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Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors
PURPOSE: Modern techniques for improved tumor visualization have the aim to maximize the extent of resection during brain tumor surgery and thus improve patient prognosis. Optical imaging of autofluorescence is a powerful and non-invasive tool to monitor metabolic changes and transformation in brain...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986542/ https://www.ncbi.nlm.nih.gov/pubmed/36890834 http://dx.doi.org/10.3389/fonc.2023.1105648 |
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author | Reichert, David Wadiura, Lisa I. Erkkilae, Mikael T. Gesperger, Johanna Lang, Alexandra Roetzer-Pejrimovsky, Thomas Makolli, Jessica Woehrer, Adelheid Wilzbach, Marco Hauger, Christoph Kiesel, Barbara Andreana, Marco Unterhuber, Angelika Drexler, Wolfgang Widhalm, Georg Leitgeb, Rainer A. |
author_facet | Reichert, David Wadiura, Lisa I. Erkkilae, Mikael T. Gesperger, Johanna Lang, Alexandra Roetzer-Pejrimovsky, Thomas Makolli, Jessica Woehrer, Adelheid Wilzbach, Marco Hauger, Christoph Kiesel, Barbara Andreana, Marco Unterhuber, Angelika Drexler, Wolfgang Widhalm, Georg Leitgeb, Rainer A. |
author_sort | Reichert, David |
collection | PubMed |
description | PURPOSE: Modern techniques for improved tumor visualization have the aim to maximize the extent of resection during brain tumor surgery and thus improve patient prognosis. Optical imaging of autofluorescence is a powerful and non-invasive tool to monitor metabolic changes and transformation in brain tumors. Cellular redox ratios can be retrieved from fluorescence emitted by the coenzymes reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD). Recent studies point out that the influence of flavin mononucleotide (FMN) has been underestimated. EXPERIMENTAL DESIGN: Fluorescence lifetime imaging and fluorescence spectroscopy were performed through a modified surgical microscope. We acquired 361 flavin fluorescence lifetime (500-580 nm) and fluorescence spectra (430-740 nm) data points on freshly excised different brain tumors: low-grade gliomas (N=17), high-grade gliomas (N=42), meningiomas (N=23), metastases (N=26) and specimens from the non-tumorous brain (N=3). RESULTS: Protein-bound FMN fluorescence in brain tumors did increase with a shift toward a more glycolytic metabolism (R=-0.87). This increased the average flavin fluorescence lifetime in tumor entities with respect to the non-tumorous brain. Further, these metrics were characteristic for the different tumor entities and showed promise for machine learning based brain tumor classification. CONCLUSIONS: Our results shed light on FMN fluorescence in metabolic imaging and outline the potential for supporting the neurosurgeon in visualizing and classifying brain tumor tissue during surgery. |
format | Online Article Text |
id | pubmed-9986542 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99865422023-03-07 Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors Reichert, David Wadiura, Lisa I. Erkkilae, Mikael T. Gesperger, Johanna Lang, Alexandra Roetzer-Pejrimovsky, Thomas Makolli, Jessica Woehrer, Adelheid Wilzbach, Marco Hauger, Christoph Kiesel, Barbara Andreana, Marco Unterhuber, Angelika Drexler, Wolfgang Widhalm, Georg Leitgeb, Rainer A. Front Oncol Oncology PURPOSE: Modern techniques for improved tumor visualization have the aim to maximize the extent of resection during brain tumor surgery and thus improve patient prognosis. Optical imaging of autofluorescence is a powerful and non-invasive tool to monitor metabolic changes and transformation in brain tumors. Cellular redox ratios can be retrieved from fluorescence emitted by the coenzymes reduced nicotinamide adenine dinucleotide (phosphate) (NAD(P)H) and flavin adenine dinucleotide (FAD). Recent studies point out that the influence of flavin mononucleotide (FMN) has been underestimated. EXPERIMENTAL DESIGN: Fluorescence lifetime imaging and fluorescence spectroscopy were performed through a modified surgical microscope. We acquired 361 flavin fluorescence lifetime (500-580 nm) and fluorescence spectra (430-740 nm) data points on freshly excised different brain tumors: low-grade gliomas (N=17), high-grade gliomas (N=42), meningiomas (N=23), metastases (N=26) and specimens from the non-tumorous brain (N=3). RESULTS: Protein-bound FMN fluorescence in brain tumors did increase with a shift toward a more glycolytic metabolism (R=-0.87). This increased the average flavin fluorescence lifetime in tumor entities with respect to the non-tumorous brain. Further, these metrics were characteristic for the different tumor entities and showed promise for machine learning based brain tumor classification. CONCLUSIONS: Our results shed light on FMN fluorescence in metabolic imaging and outline the potential for supporting the neurosurgeon in visualizing and classifying brain tumor tissue during surgery. Frontiers Media S.A. 2023-02-20 /pmc/articles/PMC9986542/ /pubmed/36890834 http://dx.doi.org/10.3389/fonc.2023.1105648 Text en Copyright © 2023 Reichert, Wadiura, Erkkilae, Gesperger, Lang, Roetzer-Pejrimovsky, Makolli, Woehrer, Wilzbach, Hauger, Kiesel, Andreana, Unterhuber, Drexler, Widhalm and Leitgeb https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Oncology Reichert, David Wadiura, Lisa I. Erkkilae, Mikael T. Gesperger, Johanna Lang, Alexandra Roetzer-Pejrimovsky, Thomas Makolli, Jessica Woehrer, Adelheid Wilzbach, Marco Hauger, Christoph Kiesel, Barbara Andreana, Marco Unterhuber, Angelika Drexler, Wolfgang Widhalm, Georg Leitgeb, Rainer A. Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
title | Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
title_full | Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
title_fullStr | Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
title_full_unstemmed | Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
title_short | Flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
title_sort | flavin fluorescence lifetime and autofluorescence optical redox ratio for improved visualization and classification of brain tumors |
topic | Oncology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9986542/ https://www.ncbi.nlm.nih.gov/pubmed/36890834 http://dx.doi.org/10.3389/fonc.2023.1105648 |
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