A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M

The development and validation of the recombinant 9E1 monoclonal antibody against channel catfish IgM is described. The variable heavy and light chain domains of the 9E1 hybridoma were cloned into murine IgG1 and IgK expression vectors. These expression plasmids were co-transfected into 293F cells a...

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Autores principales: Lange, Miles D., Churchman, Emily M., Wise, Allison L., Bruce, Timothy J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9988478/
https://www.ncbi.nlm.nih.gov/pubmed/36895760
http://dx.doi.org/10.1016/j.fsirep.2023.100086
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author Lange, Miles D.
Churchman, Emily M.
Wise, Allison L.
Bruce, Timothy J.
author_facet Lange, Miles D.
Churchman, Emily M.
Wise, Allison L.
Bruce, Timothy J.
author_sort Lange, Miles D.
collection PubMed
description The development and validation of the recombinant 9E1 monoclonal antibody against channel catfish IgM is described. The variable heavy and light chain domains of the 9E1 hybridoma were cloned into murine IgG1 and IgK expression vectors. These expression plasmids were co-transfected into 293F cells and mature IgG was purified from culture supernatant. It is demonstrated that the recombinant 9E1 monoclonal antibody binds to soluble IgM in ELISA and ELISPOT assays and to membrane-bound IgM by immunofluorescence with different B-cell types. The recombinant 9E1 monoclonal antibody will be a valuable tool in the continued examination of the channel catfish adaptive immune system
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spelling pubmed-99884782023-03-08 A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M Lange, Miles D. Churchman, Emily M. Wise, Allison L. Bruce, Timothy J. Fish Shellfish Immunol Rep Article The development and validation of the recombinant 9E1 monoclonal antibody against channel catfish IgM is described. The variable heavy and light chain domains of the 9E1 hybridoma were cloned into murine IgG1 and IgK expression vectors. These expression plasmids were co-transfected into 293F cells and mature IgG was purified from culture supernatant. It is demonstrated that the recombinant 9E1 monoclonal antibody binds to soluble IgM in ELISA and ELISPOT assays and to membrane-bound IgM by immunofluorescence with different B-cell types. The recombinant 9E1 monoclonal antibody will be a valuable tool in the continued examination of the channel catfish adaptive immune system Elsevier 2023-02-18 /pmc/articles/PMC9988478/ /pubmed/36895760 http://dx.doi.org/10.1016/j.fsirep.2023.100086 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Lange, Miles D.
Churchman, Emily M.
Wise, Allison L.
Bruce, Timothy J.
A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M
title A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M
title_full A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M
title_fullStr A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M
title_full_unstemmed A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M
title_short A recombinant 9E1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin M
title_sort recombinant 9e1 monoclonal antibody binds membrane and soluble channel catfish immunoglobulin m
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9988478/
https://www.ncbi.nlm.nih.gov/pubmed/36895760
http://dx.doi.org/10.1016/j.fsirep.2023.100086
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