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A-910823, a squalene-based emulsion adjuvant, induces T follicular helper cells and humoral immune responses via α-tocopherol component

BACKGROUND: Adjuvants are chemical or biological materials that enhance the efficacy of vaccines. A-910823 is a squalene-based emulsion adjuvant used for S-268019-b, a novel vaccine against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that is currently in clinical development. Publis...

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Autores principales: Yoshioka, Yuya, Kobiyama, Kouji, Hayashi, Tomoya, Onishi, Motoyasu, Yanagida, Yosuke, Nakagawa, Takayuki, Hashimoto, Masayuki, Nishinaka, Anri, Hirose, Jun, Asaoka, Yoshiji, Tajiri, Minako, Hayata, Atsushi, Ishida, Satoru, Omoto, Shinya, Nagira, Morio, Ishii, Ken J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986537/
https://www.ncbi.nlm.nih.gov/pubmed/36891311
http://dx.doi.org/10.3389/fimmu.2023.1116238
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author Yoshioka, Yuya
Kobiyama, Kouji
Hayashi, Tomoya
Onishi, Motoyasu
Yanagida, Yosuke
Nakagawa, Takayuki
Hashimoto, Masayuki
Nishinaka, Anri
Hirose, Jun
Asaoka, Yoshiji
Tajiri, Minako
Hayata, Atsushi
Ishida, Satoru
Omoto, Shinya
Nagira, Morio
Ishii, Ken J.
author_facet Yoshioka, Yuya
Kobiyama, Kouji
Hayashi, Tomoya
Onishi, Motoyasu
Yanagida, Yosuke
Nakagawa, Takayuki
Hashimoto, Masayuki
Nishinaka, Anri
Hirose, Jun
Asaoka, Yoshiji
Tajiri, Minako
Hayata, Atsushi
Ishida, Satoru
Omoto, Shinya
Nagira, Morio
Ishii, Ken J.
author_sort Yoshioka, Yuya
collection PubMed
description BACKGROUND: Adjuvants are chemical or biological materials that enhance the efficacy of vaccines. A-910823 is a squalene-based emulsion adjuvant used for S-268019-b, a novel vaccine against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that is currently in clinical development. Published evidence has demonstrated that A-910823 can enhance the induction of neutralizing antibodies against SARS-CoV-2 in humans and animal models. However, the characteristics and mechanisms of the immune responses induced by A-910823 are not yet known. METHODS AND RESULTS: To characterize A-910823, we compared the adaptive immune response profile enhanced by A-910823 with that of other adjuvants (AddaVax, QS21, aluminum salt-based adjuvants, and empty lipid nanoparticle [eLNP]) in a murine model. Compared with other adjuvants, A-910823 enhanced humoral immune responses to an equal or greater extent following potent T follicular helper (Tfh) and germinal center B (GCB) cell induction, without inducing a strong systemic inflammatory cytokine response. Furthermore, S-268019-b containing A-910823 adjuvant produced similar results even when given as a booster dose following primary administration of a lipid nanoparticle-encapsulated messenger RNA (mRNA-LNP) vaccine. Preparation of modified A-910823 adjuvants to identify which components of A-910823 play a role in driving the adjuvant effect and detailed evaluation of the immunological characteristics induced by each adjuvant showed that the induction of humoral immunity and Tfh and GCB cell induction in A-910823 were dependent on α-tocopherol. Finally, we revealed that the recruitment of inflammatory cells to the draining lymph nodes and induction of serum cytokines and chemokines by A-910823 were also dependent on the α-tocopherol component. CONCLUSIONS: This study demonstrates that the novel adjuvant A-910823 is capable of robust Tfh cell induction and humoral immune responses, even when given as a booster dose. The findings also emphasize that α-tocopherol drives the potent Tfh-inducing adjuvant function of A-910823. Overall, our data provide key information that may inform the future production of improved adjuvants.
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spelling pubmed-99865372023-03-07 A-910823, a squalene-based emulsion adjuvant, induces T follicular helper cells and humoral immune responses via α-tocopherol component Yoshioka, Yuya Kobiyama, Kouji Hayashi, Tomoya Onishi, Motoyasu Yanagida, Yosuke Nakagawa, Takayuki Hashimoto, Masayuki Nishinaka, Anri Hirose, Jun Asaoka, Yoshiji Tajiri, Minako Hayata, Atsushi Ishida, Satoru Omoto, Shinya Nagira, Morio Ishii, Ken J. Front Immunol Immunology BACKGROUND: Adjuvants are chemical or biological materials that enhance the efficacy of vaccines. A-910823 is a squalene-based emulsion adjuvant used for S-268019-b, a novel vaccine against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that is currently in clinical development. Published evidence has demonstrated that A-910823 can enhance the induction of neutralizing antibodies against SARS-CoV-2 in humans and animal models. However, the characteristics and mechanisms of the immune responses induced by A-910823 are not yet known. METHODS AND RESULTS: To characterize A-910823, we compared the adaptive immune response profile enhanced by A-910823 with that of other adjuvants (AddaVax, QS21, aluminum salt-based adjuvants, and empty lipid nanoparticle [eLNP]) in a murine model. Compared with other adjuvants, A-910823 enhanced humoral immune responses to an equal or greater extent following potent T follicular helper (Tfh) and germinal center B (GCB) cell induction, without inducing a strong systemic inflammatory cytokine response. Furthermore, S-268019-b containing A-910823 adjuvant produced similar results even when given as a booster dose following primary administration of a lipid nanoparticle-encapsulated messenger RNA (mRNA-LNP) vaccine. Preparation of modified A-910823 adjuvants to identify which components of A-910823 play a role in driving the adjuvant effect and detailed evaluation of the immunological characteristics induced by each adjuvant showed that the induction of humoral immunity and Tfh and GCB cell induction in A-910823 were dependent on α-tocopherol. Finally, we revealed that the recruitment of inflammatory cells to the draining lymph nodes and induction of serum cytokines and chemokines by A-910823 were also dependent on the α-tocopherol component. CONCLUSIONS: This study demonstrates that the novel adjuvant A-910823 is capable of robust Tfh cell induction and humoral immune responses, even when given as a booster dose. The findings also emphasize that α-tocopherol drives the potent Tfh-inducing adjuvant function of A-910823. Overall, our data provide key information that may inform the future production of improved adjuvants. Frontiers Media S.A. 2023-02-20 /pmc/articles/PMC9986537/ /pubmed/36891311 http://dx.doi.org/10.3389/fimmu.2023.1116238 Text en Copyright © 2023 Yoshioka, Kobiyama, Hayashi, Onishi, Yanagida, Nakagawa, Hashimoto, Nishinaka, Hirose, Asaoka, Tajiri, Hayata, Ishida, Omoto, Nagira and Ishii https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Yoshioka, Yuya
Kobiyama, Kouji
Hayashi, Tomoya
Onishi, Motoyasu
Yanagida, Yosuke
Nakagawa, Takayuki
Hashimoto, Masayuki
Nishinaka, Anri
Hirose, Jun
Asaoka, Yoshiji
Tajiri, Minako
Hayata, Atsushi
Ishida, Satoru
Omoto, Shinya
Nagira, Morio
Ishii, Ken J.
A-910823, a squalene-based emulsion adjuvant, induces T follicular helper cells and humoral immune responses via α-tocopherol component
title A-910823, a squalene-based emulsion adjuvant, induces T follicular helper cells and humoral immune responses via α-tocopherol component
title_full A-910823, a squalene-based emulsion adjuvant, induces T follicular helper cells and humoral immune responses via α-tocopherol component
title_fullStr A-910823, a squalene-based emulsion adjuvant, induces T follicular helper cells and humoral immune responses via α-tocopherol component
title_full_unstemmed A-910823, a squalene-based emulsion adjuvant, induces T follicular helper cells and humoral immune responses via α-tocopherol component
title_short A-910823, a squalene-based emulsion adjuvant, induces T follicular helper cells and humoral immune responses via α-tocopherol component
title_sort a-910823, a squalene-based emulsion adjuvant, induces t follicular helper cells and humoral immune responses via α-tocopherol component
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986537/
https://www.ncbi.nlm.nih.gov/pubmed/36891311
http://dx.doi.org/10.3389/fimmu.2023.1116238
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