Cargando…

Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept

OBJECTIVE: To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging. APPROACH: Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular...

Descripción completa

Detalles Bibliográficos
Autores principales: Gomez-Gonzalez, Emilio, Muñoz, Olga, Gomez-Martin, Juan Carlos, Aceituno-Castro, Jesus, Fernandez-Muñoz, Beatriz, Navas-Garcia, Jose Manuel, Barriga-Rivera, Alejandro, Fernandez-Lizaranzu, Isabel, Munoz-Gonzalez, Francisco Javier, Parrilla-Giraldez, Ruben, Requena-Lancharro, Desiree, Gil-Gamboa, Pedro, Ramos, José Luis, Rosell-Valle, Cristina, Gomez-Gonzalez, Carmen, Martin-Lopez, Maria, Relimpio-Lopez, Maria Isabel, Perales-Esteve, Manuel A., Puppo-Moreno, Antonio, Garcia-Cozar, Francisco Jose, Olvera-Collantes, Lucia, de los Santos-Trigo, Silvia, Gomez, Emilia, Sanchez-Pernaute, Rosario, Padillo-Ruiz, Javier, Marquez-Rivas, Javier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier Ltd. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9987604/
https://www.ncbi.nlm.nih.gov/pubmed/36945203
http://dx.doi.org/10.1016/j.jqsrt.2023.108567
_version_ 1784901414837813248
author Gomez-Gonzalez, Emilio
Muñoz, Olga
Gomez-Martin, Juan Carlos
Aceituno-Castro, Jesus
Fernandez-Muñoz, Beatriz
Navas-Garcia, Jose Manuel
Barriga-Rivera, Alejandro
Fernandez-Lizaranzu, Isabel
Munoz-Gonzalez, Francisco Javier
Parrilla-Giraldez, Ruben
Requena-Lancharro, Desiree
Gil-Gamboa, Pedro
Ramos, José Luis
Rosell-Valle, Cristina
Gomez-Gonzalez, Carmen
Martin-Lopez, Maria
Relimpio-Lopez, Maria Isabel
Perales-Esteve, Manuel A.
Puppo-Moreno, Antonio
Garcia-Cozar, Francisco Jose
Olvera-Collantes, Lucia
de los Santos-Trigo, Silvia
Gomez, Emilia
Sanchez-Pernaute, Rosario
Padillo-Ruiz, Javier
Marquez-Rivas, Javier
author_facet Gomez-Gonzalez, Emilio
Muñoz, Olga
Gomez-Martin, Juan Carlos
Aceituno-Castro, Jesus
Fernandez-Muñoz, Beatriz
Navas-Garcia, Jose Manuel
Barriga-Rivera, Alejandro
Fernandez-Lizaranzu, Isabel
Munoz-Gonzalez, Francisco Javier
Parrilla-Giraldez, Ruben
Requena-Lancharro, Desiree
Gil-Gamboa, Pedro
Ramos, José Luis
Rosell-Valle, Cristina
Gomez-Gonzalez, Carmen
Martin-Lopez, Maria
Relimpio-Lopez, Maria Isabel
Perales-Esteve, Manuel A.
Puppo-Moreno, Antonio
Garcia-Cozar, Francisco Jose
Olvera-Collantes, Lucia
de los Santos-Trigo, Silvia
Gomez, Emilia
Sanchez-Pernaute, Rosario
Padillo-Ruiz, Javier
Marquez-Rivas, Javier
author_sort Gomez-Gonzalez, Emilio
collection PubMed
description OBJECTIVE: To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging. APPROACH: Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular stomatitis virus, and with the characteristic Spike protein of SARS-CoV-2. Samples were prepared in two biofluids (saline solution and artificial saliva), in four concentrations, and deposited as 5-µL droplets on a supporting plate. The angles of maximal degree of linear polarization (DLP) of light diffusely scattered from dry residues were determined using Mueller polarimetry from87 samples at 405 nm and 514 nm. A polarimetric camera was used for imaging several samples under 380–420 nm illumination at angles similar to those of maximal DLP. Per-pixel image analysis included quantification and combination of polarization feature descriptors in 475 samples. MAIN RESULTS: The angles (from sample surface) of maximal DLP were 3° for 405 nm and 6° for 514 nm. Similar viral particles that differed only in the characteristic spike protein of the SARS-CoV-2, their corresponding negative controls, fluids, and the sample holder were discerned at 10-degree and 15-degree configurations. SIGNIFICANCE: Polarimetric imaging in the visible spectrum may help improve fast, non-contact detection and identification of viral particles, and/or other microbes such as tuberculosis, in multiple dry fluid samples simultaneously, particularly when combined with other imaging modalities. Further analysis including realistic concentrations of real SARS-CoV-2 viral particles in relevant human fluids is required. Polarimetric imaging under visible light may contribute to a fast, cost-effective screening of SARS-CoV-2 and other pathogens when combined with other imaging modalities.
format Online
Article
Text
id pubmed-9987604
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher The Author(s). Published by Elsevier Ltd.
record_format MEDLINE/PubMed
spelling pubmed-99876042023-03-07 Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept Gomez-Gonzalez, Emilio Muñoz, Olga Gomez-Martin, Juan Carlos Aceituno-Castro, Jesus Fernandez-Muñoz, Beatriz Navas-Garcia, Jose Manuel Barriga-Rivera, Alejandro Fernandez-Lizaranzu, Isabel Munoz-Gonzalez, Francisco Javier Parrilla-Giraldez, Ruben Requena-Lancharro, Desiree Gil-Gamboa, Pedro Ramos, José Luis Rosell-Valle, Cristina Gomez-Gonzalez, Carmen Martin-Lopez, Maria Relimpio-Lopez, Maria Isabel Perales-Esteve, Manuel A. Puppo-Moreno, Antonio Garcia-Cozar, Francisco Jose Olvera-Collantes, Lucia de los Santos-Trigo, Silvia Gomez, Emilia Sanchez-Pernaute, Rosario Padillo-Ruiz, Javier Marquez-Rivas, Javier J Quant Spectrosc Radiat Transf Article OBJECTIVE: To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging. APPROACH: Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular stomatitis virus, and with the characteristic Spike protein of SARS-CoV-2. Samples were prepared in two biofluids (saline solution and artificial saliva), in four concentrations, and deposited as 5-µL droplets on a supporting plate. The angles of maximal degree of linear polarization (DLP) of light diffusely scattered from dry residues were determined using Mueller polarimetry from87 samples at 405 nm and 514 nm. A polarimetric camera was used for imaging several samples under 380–420 nm illumination at angles similar to those of maximal DLP. Per-pixel image analysis included quantification and combination of polarization feature descriptors in 475 samples. MAIN RESULTS: The angles (from sample surface) of maximal DLP were 3° for 405 nm and 6° for 514 nm. Similar viral particles that differed only in the characteristic spike protein of the SARS-CoV-2, their corresponding negative controls, fluids, and the sample holder were discerned at 10-degree and 15-degree configurations. SIGNIFICANCE: Polarimetric imaging in the visible spectrum may help improve fast, non-contact detection and identification of viral particles, and/or other microbes such as tuberculosis, in multiple dry fluid samples simultaneously, particularly when combined with other imaging modalities. Further analysis including realistic concentrations of real SARS-CoV-2 viral particles in relevant human fluids is required. Polarimetric imaging under visible light may contribute to a fast, cost-effective screening of SARS-CoV-2 and other pathogens when combined with other imaging modalities. The Author(s). Published by Elsevier Ltd. 2023-07 2023-03-06 /pmc/articles/PMC9987604/ /pubmed/36945203 http://dx.doi.org/10.1016/j.jqsrt.2023.108567 Text en © 2023 The Author(s) Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Gomez-Gonzalez, Emilio
Muñoz, Olga
Gomez-Martin, Juan Carlos
Aceituno-Castro, Jesus
Fernandez-Muñoz, Beatriz
Navas-Garcia, Jose Manuel
Barriga-Rivera, Alejandro
Fernandez-Lizaranzu, Isabel
Munoz-Gonzalez, Francisco Javier
Parrilla-Giraldez, Ruben
Requena-Lancharro, Desiree
Gil-Gamboa, Pedro
Ramos, José Luis
Rosell-Valle, Cristina
Gomez-Gonzalez, Carmen
Martin-Lopez, Maria
Relimpio-Lopez, Maria Isabel
Perales-Esteve, Manuel A.
Puppo-Moreno, Antonio
Garcia-Cozar, Francisco Jose
Olvera-Collantes, Lucia
de los Santos-Trigo, Silvia
Gomez, Emilia
Sanchez-Pernaute, Rosario
Padillo-Ruiz, Javier
Marquez-Rivas, Javier
Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept
title Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept
title_full Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept
title_fullStr Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept
title_full_unstemmed Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept
title_short Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept
title_sort polarimetric imaging for the detection of synthetic models of sars-cov-2: a proof of concept
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9987604/
https://www.ncbi.nlm.nih.gov/pubmed/36945203
http://dx.doi.org/10.1016/j.jqsrt.2023.108567
work_keys_str_mv AT gomezgonzalezemilio polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT munozolga polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT gomezmartinjuancarlos polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT aceitunocastrojesus polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT fernandezmunozbeatriz polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT navasgarciajosemanuel polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT barrigariveraalejandro polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT fernandezlizaranzuisabel polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT munozgonzalezfranciscojavier polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT parrillagiraldezruben polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT requenalancharrodesiree polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT gilgamboapedro polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT ramosjoseluis polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT rosellvallecristina polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT gomezgonzalezcarmen polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT martinlopezmaria polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT relimpiolopezmariaisabel polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT peralesestevemanuela polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT puppomorenoantonio polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT garciacozarfranciscojose polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT olveracollanteslucia polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT delossantostrigosilvia polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT gomezemilia polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT sanchezpernauterosario polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT padilloruizjavier polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept
AT marquezrivasjavier polarimetricimagingforthedetectionofsyntheticmodelsofsarscov2aproofofconcept