jcmm.17687
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