Cargando…

EFEMP1 is a potential biomarker of choroid thickness change in myopia

PURPOSE: To explore the possible molecular mechanism by which epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) regulates choroid thickness (CT) in the development of myopia. METHODS: In total, 131 subjects were divided into the emmetropia (EM) group, non-high m...

Descripción completa

Detalles Bibliográficos
Autores principales: Shi, Wen-Qing, Wan, Ting, Li, Bing, Li, Tao, Zhou, Xiao-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9987712/
https://www.ncbi.nlm.nih.gov/pubmed/36891459
http://dx.doi.org/10.3389/fnins.2023.1144421
_version_ 1784901437287825408
author Shi, Wen-Qing
Wan, Ting
Li, Bing
Li, Tao
Zhou, Xiao-Dong
author_facet Shi, Wen-Qing
Wan, Ting
Li, Bing
Li, Tao
Zhou, Xiao-Dong
author_sort Shi, Wen-Qing
collection PubMed
description PURPOSE: To explore the possible molecular mechanism by which epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) regulates choroid thickness (CT) in the development of myopia. METHODS: In total, 131 subjects were divided into the emmetropia (EM) group, non-high myopia (non-HM) group and high myopia (HM) group. Their age, refraction, intraocular pressure, and other ocular biometric parameters were collected. A 6 × 6 mm area centered on the optic disc was scanned by coherent optical tomography angiography (OCTA) to measure CT, and the tear concentrations of EFEMP1 were quantified using enzyme-linked immunosorbent assay (ELISA) analysis. Twenty-two guinea pigs were divided into the control group and the form-deprivation myopia (FDM) group. The right eye of the guinea pig in the FDM group was covered for 4 weeks, and the diopter and axial length of the right eye of the guinea pig were measured before and after the treatment. After the measurement, the guinea pig was euthanized, and the eyeball was removed. Quantitative reverse transcription polymerase chain reaction, western blotting assays and immunohistochemistry were used to assess the expression of EFEMP1 in the choroid. RESULTS: There were significant differences in CT among the three groups (p < 0.001). CT was positively correlated with age in HM (r = −0.3613, p = 0.0021), but no significant correlation with SE (p > 0.05) was observed. Furthermore, there were increased levels of EFEMP1 in the tears of myopic patients. After 4 weeks of covering the right eye of the FDM guinea pigs, there was a significant increase in axial length and a decrease in diopter (p < 0.05). The mRNA and protein expression of EFEMP1 was significantly increased in the choroid. CONCLUSION: Choroidal thickness was significantly thinner in myopic patients, and the expression level of EFEMP1 in the choroid increased during the development of FDM. Therefore, EFEMP1 may be involved in the regulation of choroidal thickness in myopia patients.
format Online
Article
Text
id pubmed-9987712
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-99877122023-03-07 EFEMP1 is a potential biomarker of choroid thickness change in myopia Shi, Wen-Qing Wan, Ting Li, Bing Li, Tao Zhou, Xiao-Dong Front Neurosci Neuroscience PURPOSE: To explore the possible molecular mechanism by which epidermal growth factor-containing fibulin-like extracellular matrix protein 1 (EFEMP1) regulates choroid thickness (CT) in the development of myopia. METHODS: In total, 131 subjects were divided into the emmetropia (EM) group, non-high myopia (non-HM) group and high myopia (HM) group. Their age, refraction, intraocular pressure, and other ocular biometric parameters were collected. A 6 × 6 mm area centered on the optic disc was scanned by coherent optical tomography angiography (OCTA) to measure CT, and the tear concentrations of EFEMP1 were quantified using enzyme-linked immunosorbent assay (ELISA) analysis. Twenty-two guinea pigs were divided into the control group and the form-deprivation myopia (FDM) group. The right eye of the guinea pig in the FDM group was covered for 4 weeks, and the diopter and axial length of the right eye of the guinea pig were measured before and after the treatment. After the measurement, the guinea pig was euthanized, and the eyeball was removed. Quantitative reverse transcription polymerase chain reaction, western blotting assays and immunohistochemistry were used to assess the expression of EFEMP1 in the choroid. RESULTS: There were significant differences in CT among the three groups (p < 0.001). CT was positively correlated with age in HM (r = −0.3613, p = 0.0021), but no significant correlation with SE (p > 0.05) was observed. Furthermore, there were increased levels of EFEMP1 in the tears of myopic patients. After 4 weeks of covering the right eye of the FDM guinea pigs, there was a significant increase in axial length and a decrease in diopter (p < 0.05). The mRNA and protein expression of EFEMP1 was significantly increased in the choroid. CONCLUSION: Choroidal thickness was significantly thinner in myopic patients, and the expression level of EFEMP1 in the choroid increased during the development of FDM. Therefore, EFEMP1 may be involved in the regulation of choroidal thickness in myopia patients. Frontiers Media S.A. 2023-02-20 /pmc/articles/PMC9987712/ /pubmed/36891459 http://dx.doi.org/10.3389/fnins.2023.1144421 Text en Copyright © 2023 Shi, Wan, Li, Li and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Shi, Wen-Qing
Wan, Ting
Li, Bing
Li, Tao
Zhou, Xiao-Dong
EFEMP1 is a potential biomarker of choroid thickness change in myopia
title EFEMP1 is a potential biomarker of choroid thickness change in myopia
title_full EFEMP1 is a potential biomarker of choroid thickness change in myopia
title_fullStr EFEMP1 is a potential biomarker of choroid thickness change in myopia
title_full_unstemmed EFEMP1 is a potential biomarker of choroid thickness change in myopia
title_short EFEMP1 is a potential biomarker of choroid thickness change in myopia
title_sort efemp1 is a potential biomarker of choroid thickness change in myopia
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9987712/
https://www.ncbi.nlm.nih.gov/pubmed/36891459
http://dx.doi.org/10.3389/fnins.2023.1144421
work_keys_str_mv AT shiwenqing efemp1isapotentialbiomarkerofchoroidthicknesschangeinmyopia
AT wanting efemp1isapotentialbiomarkerofchoroidthicknesschangeinmyopia
AT libing efemp1isapotentialbiomarkerofchoroidthicknesschangeinmyopia
AT litao efemp1isapotentialbiomarkerofchoroidthicknesschangeinmyopia
AT zhouxiaodong efemp1isapotentialbiomarkerofchoroidthicknesschangeinmyopia