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Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression
Increased propensity of bone marrow-derived mesenchymal stem cells (BM-MSCs) toward adipogenic differentiation at the expense of osteogenesis has been implicated in obesity, diabetes, and age-related osteoporosis as well as various hematopoietic disorders. Defining small molecules with role in recti...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985040/ https://www.ncbi.nlm.nih.gov/pubmed/36879811 http://dx.doi.org/10.1016/j.isci.2023.106221 |
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author | Zhang, Xianning Liu, Lulu Liu, Xin Huang, Qian Liu, Lei Liu, Haihui Ren, Saisai Wei, Peng Cheng, Panpan Yao, Mingkang Song, Wenjun Zhang, Hao Chen, Mingtai |
author_facet | Zhang, Xianning Liu, Lulu Liu, Xin Huang, Qian Liu, Lei Liu, Haihui Ren, Saisai Wei, Peng Cheng, Panpan Yao, Mingkang Song, Wenjun Zhang, Hao Chen, Mingtai |
author_sort | Zhang, Xianning |
collection | PubMed |
description | Increased propensity of bone marrow-derived mesenchymal stem cells (BM-MSCs) toward adipogenic differentiation at the expense of osteogenesis has been implicated in obesity, diabetes, and age-related osteoporosis as well as various hematopoietic disorders. Defining small molecules with role in rectifying the adipo-osteogenic differentiation imbalance is of great significance. Here, we unexpectedly found that Chidamide, a selective histone deacetylases inhibitor, exhibited remarkably suppressive effect on the in vitro induced adipogenic differentiation of BM-MSCs. Multifaceted alterations in the spectrum of gene expression were observed in Chidamide-managed BM-MSCs during adipogenic induction. Finally, we focused on REEP2, which presented decreased expression in BM-MSCs-mediated adipogenesis and was restored by Chidamide treatment. REEP2 was subsequently demonstrated as a negative regulator of adipogenic differentiation of BM-MSCs and mediated the suppressive effect of Chidamide on adipocyte development. Our findings provide the theoretical and experimental foundation for the clinical application of Chidamide for disorders associated with excessive marrow adipocytes. |
format | Online Article Text |
id | pubmed-9985040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-99850402023-03-05 Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression Zhang, Xianning Liu, Lulu Liu, Xin Huang, Qian Liu, Lei Liu, Haihui Ren, Saisai Wei, Peng Cheng, Panpan Yao, Mingkang Song, Wenjun Zhang, Hao Chen, Mingtai iScience Article Increased propensity of bone marrow-derived mesenchymal stem cells (BM-MSCs) toward adipogenic differentiation at the expense of osteogenesis has been implicated in obesity, diabetes, and age-related osteoporosis as well as various hematopoietic disorders. Defining small molecules with role in rectifying the adipo-osteogenic differentiation imbalance is of great significance. Here, we unexpectedly found that Chidamide, a selective histone deacetylases inhibitor, exhibited remarkably suppressive effect on the in vitro induced adipogenic differentiation of BM-MSCs. Multifaceted alterations in the spectrum of gene expression were observed in Chidamide-managed BM-MSCs during adipogenic induction. Finally, we focused on REEP2, which presented decreased expression in BM-MSCs-mediated adipogenesis and was restored by Chidamide treatment. REEP2 was subsequently demonstrated as a negative regulator of adipogenic differentiation of BM-MSCs and mediated the suppressive effect of Chidamide on adipocyte development. Our findings provide the theoretical and experimental foundation for the clinical application of Chidamide for disorders associated with excessive marrow adipocytes. Elsevier 2023-02-16 /pmc/articles/PMC9985040/ /pubmed/36879811 http://dx.doi.org/10.1016/j.isci.2023.106221 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Zhang, Xianning Liu, Lulu Liu, Xin Huang, Qian Liu, Lei Liu, Haihui Ren, Saisai Wei, Peng Cheng, Panpan Yao, Mingkang Song, Wenjun Zhang, Hao Chen, Mingtai Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression |
title | Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression |
title_full | Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression |
title_fullStr | Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression |
title_full_unstemmed | Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression |
title_short | Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression |
title_sort | chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing reep2 expression |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985040/ https://www.ncbi.nlm.nih.gov/pubmed/36879811 http://dx.doi.org/10.1016/j.isci.2023.106221 |
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