Cargando…

Whole genome sequencing and phylogenetic analysis of African swine fever virus detected in a backyard pig in Mongolia, 2019

African swine fever (ASF) is a highly contagious and fatal disease affecting domestic and wild pigs caused by the African swine fever virus (ASFV). Since the first outbreak in China in August 2018, ASF has spread rapidly in Asia. and the first case in Mongolia was confirmed in January 2019. In this...

Descripción completa

Detalles Bibliográficos
Autores principales: Hyeon, Ji-Yeon, Tseren-Ochir, Erdene-Ochir, Lee, Dong-Hun, Nahm, Sang-Soep, Gladue, Douglas P., Borca, Manuel V., Song, Chang-Seon, Risatti, Guillermo R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986476/
https://www.ncbi.nlm.nih.gov/pubmed/36891469
http://dx.doi.org/10.3389/fvets.2023.1094052
_version_ 1784901175895654400
author Hyeon, Ji-Yeon
Tseren-Ochir, Erdene-Ochir
Lee, Dong-Hun
Nahm, Sang-Soep
Gladue, Douglas P.
Borca, Manuel V.
Song, Chang-Seon
Risatti, Guillermo R.
author_facet Hyeon, Ji-Yeon
Tseren-Ochir, Erdene-Ochir
Lee, Dong-Hun
Nahm, Sang-Soep
Gladue, Douglas P.
Borca, Manuel V.
Song, Chang-Seon
Risatti, Guillermo R.
author_sort Hyeon, Ji-Yeon
collection PubMed
description African swine fever (ASF) is a highly contagious and fatal disease affecting domestic and wild pigs caused by the African swine fever virus (ASFV). Since the first outbreak in China in August 2018, ASF has spread rapidly in Asia. and the first case in Mongolia was confirmed in January 2019. In this study, we report the first whole genome sequence of an ASFV (ASFV SS-3/Mongolia/2019) detected from a backyard pig in Mongolia in February 2019 using whole genome sequencing. We analyzed their phylogenetic relationship with other genotype II ASFVs from Eurasia. The ASFV SS-3/Mongolia/2019 belonged to genotype II (p72 and p54), serogroup 8 (CD2v), Tet-10a variant (pB602L), and IGRIII variant (intergenic region between the I73R/I329L genes). A total of five amino acid substitutions were observed in MGF 360-10L, MGF 505-4R, MGF 505-9R, NP419L, and I267L genes compared to the ASFV Georgia 2007/1 virus. ML phylogenetic analysis of the whole genome sequence showed that the virus shares a high nucleotide sequence identity with ASFVs recently identified in Eastern Europe and Asia and clustered with the ASFV/Zabaykali/WB5314/2020|Russia|2020 virus which was identified at the border between the Russian Federation and Mongolia in 2020. Our results suggest that trans boundary spread of ASF occurred through close geographic proximity.
format Online
Article
Text
id pubmed-9986476
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-99864762023-03-07 Whole genome sequencing and phylogenetic analysis of African swine fever virus detected in a backyard pig in Mongolia, 2019 Hyeon, Ji-Yeon Tseren-Ochir, Erdene-Ochir Lee, Dong-Hun Nahm, Sang-Soep Gladue, Douglas P. Borca, Manuel V. Song, Chang-Seon Risatti, Guillermo R. Front Vet Sci Veterinary Science African swine fever (ASF) is a highly contagious and fatal disease affecting domestic and wild pigs caused by the African swine fever virus (ASFV). Since the first outbreak in China in August 2018, ASF has spread rapidly in Asia. and the first case in Mongolia was confirmed in January 2019. In this study, we report the first whole genome sequence of an ASFV (ASFV SS-3/Mongolia/2019) detected from a backyard pig in Mongolia in February 2019 using whole genome sequencing. We analyzed their phylogenetic relationship with other genotype II ASFVs from Eurasia. The ASFV SS-3/Mongolia/2019 belonged to genotype II (p72 and p54), serogroup 8 (CD2v), Tet-10a variant (pB602L), and IGRIII variant (intergenic region between the I73R/I329L genes). A total of five amino acid substitutions were observed in MGF 360-10L, MGF 505-4R, MGF 505-9R, NP419L, and I267L genes compared to the ASFV Georgia 2007/1 virus. ML phylogenetic analysis of the whole genome sequence showed that the virus shares a high nucleotide sequence identity with ASFVs recently identified in Eastern Europe and Asia and clustered with the ASFV/Zabaykali/WB5314/2020|Russia|2020 virus which was identified at the border between the Russian Federation and Mongolia in 2020. Our results suggest that trans boundary spread of ASF occurred through close geographic proximity. Frontiers Media S.A. 2023-02-20 /pmc/articles/PMC9986476/ /pubmed/36891469 http://dx.doi.org/10.3389/fvets.2023.1094052 Text en Copyright © 2023 Hyeon, Tseren-Ochir, Lee, Nahm, Gladue, Borca, Song and Risatti. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Hyeon, Ji-Yeon
Tseren-Ochir, Erdene-Ochir
Lee, Dong-Hun
Nahm, Sang-Soep
Gladue, Douglas P.
Borca, Manuel V.
Song, Chang-Seon
Risatti, Guillermo R.
Whole genome sequencing and phylogenetic analysis of African swine fever virus detected in a backyard pig in Mongolia, 2019
title Whole genome sequencing and phylogenetic analysis of African swine fever virus detected in a backyard pig in Mongolia, 2019
title_full Whole genome sequencing and phylogenetic analysis of African swine fever virus detected in a backyard pig in Mongolia, 2019
title_fullStr Whole genome sequencing and phylogenetic analysis of African swine fever virus detected in a backyard pig in Mongolia, 2019
title_full_unstemmed Whole genome sequencing and phylogenetic analysis of African swine fever virus detected in a backyard pig in Mongolia, 2019
title_short Whole genome sequencing and phylogenetic analysis of African swine fever virus detected in a backyard pig in Mongolia, 2019
title_sort whole genome sequencing and phylogenetic analysis of african swine fever virus detected in a backyard pig in mongolia, 2019
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986476/
https://www.ncbi.nlm.nih.gov/pubmed/36891469
http://dx.doi.org/10.3389/fvets.2023.1094052
work_keys_str_mv AT hyeonjiyeon wholegenomesequencingandphylogeneticanalysisofafricanswinefevervirusdetectedinabackyardpiginmongolia2019
AT tserenochirerdeneochir wholegenomesequencingandphylogeneticanalysisofafricanswinefevervirusdetectedinabackyardpiginmongolia2019
AT leedonghun wholegenomesequencingandphylogeneticanalysisofafricanswinefevervirusdetectedinabackyardpiginmongolia2019
AT nahmsangsoep wholegenomesequencingandphylogeneticanalysisofafricanswinefevervirusdetectedinabackyardpiginmongolia2019
AT gladuedouglasp wholegenomesequencingandphylogeneticanalysisofafricanswinefevervirusdetectedinabackyardpiginmongolia2019
AT borcamanuelv wholegenomesequencingandphylogeneticanalysisofafricanswinefevervirusdetectedinabackyardpiginmongolia2019
AT songchangseon wholegenomesequencingandphylogeneticanalysisofafricanswinefevervirusdetectedinabackyardpiginmongolia2019
AT risattiguillermor wholegenomesequencingandphylogeneticanalysisofafricanswinefevervirusdetectedinabackyardpiginmongolia2019