Three-photon excited fluorescence imaging in neuroscience: From principles to applications

The development of three-photon microscopy (3PM) has greatly expanded the capability of imaging deep within biological tissues, enabling neuroscientists to visualize the structure and activity of neuronal populations with greater depth than two-photon imaging. In this review, we outline the history...

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Detalles Bibliográficos
Autores principales: Xiao, Yujie, Deng, Peng, Zhao, Yaoguang, Yang, Shasha, Li, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Neuroscience
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986337/
https://www.ncbi.nlm.nih.gov/pubmed/36891460
http://dx.doi.org/10.3389/fnins.2023.1085682
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author Xiao, Yujie
Deng, Peng
Zhao, Yaoguang
Yang, Shasha
Li, Bo
author_facet Xiao, Yujie
Deng, Peng
Zhao, Yaoguang
Yang, Shasha
Li, Bo
author_sort Xiao, Yujie
collection PubMed
description The development of three-photon microscopy (3PM) has greatly expanded the capability of imaging deep within biological tissues, enabling neuroscientists to visualize the structure and activity of neuronal populations with greater depth than two-photon imaging. In this review, we outline the history and physical principles of 3PM technology. We cover the current techniques for improving the performance of 3PM. Furthermore, we summarize the imaging applications of 3PM for various brain regions and species. Finally, we discuss the future of 3PM applications for neuroscience.
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spelling pubmed-99863372023-03-07 Three-photon excited fluorescence imaging in neuroscience: From principles to applications Xiao, Yujie Deng, Peng Zhao, Yaoguang Yang, Shasha Li, Bo Front Neurosci Neuroscience The development of three-photon microscopy (3PM) has greatly expanded the capability of imaging deep within biological tissues, enabling neuroscientists to visualize the structure and activity of neuronal populations with greater depth than two-photon imaging. In this review, we outline the history and physical principles of 3PM technology. We cover the current techniques for improving the performance of 3PM. Furthermore, we summarize the imaging applications of 3PM for various brain regions and species. Finally, we discuss the future of 3PM applications for neuroscience. Frontiers Media S.A. 2023-02-20 /pmc/articles/PMC9986337/ /pubmed/36891460 http://dx.doi.org/10.3389/fnins.2023.1085682 Text en Copyright © 2023 Xiao, Deng, Zhao, Yang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Xiao, Yujie
Deng, Peng
Zhao, Yaoguang
Yang, Shasha
Li, Bo
Three-photon excited fluorescence imaging in neuroscience: From principles to applications
title Three-photon excited fluorescence imaging in neuroscience: From principles to applications
title_full Three-photon excited fluorescence imaging in neuroscience: From principles to applications
title_fullStr Three-photon excited fluorescence imaging in neuroscience: From principles to applications
title_full_unstemmed Three-photon excited fluorescence imaging in neuroscience: From principles to applications
title_short Three-photon excited fluorescence imaging in neuroscience: From principles to applications
title_sort three-photon excited fluorescence imaging in neuroscience: from principles to applications
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986337/
https://www.ncbi.nlm.nih.gov/pubmed/36891460
http://dx.doi.org/10.3389/fnins.2023.1085682
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