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Heritable Cas9-induced nonhomologous recombination in C. elegans

Identification of the genetic basis of phenotypic variation within species remains challenging. In species with low recombination rates, such as Caenorhabditis elegans , genomic regions linked to a phenotype of interest by genetic mapping studies are often large, making it difficult to identify the...

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Autores principales: Zdraljevic, Stefan, Walter-McNeill, Laura, Marquez, Heriberto, Kruglyak, Leonid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9984945/
https://www.ncbi.nlm.nih.gov/pubmed/36879982
http://dx.doi.org/10.17912/micropub.biology.000775
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author Zdraljevic, Stefan
Walter-McNeill, Laura
Marquez, Heriberto
Kruglyak, Leonid
author_facet Zdraljevic, Stefan
Walter-McNeill, Laura
Marquez, Heriberto
Kruglyak, Leonid
author_sort Zdraljevic, Stefan
collection PubMed
description Identification of the genetic basis of phenotypic variation within species remains challenging. In species with low recombination rates, such as Caenorhabditis elegans , genomic regions linked to a phenotype of interest by genetic mapping studies are often large, making it difficult to identify the specific genes and DNA sequence variants that underlie phenotypic differences. Here, we introduce a method that enables researchers to induce heritable targeted recombination in C. elegans with Cas9. We demonstrate that high rates of targeted nonhomologous recombination can be induced by Cas9 in a genomic region in which naturally occurring meiotic recombination events are exceedingly rare. We anticipate that Cas9-induced nonhomologous recombination (CINR) will greatly facilitate high-resolution genetic mapping in this species.
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spelling pubmed-99849452023-03-05 Heritable Cas9-induced nonhomologous recombination in C. elegans Zdraljevic, Stefan Walter-McNeill, Laura Marquez, Heriberto Kruglyak, Leonid MicroPubl Biol New Methods Identification of the genetic basis of phenotypic variation within species remains challenging. In species with low recombination rates, such as Caenorhabditis elegans , genomic regions linked to a phenotype of interest by genetic mapping studies are often large, making it difficult to identify the specific genes and DNA sequence variants that underlie phenotypic differences. Here, we introduce a method that enables researchers to induce heritable targeted recombination in C. elegans with Cas9. We demonstrate that high rates of targeted nonhomologous recombination can be induced by Cas9 in a genomic region in which naturally occurring meiotic recombination events are exceedingly rare. We anticipate that Cas9-induced nonhomologous recombination (CINR) will greatly facilitate high-resolution genetic mapping in this species. Caltech Library 2023-02-17 /pmc/articles/PMC9984945/ /pubmed/36879982 http://dx.doi.org/10.17912/micropub.biology.000775 Text en Copyright: © 2023 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Methods
Zdraljevic, Stefan
Walter-McNeill, Laura
Marquez, Heriberto
Kruglyak, Leonid
Heritable Cas9-induced nonhomologous recombination in C. elegans
title Heritable Cas9-induced nonhomologous recombination in C. elegans
title_full Heritable Cas9-induced nonhomologous recombination in C. elegans
title_fullStr Heritable Cas9-induced nonhomologous recombination in C. elegans
title_full_unstemmed Heritable Cas9-induced nonhomologous recombination in C. elegans
title_short Heritable Cas9-induced nonhomologous recombination in C. elegans
title_sort heritable cas9-induced nonhomologous recombination in c. elegans
topic New Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9984945/
https://www.ncbi.nlm.nih.gov/pubmed/36879982
http://dx.doi.org/10.17912/micropub.biology.000775
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