Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair process

Organs-on-chips are microfluidic devices for cell culturing to simulate tissue- or organ-level physiology, providing new solutions other than traditional animal tests. Here, we describe a microfluidic platform consisting of human corneal cells and compartmentalizing channels to achieve fully integra...

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Detalles Bibliográficos
Autores principales: Yu, Zitong, Hao, Rui, Chen, Xi, Ma, Lu, Zhang, Yi, Yang, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9984679/
https://www.ncbi.nlm.nih.gov/pubmed/36861830
http://dx.doi.org/10.1016/j.xpro.2023.102122
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author Yu, Zitong
Hao, Rui
Chen, Xi
Ma, Lu
Zhang, Yi
Yang, Hui
author_facet Yu, Zitong
Hao, Rui
Chen, Xi
Ma, Lu
Zhang, Yi
Yang, Hui
author_sort Yu, Zitong
collection PubMed
description Organs-on-chips are microfluidic devices for cell culturing to simulate tissue- or organ-level physiology, providing new solutions other than traditional animal tests. Here, we describe a microfluidic platform consisting of human corneal cells and compartmentalizing channels to achieve fully integrated human cornea’s barrier effects on the chip. We detail steps to verify the barrier effects and physiological phenotypes of microengineered human cornea. Then, we use the platform to evaluate the corneal epithelial wound repair process. For complete details on the use and execution of this protocol, please refer to Yu et al. (2022).(1)
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spelling pubmed-99846792023-03-05 Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair process Yu, Zitong Hao, Rui Chen, Xi Ma, Lu Zhang, Yi Yang, Hui STAR Protoc Protocol Organs-on-chips are microfluidic devices for cell culturing to simulate tissue- or organ-level physiology, providing new solutions other than traditional animal tests. Here, we describe a microfluidic platform consisting of human corneal cells and compartmentalizing channels to achieve fully integrated human cornea’s barrier effects on the chip. We detail steps to verify the barrier effects and physiological phenotypes of microengineered human cornea. Then, we use the platform to evaluate the corneal epithelial wound repair process. For complete details on the use and execution of this protocol, please refer to Yu et al. (2022).(1) Elsevier 2023-02-15 /pmc/articles/PMC9984679/ /pubmed/36861830 http://dx.doi.org/10.1016/j.xpro.2023.102122 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Yu, Zitong
Hao, Rui
Chen, Xi
Ma, Lu
Zhang, Yi
Yang, Hui
Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair process
title Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair process
title_full Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair process
title_fullStr Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair process
title_full_unstemmed Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair process
title_short Protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair process
title_sort protocol to develop a microfluidic human corneal barrier-on-a-chip to evaluate the corneal epithelial wound repair process
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9984679/
https://www.ncbi.nlm.nih.gov/pubmed/36861830
http://dx.doi.org/10.1016/j.xpro.2023.102122
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