Cargando…

Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype

Forests are at increasing risk from pathogen outbreak. Climate change for example enhance the risk of local disease outbreaks, and naturalization of exotic pathogens may follow human activities, warranting robust pest surveillance routines to support forest management. Melampsora pinitorqua (pine tw...

Descripción completa

Detalles Bibliográficos
Autores principales: Siddique, Abu Bakar, Menke, Laura, Dinedurga, Melis, Albrectsen, Benedicte Riber
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986475/
https://www.ncbi.nlm.nih.gov/pubmed/36890907
http://dx.doi.org/10.3389/fpls.2023.1111001
_version_ 1784901175636656128
author Siddique, Abu Bakar
Menke, Laura
Dinedurga, Melis
Albrectsen, Benedicte Riber
author_facet Siddique, Abu Bakar
Menke, Laura
Dinedurga, Melis
Albrectsen, Benedicte Riber
author_sort Siddique, Abu Bakar
collection PubMed
description Forests are at increasing risk from pathogen outbreak. Climate change for example enhance the risk of local disease outbreaks, and naturalization of exotic pathogens may follow human activities, warranting robust pest surveillance routines to support forest management. Melampsora pinitorqua (pine twisting rust) is of concern in Swedish forestry, and here we evaluate the use of visible rust scores (VRS) on its obligate summer host, European aspen (Populus tremula) as a tool for quantification of the pathogen. With use of species-specific primers, we could detect the native rust, but we failed to detect two exotic rusts (M. medusae and M. larici-populina). We found that aspen genotype determined the presence of fungal genetic markers (amplifying the ITS2 region of the fungal rDNA sequence) as well as DNA sequences specific to M. pinitorqua. We correlated VRS with the amount of fungal DNA in the same leaf, and we related the findings to aspen genotype-specific parameters such as the ability to synthesize and store leaf condensed tannins (CT). At the genotype level both positive and negative relationships were observed between CTs, fungal markers, and rust infestations. However, at the population level, foliar CT concentrations correlated negatively with general fungal- and rust-specific marker abundances. Our results, therefore, do not support the use of VRS to assess Melampsora infestation in Aspen. They do, however, suggest that the relationship between European aspen and rust infestation may be characterized as autochthonous in northern Sweden.
format Online
Article
Text
id pubmed-9986475
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-99864752023-03-07 Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype Siddique, Abu Bakar Menke, Laura Dinedurga, Melis Albrectsen, Benedicte Riber Front Plant Sci Plant Science Forests are at increasing risk from pathogen outbreak. Climate change for example enhance the risk of local disease outbreaks, and naturalization of exotic pathogens may follow human activities, warranting robust pest surveillance routines to support forest management. Melampsora pinitorqua (pine twisting rust) is of concern in Swedish forestry, and here we evaluate the use of visible rust scores (VRS) on its obligate summer host, European aspen (Populus tremula) as a tool for quantification of the pathogen. With use of species-specific primers, we could detect the native rust, but we failed to detect two exotic rusts (M. medusae and M. larici-populina). We found that aspen genotype determined the presence of fungal genetic markers (amplifying the ITS2 region of the fungal rDNA sequence) as well as DNA sequences specific to M. pinitorqua. We correlated VRS with the amount of fungal DNA in the same leaf, and we related the findings to aspen genotype-specific parameters such as the ability to synthesize and store leaf condensed tannins (CT). At the genotype level both positive and negative relationships were observed between CTs, fungal markers, and rust infestations. However, at the population level, foliar CT concentrations correlated negatively with general fungal- and rust-specific marker abundances. Our results, therefore, do not support the use of VRS to assess Melampsora infestation in Aspen. They do, however, suggest that the relationship between European aspen and rust infestation may be characterized as autochthonous in northern Sweden. Frontiers Media S.A. 2023-02-20 /pmc/articles/PMC9986475/ /pubmed/36890907 http://dx.doi.org/10.3389/fpls.2023.1111001 Text en Copyright © 2023 Siddique, Menke, Dinedurga and Albrectsen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Siddique, Abu Bakar
Menke, Laura
Dinedurga, Melis
Albrectsen, Benedicte Riber
Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype
title Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype
title_full Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype
title_fullStr Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype
title_full_unstemmed Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype
title_short Molecular studies of rust on European aspen suggest an autochthonous relationship shaped by genotype
title_sort molecular studies of rust on european aspen suggest an autochthonous relationship shaped by genotype
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986475/
https://www.ncbi.nlm.nih.gov/pubmed/36890907
http://dx.doi.org/10.3389/fpls.2023.1111001
work_keys_str_mv AT siddiqueabubakar molecularstudiesofrustoneuropeanaspensuggestanautochthonousrelationshipshapedbygenotype
AT menkelaura molecularstudiesofrustoneuropeanaspensuggestanautochthonousrelationshipshapedbygenotype
AT dinedurgamelis molecularstudiesofrustoneuropeanaspensuggestanautochthonousrelationshipshapedbygenotype
AT albrectsenbenedicteriber molecularstudiesofrustoneuropeanaspensuggestanautochthonousrelationshipshapedbygenotype