Protocol to assemble metal-phenolic framework nanoparticles based on polyphenol-mediated biomimetic mineralization for wound healing in rats

Here, we present a protocol for controllable biomimetic mineralization at the nano-scale, simulating natural ion-enriched sedimentary mineralization. We describe steps for treatment of metal-organic frameworks with polyphenol-mediated stabilized mineralized precursor solution. We then detail their u...

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Detalles Bibliográficos
Autores principales: Zhan, Xiaolong, Wen, Zhengbo, Chen, Yuxin, Feng, Jiaxin, Liang, Ke, Xue, Liecong, He, Peiying, Chen, Honglin, Zhu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985021/
https://www.ncbi.nlm.nih.gov/pubmed/36861839
http://dx.doi.org/10.1016/j.xpro.2023.102131
Descripción
Sumario:Here, we present a protocol for controllable biomimetic mineralization at the nano-scale, simulating natural ion-enriched sedimentary mineralization. We describe steps for treatment of metal-organic frameworks with polyphenol-mediated stabilized mineralized precursor solution. We then detail their use as templates to assemble metal-phenolic frameworks (MPFs) with mineralized layers. Furthermore, we demonstrate the therapeutic benefits of MPF delivery by hydrogel to the full-thickness skin defect model in rats. For complete details on the use and execution of this protocol, please refer to Zhan et al. (2022).(1)

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