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Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression

Increased propensity of bone marrow-derived mesenchymal stem cells (BM-MSCs) toward adipogenic differentiation at the expense of osteogenesis has been implicated in obesity, diabetes, and age-related osteoporosis as well as various hematopoietic disorders. Defining small molecules with role in recti...

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Autores principales: Zhang, Xianning, Liu, Lulu, Liu, Xin, Huang, Qian, Liu, Lei, Liu, Haihui, Ren, Saisai, Wei, Peng, Cheng, Panpan, Yao, Mingkang, Song, Wenjun, Zhang, Hao, Chen, Mingtai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985040/
https://www.ncbi.nlm.nih.gov/pubmed/36879811
http://dx.doi.org/10.1016/j.isci.2023.106221
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author Zhang, Xianning
Liu, Lulu
Liu, Xin
Huang, Qian
Liu, Lei
Liu, Haihui
Ren, Saisai
Wei, Peng
Cheng, Panpan
Yao, Mingkang
Song, Wenjun
Zhang, Hao
Chen, Mingtai
author_facet Zhang, Xianning
Liu, Lulu
Liu, Xin
Huang, Qian
Liu, Lei
Liu, Haihui
Ren, Saisai
Wei, Peng
Cheng, Panpan
Yao, Mingkang
Song, Wenjun
Zhang, Hao
Chen, Mingtai
author_sort Zhang, Xianning
collection PubMed
description Increased propensity of bone marrow-derived mesenchymal stem cells (BM-MSCs) toward adipogenic differentiation at the expense of osteogenesis has been implicated in obesity, diabetes, and age-related osteoporosis as well as various hematopoietic disorders. Defining small molecules with role in rectifying the adipo-osteogenic differentiation imbalance is of great significance. Here, we unexpectedly found that Chidamide, a selective histone deacetylases inhibitor, exhibited remarkably suppressive effect on the in vitro induced adipogenic differentiation of BM-MSCs. Multifaceted alterations in the spectrum of gene expression were observed in Chidamide-managed BM-MSCs during adipogenic induction. Finally, we focused on REEP2, which presented decreased expression in BM-MSCs-mediated adipogenesis and was restored by Chidamide treatment. REEP2 was subsequently demonstrated as a negative regulator of adipogenic differentiation of BM-MSCs and mediated the suppressive effect of Chidamide on adipocyte development. Our findings provide the theoretical and experimental foundation for the clinical application of Chidamide for disorders associated with excessive marrow adipocytes.
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spelling pubmed-99850402023-03-05 Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression Zhang, Xianning Liu, Lulu Liu, Xin Huang, Qian Liu, Lei Liu, Haihui Ren, Saisai Wei, Peng Cheng, Panpan Yao, Mingkang Song, Wenjun Zhang, Hao Chen, Mingtai iScience Article Increased propensity of bone marrow-derived mesenchymal stem cells (BM-MSCs) toward adipogenic differentiation at the expense of osteogenesis has been implicated in obesity, diabetes, and age-related osteoporosis as well as various hematopoietic disorders. Defining small molecules with role in rectifying the adipo-osteogenic differentiation imbalance is of great significance. Here, we unexpectedly found that Chidamide, a selective histone deacetylases inhibitor, exhibited remarkably suppressive effect on the in vitro induced adipogenic differentiation of BM-MSCs. Multifaceted alterations in the spectrum of gene expression were observed in Chidamide-managed BM-MSCs during adipogenic induction. Finally, we focused on REEP2, which presented decreased expression in BM-MSCs-mediated adipogenesis and was restored by Chidamide treatment. REEP2 was subsequently demonstrated as a negative regulator of adipogenic differentiation of BM-MSCs and mediated the suppressive effect of Chidamide on adipocyte development. Our findings provide the theoretical and experimental foundation for the clinical application of Chidamide for disorders associated with excessive marrow adipocytes. Elsevier 2023-02-16 /pmc/articles/PMC9985040/ /pubmed/36879811 http://dx.doi.org/10.1016/j.isci.2023.106221 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Zhang, Xianning
Liu, Lulu
Liu, Xin
Huang, Qian
Liu, Lei
Liu, Haihui
Ren, Saisai
Wei, Peng
Cheng, Panpan
Yao, Mingkang
Song, Wenjun
Zhang, Hao
Chen, Mingtai
Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression
title Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression
title_full Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression
title_fullStr Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression
title_full_unstemmed Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression
title_short Chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing REEP2 expression
title_sort chidamide suppresses adipogenic differentiation of bone marrow derived mesenchymal stem cells via increasing reep2 expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985040/
https://www.ncbi.nlm.nih.gov/pubmed/36879811
http://dx.doi.org/10.1016/j.isci.2023.106221
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