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Effect of Endodontic Irrigants and Medicaments Mixed with Silver Nanoparticles against Biofilm Formation of Enterococcus faecalis

INTRODUCTION: The aim of this study was to evaluate the effectiveness of chlorhexidine (CHX), sodium hypochlorite (NaOCl), calcium hydroxide (CH) and double antibiotic paste (DAP) mixed with silver nanoparticles (AgNPs) against Enterococcus faecalis. METHODS AND MATERIALS: Minimum inhibitory concent...

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Autores principales: Ghahramani, Yasamin, Yaghoobi, Fariba, Motamedi, Ramin, Jamshidzadeh, Akram, Abbaszadegan, Abbas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iranian Center for Endodontic Research 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985693/
https://www.ncbi.nlm.nih.gov/pubmed/36883019
http://dx.doi.org/10.22037/iej.v13i4.21843
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author Ghahramani, Yasamin
Yaghoobi, Fariba
Motamedi, Ramin
Jamshidzadeh, Akram
Abbaszadegan, Abbas
author_facet Ghahramani, Yasamin
Yaghoobi, Fariba
Motamedi, Ramin
Jamshidzadeh, Akram
Abbaszadegan, Abbas
author_sort Ghahramani, Yasamin
collection PubMed
description INTRODUCTION: The aim of this study was to evaluate the effectiveness of chlorhexidine (CHX), sodium hypochlorite (NaOCl), calcium hydroxide (CH) and double antibiotic paste (DAP) mixed with silver nanoparticles (AgNPs) against Enterococcus faecalis. METHODS AND MATERIALS: Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and biofilm formation inhibition (after 72 h) of the experimental substances alone or mixed with AgNPs were measured against E. faecalis using microtiter plate method. Bacterial cultures turbidity was measured using a spectrophotometer. All procedures were performed in triplicates. RESULTS: The MIC values for CHX, NaOCl, CH and DAP were equal to 0.012, 1.25, 1.6 and 0.156 mg/mL, and their MBC’s were 0.025, 2.5, 0 and 0.625 mg/mL. After mixing them with AgNPs, the MIC’s for CHX, NaOCl, CH and DAP were reduced to 0.0032, 0.158, 0.2 and 0.0391 mg/mL, while their MBC’s were reduced to 0.0064, 0.0632, 0.401 and 0.0156 mg/mL. Biofilm formation inhibition occurred in higher dilutions of all irrigants and medicaments as they were mixed with Ag NPs. CONCLUSIONS: Adding AgNPs resulted in an increased antimicrobial activity at the tested dilutions for all experimental substances. More investigations in in vivo conditions are required to confirm the results of this study.
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spelling pubmed-99856932023-03-06 Effect of Endodontic Irrigants and Medicaments Mixed with Silver Nanoparticles against Biofilm Formation of Enterococcus faecalis Ghahramani, Yasamin Yaghoobi, Fariba Motamedi, Ramin Jamshidzadeh, Akram Abbaszadegan, Abbas Iran Endod J Original Article INTRODUCTION: The aim of this study was to evaluate the effectiveness of chlorhexidine (CHX), sodium hypochlorite (NaOCl), calcium hydroxide (CH) and double antibiotic paste (DAP) mixed with silver nanoparticles (AgNPs) against Enterococcus faecalis. METHODS AND MATERIALS: Minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and biofilm formation inhibition (after 72 h) of the experimental substances alone or mixed with AgNPs were measured against E. faecalis using microtiter plate method. Bacterial cultures turbidity was measured using a spectrophotometer. All procedures were performed in triplicates. RESULTS: The MIC values for CHX, NaOCl, CH and DAP were equal to 0.012, 1.25, 1.6 and 0.156 mg/mL, and their MBC’s were 0.025, 2.5, 0 and 0.625 mg/mL. After mixing them with AgNPs, the MIC’s for CHX, NaOCl, CH and DAP were reduced to 0.0032, 0.158, 0.2 and 0.0391 mg/mL, while their MBC’s were reduced to 0.0064, 0.0632, 0.401 and 0.0156 mg/mL. Biofilm formation inhibition occurred in higher dilutions of all irrigants and medicaments as they were mixed with Ag NPs. CONCLUSIONS: Adding AgNPs resulted in an increased antimicrobial activity at the tested dilutions for all experimental substances. More investigations in in vivo conditions are required to confirm the results of this study. Iranian Center for Endodontic Research 2018 /pmc/articles/PMC9985693/ /pubmed/36883019 http://dx.doi.org/10.22037/iej.v13i4.21843 Text en © The Author(s). https://creativecommons.org/licenses/by-nc-sa/4.0/This work is licensed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License (https://creativecommons.org/licenses/by-nc-sa/4.0/)
spellingShingle Original Article
Ghahramani, Yasamin
Yaghoobi, Fariba
Motamedi, Ramin
Jamshidzadeh, Akram
Abbaszadegan, Abbas
Effect of Endodontic Irrigants and Medicaments Mixed with Silver Nanoparticles against Biofilm Formation of Enterococcus faecalis
title Effect of Endodontic Irrigants and Medicaments Mixed with Silver Nanoparticles against Biofilm Formation of Enterococcus faecalis
title_full Effect of Endodontic Irrigants and Medicaments Mixed with Silver Nanoparticles against Biofilm Formation of Enterococcus faecalis
title_fullStr Effect of Endodontic Irrigants and Medicaments Mixed with Silver Nanoparticles against Biofilm Formation of Enterococcus faecalis
title_full_unstemmed Effect of Endodontic Irrigants and Medicaments Mixed with Silver Nanoparticles against Biofilm Formation of Enterococcus faecalis
title_short Effect of Endodontic Irrigants and Medicaments Mixed with Silver Nanoparticles against Biofilm Formation of Enterococcus faecalis
title_sort effect of endodontic irrigants and medicaments mixed with silver nanoparticles against biofilm formation of enterococcus faecalis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9985693/
https://www.ncbi.nlm.nih.gov/pubmed/36883019
http://dx.doi.org/10.22037/iej.v13i4.21843
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