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Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants

Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target...

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Autores principales: Gutiérrez-Martín, Daniel, Marquès, Montse, Pons-Escoda, Albert, Vidal, Noemi, Bruna, Jordi, Restrepo-Montes, Esteban, López-Serna, Rebeca, García-Sayago, Francisco, Majos, Carles, Gago-Ferrero, Pablo, Gil-Solsona, Rubén
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9984680/
https://www.ncbi.nlm.nih.gov/pubmed/36879761
http://dx.doi.org/10.1016/j.mex.2023.102069
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author Gutiérrez-Martín, Daniel
Marquès, Montse
Pons-Escoda, Albert
Vidal, Noemi
Bruna, Jordi
Restrepo-Montes, Esteban
López-Serna, Rebeca
García-Sayago, Francisco
Majos, Carles
Gago-Ferrero, Pablo
Gil-Solsona, Rubén
author_facet Gutiérrez-Martín, Daniel
Marquès, Montse
Pons-Escoda, Albert
Vidal, Noemi
Bruna, Jordi
Restrepo-Montes, Esteban
López-Serna, Rebeca
García-Sayago, Francisco
Majos, Carles
Gago-Ferrero, Pablo
Gil-Solsona, Rubén
author_sort Gutiérrez-Martín, Daniel
collection PubMed
description Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC—HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals).
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spelling pubmed-99846802023-03-05 Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants Gutiérrez-Martín, Daniel Marquès, Montse Pons-Escoda, Albert Vidal, Noemi Bruna, Jordi Restrepo-Montes, Esteban López-Serna, Rebeca García-Sayago, Francisco Majos, Carles Gago-Ferrero, Pablo Gil-Solsona, Rubén MethodsX Environmental Science Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC—HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals). Elsevier 2023-02-10 /pmc/articles/PMC9984680/ /pubmed/36879761 http://dx.doi.org/10.1016/j.mex.2023.102069 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Environmental Science
Gutiérrez-Martín, Daniel
Marquès, Montse
Pons-Escoda, Albert
Vidal, Noemi
Bruna, Jordi
Restrepo-Montes, Esteban
López-Serna, Rebeca
García-Sayago, Francisco
Majos, Carles
Gago-Ferrero, Pablo
Gil-Solsona, Rubén
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants
title Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants
title_full Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants
title_fullStr Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants
title_full_unstemmed Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants
title_short Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants
title_sort tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants
topic Environmental Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9984680/
https://www.ncbi.nlm.nih.gov/pubmed/36879761
http://dx.doi.org/10.1016/j.mex.2023.102069
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