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Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice
Mouse hyperglycemia model and islet function assessment are essential in diabetes research. Here, we provide a protocol to evaluate glucose homeostasis and islet functions in diabetic mice and isolated islets. We describe steps for establishing type 1 and 2 diabetes, glucose tolerance test, insulin...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985025/ https://www.ncbi.nlm.nih.gov/pubmed/36861836 http://dx.doi.org/10.1016/j.xpro.2023.102133 |
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author | Chen, Hao Liu, Jian Shi, Guo-Ping Zhang, Xian |
author_facet | Chen, Hao Liu, Jian Shi, Guo-Ping Zhang, Xian |
author_sort | Chen, Hao |
collection | PubMed |
description | Mouse hyperglycemia model and islet function assessment are essential in diabetes research. Here, we provide a protocol to evaluate glucose homeostasis and islet functions in diabetic mice and isolated islets. We describe steps for establishing type 1 and 2 diabetes, glucose tolerance test, insulin tolerance test, glucose stimulated insulin secretion (GSIS) assay, and histological analysis for islet number and insulin expression in vivo. We then detail islet isolation, islet GSIS, β-cell proliferation, apoptosis, and programming assays ex vivo. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).(1) |
format | Online Article Text |
id | pubmed-9985025 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-99850252023-03-05 Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice Chen, Hao Liu, Jian Shi, Guo-Ping Zhang, Xian STAR Protoc Protocol Mouse hyperglycemia model and islet function assessment are essential in diabetes research. Here, we provide a protocol to evaluate glucose homeostasis and islet functions in diabetic mice and isolated islets. We describe steps for establishing type 1 and 2 diabetes, glucose tolerance test, insulin tolerance test, glucose stimulated insulin secretion (GSIS) assay, and histological analysis for islet number and insulin expression in vivo. We then detail islet isolation, islet GSIS, β-cell proliferation, apoptosis, and programming assays ex vivo. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).(1) Elsevier 2023-02-25 /pmc/articles/PMC9985025/ /pubmed/36861836 http://dx.doi.org/10.1016/j.xpro.2023.102133 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Chen, Hao Liu, Jian Shi, Guo-Ping Zhang, Xian Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
title | Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
title_full | Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
title_fullStr | Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
title_full_unstemmed | Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
title_short | Protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
title_sort | protocol for in vivo and ex vivo assessment of hyperglycemia and islet function in diabetic mice |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985025/ https://www.ncbi.nlm.nih.gov/pubmed/36861836 http://dx.doi.org/10.1016/j.xpro.2023.102133 |
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