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Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept
OBJECTIVE: To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging. APPROACH: Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Author(s). Published by Elsevier Ltd.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9987604/ https://www.ncbi.nlm.nih.gov/pubmed/36945203 http://dx.doi.org/10.1016/j.jqsrt.2023.108567 |
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author | Gomez-Gonzalez, Emilio Muñoz, Olga Gomez-Martin, Juan Carlos Aceituno-Castro, Jesus Fernandez-Muñoz, Beatriz Navas-Garcia, Jose Manuel Barriga-Rivera, Alejandro Fernandez-Lizaranzu, Isabel Munoz-Gonzalez, Francisco Javier Parrilla-Giraldez, Ruben Requena-Lancharro, Desiree Gil-Gamboa, Pedro Ramos, José Luis Rosell-Valle, Cristina Gomez-Gonzalez, Carmen Martin-Lopez, Maria Relimpio-Lopez, Maria Isabel Perales-Esteve, Manuel A. Puppo-Moreno, Antonio Garcia-Cozar, Francisco Jose Olvera-Collantes, Lucia de los Santos-Trigo, Silvia Gomez, Emilia Sanchez-Pernaute, Rosario Padillo-Ruiz, Javier Marquez-Rivas, Javier |
author_facet | Gomez-Gonzalez, Emilio Muñoz, Olga Gomez-Martin, Juan Carlos Aceituno-Castro, Jesus Fernandez-Muñoz, Beatriz Navas-Garcia, Jose Manuel Barriga-Rivera, Alejandro Fernandez-Lizaranzu, Isabel Munoz-Gonzalez, Francisco Javier Parrilla-Giraldez, Ruben Requena-Lancharro, Desiree Gil-Gamboa, Pedro Ramos, José Luis Rosell-Valle, Cristina Gomez-Gonzalez, Carmen Martin-Lopez, Maria Relimpio-Lopez, Maria Isabel Perales-Esteve, Manuel A. Puppo-Moreno, Antonio Garcia-Cozar, Francisco Jose Olvera-Collantes, Lucia de los Santos-Trigo, Silvia Gomez, Emilia Sanchez-Pernaute, Rosario Padillo-Ruiz, Javier Marquez-Rivas, Javier |
author_sort | Gomez-Gonzalez, Emilio |
collection | PubMed |
description | OBJECTIVE: To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging. APPROACH: Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular stomatitis virus, and with the characteristic Spike protein of SARS-CoV-2. Samples were prepared in two biofluids (saline solution and artificial saliva), in four concentrations, and deposited as 5-µL droplets on a supporting plate. The angles of maximal degree of linear polarization (DLP) of light diffusely scattered from dry residues were determined using Mueller polarimetry from87 samples at 405 nm and 514 nm. A polarimetric camera was used for imaging several samples under 380–420 nm illumination at angles similar to those of maximal DLP. Per-pixel image analysis included quantification and combination of polarization feature descriptors in 475 samples. MAIN RESULTS: The angles (from sample surface) of maximal DLP were 3° for 405 nm and 6° for 514 nm. Similar viral particles that differed only in the characteristic spike protein of the SARS-CoV-2, their corresponding negative controls, fluids, and the sample holder were discerned at 10-degree and 15-degree configurations. SIGNIFICANCE: Polarimetric imaging in the visible spectrum may help improve fast, non-contact detection and identification of viral particles, and/or other microbes such as tuberculosis, in multiple dry fluid samples simultaneously, particularly when combined with other imaging modalities. Further analysis including realistic concentrations of real SARS-CoV-2 viral particles in relevant human fluids is required. Polarimetric imaging under visible light may contribute to a fast, cost-effective screening of SARS-CoV-2 and other pathogens when combined with other imaging modalities. |
format | Online Article Text |
id | pubmed-9987604 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Author(s). Published by Elsevier Ltd. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99876042023-03-07 Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept Gomez-Gonzalez, Emilio Muñoz, Olga Gomez-Martin, Juan Carlos Aceituno-Castro, Jesus Fernandez-Muñoz, Beatriz Navas-Garcia, Jose Manuel Barriga-Rivera, Alejandro Fernandez-Lizaranzu, Isabel Munoz-Gonzalez, Francisco Javier Parrilla-Giraldez, Ruben Requena-Lancharro, Desiree Gil-Gamboa, Pedro Ramos, José Luis Rosell-Valle, Cristina Gomez-Gonzalez, Carmen Martin-Lopez, Maria Relimpio-Lopez, Maria Isabel Perales-Esteve, Manuel A. Puppo-Moreno, Antonio Garcia-Cozar, Francisco Jose Olvera-Collantes, Lucia de los Santos-Trigo, Silvia Gomez, Emilia Sanchez-Pernaute, Rosario Padillo-Ruiz, Javier Marquez-Rivas, Javier J Quant Spectrosc Radiat Transf Article OBJECTIVE: To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging. APPROACH: Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular stomatitis virus, and with the characteristic Spike protein of SARS-CoV-2. Samples were prepared in two biofluids (saline solution and artificial saliva), in four concentrations, and deposited as 5-µL droplets on a supporting plate. The angles of maximal degree of linear polarization (DLP) of light diffusely scattered from dry residues were determined using Mueller polarimetry from87 samples at 405 nm and 514 nm. A polarimetric camera was used for imaging several samples under 380–420 nm illumination at angles similar to those of maximal DLP. Per-pixel image analysis included quantification and combination of polarization feature descriptors in 475 samples. MAIN RESULTS: The angles (from sample surface) of maximal DLP were 3° for 405 nm and 6° for 514 nm. Similar viral particles that differed only in the characteristic spike protein of the SARS-CoV-2, their corresponding negative controls, fluids, and the sample holder were discerned at 10-degree and 15-degree configurations. SIGNIFICANCE: Polarimetric imaging in the visible spectrum may help improve fast, non-contact detection and identification of viral particles, and/or other microbes such as tuberculosis, in multiple dry fluid samples simultaneously, particularly when combined with other imaging modalities. Further analysis including realistic concentrations of real SARS-CoV-2 viral particles in relevant human fluids is required. Polarimetric imaging under visible light may contribute to a fast, cost-effective screening of SARS-CoV-2 and other pathogens when combined with other imaging modalities. The Author(s). Published by Elsevier Ltd. 2023-07 2023-03-06 /pmc/articles/PMC9987604/ /pubmed/36945203 http://dx.doi.org/10.1016/j.jqsrt.2023.108567 Text en © 2023 The Author(s) Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active. |
spellingShingle | Article Gomez-Gonzalez, Emilio Muñoz, Olga Gomez-Martin, Juan Carlos Aceituno-Castro, Jesus Fernandez-Muñoz, Beatriz Navas-Garcia, Jose Manuel Barriga-Rivera, Alejandro Fernandez-Lizaranzu, Isabel Munoz-Gonzalez, Francisco Javier Parrilla-Giraldez, Ruben Requena-Lancharro, Desiree Gil-Gamboa, Pedro Ramos, José Luis Rosell-Valle, Cristina Gomez-Gonzalez, Carmen Martin-Lopez, Maria Relimpio-Lopez, Maria Isabel Perales-Esteve, Manuel A. Puppo-Moreno, Antonio Garcia-Cozar, Francisco Jose Olvera-Collantes, Lucia de los Santos-Trigo, Silvia Gomez, Emilia Sanchez-Pernaute, Rosario Padillo-Ruiz, Javier Marquez-Rivas, Javier Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept |
title | Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept |
title_full | Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept |
title_fullStr | Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept |
title_full_unstemmed | Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept |
title_short | Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: A proof of concept |
title_sort | polarimetric imaging for the detection of synthetic models of sars-cov-2: a proof of concept |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9987604/ https://www.ncbi.nlm.nih.gov/pubmed/36945203 http://dx.doi.org/10.1016/j.jqsrt.2023.108567 |
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