Cargando…
Quantitative analysis of C. elegans transcripts by Nanopore direct-cDNA sequencing reveals terminal hairpins in non trans-spliced mRNAs
In nematodes and kinetoplastids, mRNA processing involves a trans-splicing step through which a short sequence from a snRNP replaces the original 5’ end of the primary transcript. It has long been held that 70% of C. elegans mRNAs are submitted to trans-splicing. Our recent work suggested that the m...
Autores principales: | Bernard, Florian, Dargère, Delphine, Rechavi, Oded, Dupuy, Denis |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9984361/ https://www.ncbi.nlm.nih.gov/pubmed/36869073 http://dx.doi.org/10.1038/s41467-023-36915-0 |
Ejemplares similares
-
Expression of a human cDNA in moss results in spliced mRNAs and fragmentary protein isoforms
por: Top, Oguz, et al.
Publicado: (2021) -
Trans-splicing enhances translational efficiency in C. elegans
por: Yang, Yu-Fei, et al.
Publicado: (2017) -
U6 snRNA m6A modification is required for accurate and efficient cis- and trans-splicing of C. elegans mRNAs
por: Shen, Aykut, et al.
Publicado: (2023) -
Nanopore sequencing of RNA and cDNA molecules in Escherichia coli
por: Grünberger, Felix, et al.
Publicado: (2022) -
Improved Nanopore full-length cDNA sequencing by PCR-suppression
por: Bayega, Anthony, et al.
Publicado: (2022)