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Inoculum production of Phytophthora medicaginis can be used to screen for partial resistance in chickpea genotypes

Phytophthora root rot caused by Phytophthora medicaginis is an important disease of chickpeas (Cicer arietinum) in Australia with limited management options, increasing reliance on breeding for improved levels of genetic resistance. Resistance based on chickpea–Cicer echinospermum crosses is partial...

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Autores principales: Bithell, Sean L., Drenth, Andre, Backhouse, David, Harden, Steve, Hobson, Kristy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986325/
https://www.ncbi.nlm.nih.gov/pubmed/36890901
http://dx.doi.org/10.3389/fpls.2023.1115417
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author Bithell, Sean L.
Drenth, Andre
Backhouse, David
Harden, Steve
Hobson, Kristy
author_facet Bithell, Sean L.
Drenth, Andre
Backhouse, David
Harden, Steve
Hobson, Kristy
author_sort Bithell, Sean L.
collection PubMed
description Phytophthora root rot caused by Phytophthora medicaginis is an important disease of chickpeas (Cicer arietinum) in Australia with limited management options, increasing reliance on breeding for improved levels of genetic resistance. Resistance based on chickpea–Cicer echinospermum crosses is partial with a quantitative genetic basis provided by C. echinospermum and some disease tolerance traits originating from C. arietinum germplasm. Partial resistance is hypothesised to reduce pathogen proliferation, while tolerant germplasm may contribute some fitness traits, such as an ability to maintain yield despite pathogen proliferation. To test these hypotheses, we used P. medicaginis DNA concentrations in the soil as a parameter for pathogen proliferation and disease assessments on lines of two recombinant inbred populations of chickpea–C. echinospermum crosses to compare the reactions of selected recombinant inbred lines and parents. Our results showed reduced inoculum production in a C. echinospermum backcross parent relative to the C. arietinum variety Yorker. Recombinant inbred lines with consistently low levels of foliage symptoms had significantly lower levels of soil inoculum compared to lines with high levels of visible foliage symptoms. In a separate experiment, a set of superior recombinant inbred lines with consistently low levels of foliage symptoms was tested for soil inoculum reactions relative to control normalised yield loss. The in-crop P. medicaginis soil inoculum concentrations across genotypes were significantly and positively related to yield loss, indicating a partial resistance-tolerance spectrum. Disease incidence and the rankings for in-crop soil inoculum were correlated strongly to yield loss. These results indicate that soil inoculum reactions may be useful to identify genotypes with high levels of partial resistance.
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spelling pubmed-99863252023-03-07 Inoculum production of Phytophthora medicaginis can be used to screen for partial resistance in chickpea genotypes Bithell, Sean L. Drenth, Andre Backhouse, David Harden, Steve Hobson, Kristy Front Plant Sci Plant Science Phytophthora root rot caused by Phytophthora medicaginis is an important disease of chickpeas (Cicer arietinum) in Australia with limited management options, increasing reliance on breeding for improved levels of genetic resistance. Resistance based on chickpea–Cicer echinospermum crosses is partial with a quantitative genetic basis provided by C. echinospermum and some disease tolerance traits originating from C. arietinum germplasm. Partial resistance is hypothesised to reduce pathogen proliferation, while tolerant germplasm may contribute some fitness traits, such as an ability to maintain yield despite pathogen proliferation. To test these hypotheses, we used P. medicaginis DNA concentrations in the soil as a parameter for pathogen proliferation and disease assessments on lines of two recombinant inbred populations of chickpea–C. echinospermum crosses to compare the reactions of selected recombinant inbred lines and parents. Our results showed reduced inoculum production in a C. echinospermum backcross parent relative to the C. arietinum variety Yorker. Recombinant inbred lines with consistently low levels of foliage symptoms had significantly lower levels of soil inoculum compared to lines with high levels of visible foliage symptoms. In a separate experiment, a set of superior recombinant inbred lines with consistently low levels of foliage symptoms was tested for soil inoculum reactions relative to control normalised yield loss. The in-crop P. medicaginis soil inoculum concentrations across genotypes were significantly and positively related to yield loss, indicating a partial resistance-tolerance spectrum. Disease incidence and the rankings for in-crop soil inoculum were correlated strongly to yield loss. These results indicate that soil inoculum reactions may be useful to identify genotypes with high levels of partial resistance. Frontiers Media S.A. 2023-02-20 /pmc/articles/PMC9986325/ /pubmed/36890901 http://dx.doi.org/10.3389/fpls.2023.1115417 Text en Copyright © 2023 Bithell, Drenth, Backhouse, Harden and Hobson https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bithell, Sean L.
Drenth, Andre
Backhouse, David
Harden, Steve
Hobson, Kristy
Inoculum production of Phytophthora medicaginis can be used to screen for partial resistance in chickpea genotypes
title Inoculum production of Phytophthora medicaginis can be used to screen for partial resistance in chickpea genotypes
title_full Inoculum production of Phytophthora medicaginis can be used to screen for partial resistance in chickpea genotypes
title_fullStr Inoculum production of Phytophthora medicaginis can be used to screen for partial resistance in chickpea genotypes
title_full_unstemmed Inoculum production of Phytophthora medicaginis can be used to screen for partial resistance in chickpea genotypes
title_short Inoculum production of Phytophthora medicaginis can be used to screen for partial resistance in chickpea genotypes
title_sort inoculum production of phytophthora medicaginis can be used to screen for partial resistance in chickpea genotypes
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986325/
https://www.ncbi.nlm.nih.gov/pubmed/36890901
http://dx.doi.org/10.3389/fpls.2023.1115417
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