Cargando…
Effect of dexmedetomidine on tourniquet-induced skeletal muscle injury
OBJECTIVE: The aim of this study was to investigate whether dexmedetomidine could reduce tourniquet-induced skeletal muscle injury. METHODS: C57BL6 male mice were randomly assigned to sham, ischemia/reperfusion, and dexmedetomidine groups. Mice in the ischemia/reperfusion and dexmedetomidine groups...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Médica Brasileira
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9983473/ https://www.ncbi.nlm.nih.gov/pubmed/36888762 http://dx.doi.org/10.1590/1806-9282.20220865 |
_version_ | 1784900555021221888 |
---|---|
author | Cheng, Wenjie Wu, Zhe Zhang, Jizheng Ren, Wanlu |
author_facet | Cheng, Wenjie Wu, Zhe Zhang, Jizheng Ren, Wanlu |
author_sort | Cheng, Wenjie |
collection | PubMed |
description | OBJECTIVE: The aim of this study was to investigate whether dexmedetomidine could reduce tourniquet-induced skeletal muscle injury. METHODS: C57BL6 male mice were randomly assigned to sham, ischemia/reperfusion, and dexmedetomidine groups. Mice in the ischemia/reperfusion and dexmedetomidine groups received normal saline solution and dexmedetomidine intraperitoneally, respectively. The sham group underwent the same procedure as the ischemia/reperfusion group, with the exception of tourniquet application. Subsequently, the ultrastructure of the gastrocnemius muscle was observed, and its contractile force was examined. In addition, Toll-like receptor 4 and nuclear factor-κB expression within muscles was detected by Western blot. RESULTS: Dexmedetomidine alleviated myocyte damage and increased the contractility of skeletal muscles. Moreover, dexmedetomidine significantly inhibited the expression of Toll-like receptor 4/nuclear factor-κB in the gastrocnemius muscle. CONCLUSION: Taken together, these results demonstrate that dexmedetomidine administration attenuated tourniquet-induced structural and functional impairment of the skeletal muscle, partly through inactivation of the Toll-like receptor 4/nuclear factor-κB pathway. |
format | Online Article Text |
id | pubmed-9983473 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Associação Médica Brasileira |
record_format | MEDLINE/PubMed |
spelling | pubmed-99834732023-03-04 Effect of dexmedetomidine on tourniquet-induced skeletal muscle injury Cheng, Wenjie Wu, Zhe Zhang, Jizheng Ren, Wanlu Rev Assoc Med Bras (1992) Original Article OBJECTIVE: The aim of this study was to investigate whether dexmedetomidine could reduce tourniquet-induced skeletal muscle injury. METHODS: C57BL6 male mice were randomly assigned to sham, ischemia/reperfusion, and dexmedetomidine groups. Mice in the ischemia/reperfusion and dexmedetomidine groups received normal saline solution and dexmedetomidine intraperitoneally, respectively. The sham group underwent the same procedure as the ischemia/reperfusion group, with the exception of tourniquet application. Subsequently, the ultrastructure of the gastrocnemius muscle was observed, and its contractile force was examined. In addition, Toll-like receptor 4 and nuclear factor-κB expression within muscles was detected by Western blot. RESULTS: Dexmedetomidine alleviated myocyte damage and increased the contractility of skeletal muscles. Moreover, dexmedetomidine significantly inhibited the expression of Toll-like receptor 4/nuclear factor-κB in the gastrocnemius muscle. CONCLUSION: Taken together, these results demonstrate that dexmedetomidine administration attenuated tourniquet-induced structural and functional impairment of the skeletal muscle, partly through inactivation of the Toll-like receptor 4/nuclear factor-κB pathway. Associação Médica Brasileira 2023-03-03 /pmc/articles/PMC9983473/ /pubmed/36888762 http://dx.doi.org/10.1590/1806-9282.20220865 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Cheng, Wenjie Wu, Zhe Zhang, Jizheng Ren, Wanlu Effect of dexmedetomidine on tourniquet-induced skeletal muscle injury |
title | Effect of dexmedetomidine on tourniquet-induced skeletal muscle injury |
title_full | Effect of dexmedetomidine on tourniquet-induced skeletal muscle injury |
title_fullStr | Effect of dexmedetomidine on tourniquet-induced skeletal muscle injury |
title_full_unstemmed | Effect of dexmedetomidine on tourniquet-induced skeletal muscle injury |
title_short | Effect of dexmedetomidine on tourniquet-induced skeletal muscle injury |
title_sort | effect of dexmedetomidine on tourniquet-induced skeletal muscle injury |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9983473/ https://www.ncbi.nlm.nih.gov/pubmed/36888762 http://dx.doi.org/10.1590/1806-9282.20220865 |
work_keys_str_mv | AT chengwenjie effectofdexmedetomidineontourniquetinducedskeletalmuscleinjury AT wuzhe effectofdexmedetomidineontourniquetinducedskeletalmuscleinjury AT zhangjizheng effectofdexmedetomidineontourniquetinducedskeletalmuscleinjury AT renwanlu effectofdexmedetomidineontourniquetinducedskeletalmuscleinjury |