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Protective Effect of Silymarin on Liver in Experimental in the Sepsis Model of Rats
This study, it was investigated whether silymarin has a protective effect by performing histological, immunohistochemical, and biochemical evaluations on the liver damage induced by cecal ligation perforation (CLP). CLP model was established and silymarin was treated at a dose of 50 mg/kg, 100 mg/kg...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986308/ https://www.ncbi.nlm.nih.gov/pubmed/36890848 http://dx.doi.org/10.1267/ahc.22-00059 |
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author | Aydemir Celep, Nevra Gedikli, Semin |
author_facet | Aydemir Celep, Nevra Gedikli, Semin |
author_sort | Aydemir Celep, Nevra |
collection | PubMed |
description | This study, it was investigated whether silymarin has a protective effect by performing histological, immunohistochemical, and biochemical evaluations on the liver damage induced by cecal ligation perforation (CLP). CLP model was established and silymarin was treated at a dose of 50 mg/kg, 100 mg/kg, and 200 mg/kg, by oral one hour before the CLP. As an effect of the histological evaluations of the liver tissues, venous congestion, inflammation, and necrosis in the hepatocytes were observed in the CLP group. A situation close to the control group was observed in the Silymarin (SM)100 and SM200 groups. As a result of the immunohistochemical evaluations, inducible nitric oxide synthase (iNOS), cytokeratine (CK)18, Tumor necrosis factor-alpha (TNF-α), and interleukine (IL)-6 immunoreactivities were intense in the CLP group. In the biochemical analysis, Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), and Alanine Aminotransferase (ALT) levels were significantly increased in the CLP group, while a significant decrease was observed in the treatment groups. TNFα, IL-1β, and IL-6 concentrations were in parallel with histopathological evaluations. In the biochemical analysis, Malondialdehyte (MDA) level increased significantly in the CLP group, but there was a significant decrease in the SM100 and SM200 groups. Glutathione (GSH), Superoxide Dismutase (SOD), Catalase (CAT), and Glutathione Peroxidase (GSH-Px) activities were relatively low in the CLP group. According to these data, it was concluded that using silymarin reduces the existing liver damage in sepsis. |
format | Online Article Text |
id | pubmed-9986308 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY |
record_format | MEDLINE/PubMed |
spelling | pubmed-99863082023-03-07 Protective Effect of Silymarin on Liver in Experimental in the Sepsis Model of Rats Aydemir Celep, Nevra Gedikli, Semin Acta Histochem Cytochem Regular Article This study, it was investigated whether silymarin has a protective effect by performing histological, immunohistochemical, and biochemical evaluations on the liver damage induced by cecal ligation perforation (CLP). CLP model was established and silymarin was treated at a dose of 50 mg/kg, 100 mg/kg, and 200 mg/kg, by oral one hour before the CLP. As an effect of the histological evaluations of the liver tissues, venous congestion, inflammation, and necrosis in the hepatocytes were observed in the CLP group. A situation close to the control group was observed in the Silymarin (SM)100 and SM200 groups. As a result of the immunohistochemical evaluations, inducible nitric oxide synthase (iNOS), cytokeratine (CK)18, Tumor necrosis factor-alpha (TNF-α), and interleukine (IL)-6 immunoreactivities were intense in the CLP group. In the biochemical analysis, Alkaline Phosphatase (ALP), Aspartate Aminotransferase (AST), and Alanine Aminotransferase (ALT) levels were significantly increased in the CLP group, while a significant decrease was observed in the treatment groups. TNFα, IL-1β, and IL-6 concentrations were in parallel with histopathological evaluations. In the biochemical analysis, Malondialdehyte (MDA) level increased significantly in the CLP group, but there was a significant decrease in the SM100 and SM200 groups. Glutathione (GSH), Superoxide Dismutase (SOD), Catalase (CAT), and Glutathione Peroxidase (GSH-Px) activities were relatively low in the CLP group. According to these data, it was concluded that using silymarin reduces the existing liver damage in sepsis. JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY 2023-02-28 2023-02-25 /pmc/articles/PMC9986308/ /pubmed/36890848 http://dx.doi.org/10.1267/ahc.22-00059 Text en 2023 The Japan Society of Histochemistry and Cytochemistry https://creativecommons.org/licenses/by-nc/4.0/This is an open access article distributed under the Creative Commons Attribution-NonCommercial 4.0 International License (CC-BY-NC), which permits use, distribution and reproduction of the articles in any medium provided that the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Regular Article Aydemir Celep, Nevra Gedikli, Semin Protective Effect of Silymarin on Liver in Experimental in the Sepsis Model of Rats |
title | Protective Effect of Silymarin on Liver in Experimental in the Sepsis Model of Rats |
title_full | Protective Effect of Silymarin on Liver in Experimental in the Sepsis Model of Rats |
title_fullStr | Protective Effect of Silymarin on Liver in Experimental in the Sepsis Model of Rats |
title_full_unstemmed | Protective Effect of Silymarin on Liver in Experimental in the Sepsis Model of Rats |
title_short | Protective Effect of Silymarin on Liver in Experimental in the Sepsis Model of Rats |
title_sort | protective effect of silymarin on liver in experimental in the sepsis model of rats |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986308/ https://www.ncbi.nlm.nih.gov/pubmed/36890848 http://dx.doi.org/10.1267/ahc.22-00059 |
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