Cargando…

Matrix Metalloproteinase-2 (MMP-2) and-9 (MMP-9) Gene Variants and Microvascular Complications in Type 2 Diabetes Patients

Vascular complications are the leading cause of increased morbidity and mortality of diabetic patients. It has been postulated that matrix metalloproteinases MMP-2 and MMP-9, zinc-dependent endopeptidases through remodeling of the extracellular matrix, can contribute to the onset and progression of...

Descripción completa

Detalles Bibliográficos
Autores principales: Andjelic, Jelic M, Radojkovic, D, Nikolic, A, Rakicevic, Lj, Babic, T, Jelic, D, Lalic, NM
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985363/
https://www.ncbi.nlm.nih.gov/pubmed/36880042
http://dx.doi.org/10.2478/bjmg-2022-0001
_version_ 1784900936978661376
author Andjelic, Jelic M
Radojkovic, D
Nikolic, A
Rakicevic, Lj
Babic, T
Jelic, D
Lalic, NM
author_facet Andjelic, Jelic M
Radojkovic, D
Nikolic, A
Rakicevic, Lj
Babic, T
Jelic, D
Lalic, NM
author_sort Andjelic, Jelic M
collection PubMed
description Vascular complications are the leading cause of increased morbidity and mortality of diabetic patients. It has been postulated that matrix metalloproteinases MMP-2 and MMP-9, zinc-dependent endopeptidases through remodeling of the extracellular matrix, can contribute to the onset and progression of diabetic vascular complications. The aim of our study was to assess whether there is a major difference in single nucleotide polymorphisms in the MMP-2 (at position -1306C˃T) and MMP-9 (at position -1562C˃T) gene in type 2 diabetic patients and healthy controls and to determine whether there is an association of these gene variants with the presence of microvascular complications in diabetic patients. Our study included 102 type 2 diabetes patients and a control group which was comprised of 56 healthy controls. All diabetic patients were screened for microvascular diabetes complications. Genotypes were detected by polymerase chain reactions followed by restriction analyses with specific endonucleases and their frequencies were determined. The MMP-2 variant -1306C>T showed a negative correlation with type 2 diabetes (p=0.028). It was also shown that the presence of the -1306C allele increases the probability of developing type 2 diabetes. This was a 2.2 fold increase and that the -1306 T allele has a protective role in regards to type 2 diabetes. The MMP-2 variant -1306T showed a negative correlation with diabetic polyneuropathy (p=0.017), meaning that allele-1306T has a protective role in regards to diabetic polyneuropathy while the presence of allele -1306C increases the probability of developing diabetic polyneuropathy by 3.4 fold. Our study showed that the MMP-2 gene variant (-1306C) doubles the risk of developing type 2 diabetes, and for the first time an association of this gene variant and the presence of diabetic polyneuropathy was shown.
format Online
Article
Text
id pubmed-9985363
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Sciendo
record_format MEDLINE/PubMed
spelling pubmed-99853632023-03-05 Matrix Metalloproteinase-2 (MMP-2) and-9 (MMP-9) Gene Variants and Microvascular Complications in Type 2 Diabetes Patients Andjelic, Jelic M Radojkovic, D Nikolic, A Rakicevic, Lj Babic, T Jelic, D Lalic, NM Balkan J Med Genet Original Article Vascular complications are the leading cause of increased morbidity and mortality of diabetic patients. It has been postulated that matrix metalloproteinases MMP-2 and MMP-9, zinc-dependent endopeptidases through remodeling of the extracellular matrix, can contribute to the onset and progression of diabetic vascular complications. The aim of our study was to assess whether there is a major difference in single nucleotide polymorphisms in the MMP-2 (at position -1306C˃T) and MMP-9 (at position -1562C˃T) gene in type 2 diabetic patients and healthy controls and to determine whether there is an association of these gene variants with the presence of microvascular complications in diabetic patients. Our study included 102 type 2 diabetes patients and a control group which was comprised of 56 healthy controls. All diabetic patients were screened for microvascular diabetes complications. Genotypes were detected by polymerase chain reactions followed by restriction analyses with specific endonucleases and their frequencies were determined. The MMP-2 variant -1306C>T showed a negative correlation with type 2 diabetes (p=0.028). It was also shown that the presence of the -1306C allele increases the probability of developing type 2 diabetes. This was a 2.2 fold increase and that the -1306 T allele has a protective role in regards to type 2 diabetes. The MMP-2 variant -1306T showed a negative correlation with diabetic polyneuropathy (p=0.017), meaning that allele-1306T has a protective role in regards to diabetic polyneuropathy while the presence of allele -1306C increases the probability of developing diabetic polyneuropathy by 3.4 fold. Our study showed that the MMP-2 gene variant (-1306C) doubles the risk of developing type 2 diabetes, and for the first time an association of this gene variant and the presence of diabetic polyneuropathy was shown. Sciendo 2023-03-01 /pmc/articles/PMC9985363/ /pubmed/36880042 http://dx.doi.org/10.2478/bjmg-2022-0001 Text en © 2022 Andjelic Jelic M, Radojkovic D, Nikolic A, Rakicevic Lj, Babic T, Jelic D, Lalic NM, published by Sciendo https://creativecommons.org/licenses/by-nc-nd/3.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.
spellingShingle Original Article
Andjelic, Jelic M
Radojkovic, D
Nikolic, A
Rakicevic, Lj
Babic, T
Jelic, D
Lalic, NM
Matrix Metalloproteinase-2 (MMP-2) and-9 (MMP-9) Gene Variants and Microvascular Complications in Type 2 Diabetes Patients
title Matrix Metalloproteinase-2 (MMP-2) and-9 (MMP-9) Gene Variants and Microvascular Complications in Type 2 Diabetes Patients
title_full Matrix Metalloproteinase-2 (MMP-2) and-9 (MMP-9) Gene Variants and Microvascular Complications in Type 2 Diabetes Patients
title_fullStr Matrix Metalloproteinase-2 (MMP-2) and-9 (MMP-9) Gene Variants and Microvascular Complications in Type 2 Diabetes Patients
title_full_unstemmed Matrix Metalloproteinase-2 (MMP-2) and-9 (MMP-9) Gene Variants and Microvascular Complications in Type 2 Diabetes Patients
title_short Matrix Metalloproteinase-2 (MMP-2) and-9 (MMP-9) Gene Variants and Microvascular Complications in Type 2 Diabetes Patients
title_sort matrix metalloproteinase-2 (mmp-2) and-9 (mmp-9) gene variants and microvascular complications in type 2 diabetes patients
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985363/
https://www.ncbi.nlm.nih.gov/pubmed/36880042
http://dx.doi.org/10.2478/bjmg-2022-0001
work_keys_str_mv AT andjelicjelicm matrixmetalloproteinase2mmp2and9mmp9genevariantsandmicrovascularcomplicationsintype2diabetespatients
AT radojkovicd matrixmetalloproteinase2mmp2and9mmp9genevariantsandmicrovascularcomplicationsintype2diabetespatients
AT nikolica matrixmetalloproteinase2mmp2and9mmp9genevariantsandmicrovascularcomplicationsintype2diabetespatients
AT rakiceviclj matrixmetalloproteinase2mmp2and9mmp9genevariantsandmicrovascularcomplicationsintype2diabetespatients
AT babict matrixmetalloproteinase2mmp2and9mmp9genevariantsandmicrovascularcomplicationsintype2diabetespatients
AT jelicd matrixmetalloproteinase2mmp2and9mmp9genevariantsandmicrovascularcomplicationsintype2diabetespatients
AT lalicnm matrixmetalloproteinase2mmp2and9mmp9genevariantsandmicrovascularcomplicationsintype2diabetespatients