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Effects of Dlx2 overexpression on the genes associated with the maxillary process in the early mouse embryo
The transcription factor Dlx2 plays an important role in craniomaxillofacial development. Overexpression or null mutations of Dlx2 can lead to craniomaxillofacial malformation in mice. However, the transcriptional regulatory effects of Dlx2 during craniomaxillofacial development remain to be elucida...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986417/ https://www.ncbi.nlm.nih.gov/pubmed/36891149 http://dx.doi.org/10.3389/fgene.2023.1085263 |
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author | Sun, Jian Zhang, Jianfei Bian, Qian Wang, Xudong |
author_facet | Sun, Jian Zhang, Jianfei Bian, Qian Wang, Xudong |
author_sort | Sun, Jian |
collection | PubMed |
description | The transcription factor Dlx2 plays an important role in craniomaxillofacial development. Overexpression or null mutations of Dlx2 can lead to craniomaxillofacial malformation in mice. However, the transcriptional regulatory effects of Dlx2 during craniomaxillofacial development remain to be elucidated. Using a mouse model that stably overexpresses Dlx2 in neural crest cells, we comprehensively characterized the effects of Dlx2 overexpression on the early development of maxillary processes in mice by conducting bulk RNA-Seq, scRNA-Seq and CUT&Tag analyses. Bulk RNA-Seq results showed that the overexpression of Dlx2 resulted in substantial transcriptome changes in E10.5 maxillary prominences, with genes involved in RNA metabolism and neuronal development most significantly affected. The scRNA-Seq analysis suggests that overexpression of Dlx2 did not change the differentiation trajectory of mesenchymal cells during this development process. Rather, it restricted cell proliferation and caused precocious differentiation, which may contribute to the defects in craniomaxillofacial development. Moreover, the CUT&Tag analysis using DLX2 antibody revealed enrichment of MNT and Runx2 motifs at the putative DLX2 binding sites, suggesting they may play critical roles in mediating the transcriptional regulatory effects of Dlx2. Together, these results provide important insights for understanding the transcriptional regulatory network of Dlx2 during craniofacial development. |
format | Online Article Text |
id | pubmed-9986417 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99864172023-03-07 Effects of Dlx2 overexpression on the genes associated with the maxillary process in the early mouse embryo Sun, Jian Zhang, Jianfei Bian, Qian Wang, Xudong Front Genet Genetics The transcription factor Dlx2 plays an important role in craniomaxillofacial development. Overexpression or null mutations of Dlx2 can lead to craniomaxillofacial malformation in mice. However, the transcriptional regulatory effects of Dlx2 during craniomaxillofacial development remain to be elucidated. Using a mouse model that stably overexpresses Dlx2 in neural crest cells, we comprehensively characterized the effects of Dlx2 overexpression on the early development of maxillary processes in mice by conducting bulk RNA-Seq, scRNA-Seq and CUT&Tag analyses. Bulk RNA-Seq results showed that the overexpression of Dlx2 resulted in substantial transcriptome changes in E10.5 maxillary prominences, with genes involved in RNA metabolism and neuronal development most significantly affected. The scRNA-Seq analysis suggests that overexpression of Dlx2 did not change the differentiation trajectory of mesenchymal cells during this development process. Rather, it restricted cell proliferation and caused precocious differentiation, which may contribute to the defects in craniomaxillofacial development. Moreover, the CUT&Tag analysis using DLX2 antibody revealed enrichment of MNT and Runx2 motifs at the putative DLX2 binding sites, suggesting they may play critical roles in mediating the transcriptional regulatory effects of Dlx2. Together, these results provide important insights for understanding the transcriptional regulatory network of Dlx2 during craniofacial development. Frontiers Media S.A. 2023-02-20 /pmc/articles/PMC9986417/ /pubmed/36891149 http://dx.doi.org/10.3389/fgene.2023.1085263 Text en Copyright © 2023 Sun, Zhang, Bian and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Genetics Sun, Jian Zhang, Jianfei Bian, Qian Wang, Xudong Effects of Dlx2 overexpression on the genes associated with the maxillary process in the early mouse embryo |
title | Effects of Dlx2 overexpression on the genes associated with the maxillary process in the early mouse embryo |
title_full | Effects of Dlx2 overexpression on the genes associated with the maxillary process in the early mouse embryo |
title_fullStr | Effects of Dlx2 overexpression on the genes associated with the maxillary process in the early mouse embryo |
title_full_unstemmed | Effects of Dlx2 overexpression on the genes associated with the maxillary process in the early mouse embryo |
title_short | Effects of Dlx2 overexpression on the genes associated with the maxillary process in the early mouse embryo |
title_sort | effects of dlx2 overexpression on the genes associated with the maxillary process in the early mouse embryo |
topic | Genetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986417/ https://www.ncbi.nlm.nih.gov/pubmed/36891149 http://dx.doi.org/10.3389/fgene.2023.1085263 |
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