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Inactivation of Giardia intestinalis cysts in water using a sonochemical method based on mid-high-frequency waves

Giardia intestinalis is a pollutant of food and water, resistant to conventional disinfection treatments and its elimination requires effective methods action. Herein, mid-high-frequency ultrasound (375 kHz), which produces HO• and H(2)O(2), was used as an alternative method of treatment to inactiva...

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Autores principales: Gómez-Quintero, Laura María, Múnera-Marín, Marlon Alexis, Urán-Serna, María Alejandra, Serna-Galvis, Efraím A., Galván-Diaz, Ana Luz, Torres-Palma, Ricardo A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9984674/
https://www.ncbi.nlm.nih.gov/pubmed/36879762
http://dx.doi.org/10.1016/j.mex.2023.102068
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author Gómez-Quintero, Laura María
Múnera-Marín, Marlon Alexis
Urán-Serna, María Alejandra
Serna-Galvis, Efraím A.
Galván-Diaz, Ana Luz
Torres-Palma, Ricardo A.
author_facet Gómez-Quintero, Laura María
Múnera-Marín, Marlon Alexis
Urán-Serna, María Alejandra
Serna-Galvis, Efraím A.
Galván-Diaz, Ana Luz
Torres-Palma, Ricardo A.
author_sort Gómez-Quintero, Laura María
collection PubMed
description Giardia intestinalis is a pollutant of food and water, resistant to conventional disinfection treatments and its elimination requires effective methods action. Herein, mid-high-frequency ultrasound (375 kHz), which produces HO• and H(2)O(2), was used as an alternative method of treatment to inactivate Giardia intestinalis cysts in water. The effect of ultrasound power (4.0, 11.2, 24.4 W) on the sonogeneration of radicals was tested, showing that 24.4 W was the condition most favorable to treat the parasite. The viability of the protozoan cysts was evaluated using the immunofluorescence technique and vital stains, showing this protocol was useful to quantify the parasite. The sonochemical method (at 375 kHz and 24.4 W) was applied at different treatment times (10, 20, and 40 min). A significant decrease in the protozoan concentration (reduction of 52.4% of viable cysts) was observed after 20 min of treatment. However, the extension of treatment time up to 40 min did not increase the inactivation. Disinfecting action was associated with attacks on the Giardia intestinalis cyst by sonogenerated HO• and H(2)O(2) • Mid-high-frequency ultrasound produces HO• and H(2)O(2) profitable to inactivate Giardia intestinalis. • Immunofluorescence technique and vital stains allowed us to quantify the parasite viability. • Giardia intestinalis cysts concentration decreased by 52.4% after only 20 min of sonication.
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spelling pubmed-99846742023-03-05 Inactivation of Giardia intestinalis cysts in water using a sonochemical method based on mid-high-frequency waves Gómez-Quintero, Laura María Múnera-Marín, Marlon Alexis Urán-Serna, María Alejandra Serna-Galvis, Efraím A. Galván-Diaz, Ana Luz Torres-Palma, Ricardo A. MethodsX Method Article Giardia intestinalis is a pollutant of food and water, resistant to conventional disinfection treatments and its elimination requires effective methods action. Herein, mid-high-frequency ultrasound (375 kHz), which produces HO• and H(2)O(2), was used as an alternative method of treatment to inactivate Giardia intestinalis cysts in water. The effect of ultrasound power (4.0, 11.2, 24.4 W) on the sonogeneration of radicals was tested, showing that 24.4 W was the condition most favorable to treat the parasite. The viability of the protozoan cysts was evaluated using the immunofluorescence technique and vital stains, showing this protocol was useful to quantify the parasite. The sonochemical method (at 375 kHz and 24.4 W) was applied at different treatment times (10, 20, and 40 min). A significant decrease in the protozoan concentration (reduction of 52.4% of viable cysts) was observed after 20 min of treatment. However, the extension of treatment time up to 40 min did not increase the inactivation. Disinfecting action was associated with attacks on the Giardia intestinalis cyst by sonogenerated HO• and H(2)O(2) • Mid-high-frequency ultrasound produces HO• and H(2)O(2) profitable to inactivate Giardia intestinalis. • Immunofluorescence technique and vital stains allowed us to quantify the parasite viability. • Giardia intestinalis cysts concentration decreased by 52.4% after only 20 min of sonication. Elsevier 2023-02-09 /pmc/articles/PMC9984674/ /pubmed/36879762 http://dx.doi.org/10.1016/j.mex.2023.102068 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Method Article
Gómez-Quintero, Laura María
Múnera-Marín, Marlon Alexis
Urán-Serna, María Alejandra
Serna-Galvis, Efraím A.
Galván-Diaz, Ana Luz
Torres-Palma, Ricardo A.
Inactivation of Giardia intestinalis cysts in water using a sonochemical method based on mid-high-frequency waves
title Inactivation of Giardia intestinalis cysts in water using a sonochemical method based on mid-high-frequency waves
title_full Inactivation of Giardia intestinalis cysts in water using a sonochemical method based on mid-high-frequency waves
title_fullStr Inactivation of Giardia intestinalis cysts in water using a sonochemical method based on mid-high-frequency waves
title_full_unstemmed Inactivation of Giardia intestinalis cysts in water using a sonochemical method based on mid-high-frequency waves
title_short Inactivation of Giardia intestinalis cysts in water using a sonochemical method based on mid-high-frequency waves
title_sort inactivation of giardia intestinalis cysts in water using a sonochemical method based on mid-high-frequency waves
topic Method Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9984674/
https://www.ncbi.nlm.nih.gov/pubmed/36879762
http://dx.doi.org/10.1016/j.mex.2023.102068
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