Targeted delivery of therapeutic agents to the mouse brain using a stereotactic-guided focused ultrasound device

Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small anima...

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Detalles Bibliográficos
Autores principales: Hu, Zhongtao, Yang, Yaoheng, Ye, Dezhuang, Chen, Si, Gong, Yan, Chukwu, Chinwendu, Chen, Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9988659/
https://www.ncbi.nlm.nih.gov/pubmed/36861835
http://dx.doi.org/10.1016/j.xpro.2023.102132
Descripción
Sumario:Existing protocols of focused ultrasound (FUS) combined with microbubble-mediated blood-brain barrier (BBB) opening (FUS-BBBO) in preclinical research require expensive ultrasound equipment and complex operating procedures. We developed a low-cost, easy-to-use, and precise FUS device for small animal models in preclinical research. Here, we provide a detailed protocol for building the FUS transducer, attaching the transducer to a stereotactic frame for precise brain targeting, applying the integrated FUS device to perform FUS-BBBO in mice, and evaluating the FUS-BBBO outcome. For complete details on the use and execution of this protocol, please refer to Hu et al. (2022).(1)

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