Cargando…

Visualization of α-synuclein trafficking via nanogold labeling and electron microscopy

There is conflicting evidence regarding the mechanisms of α-synuclein internalization, and its trafficking itinerary following cellular entry remains largely unknown. To examine these issues, we describe steps for coupling α-synuclein preformed fibrils (PFFs) to nanogold beads and their subsequent c...

Descripción completa

Detalles Bibliográficos
Autores principales:	Bayati, Armin, Luo, Wen, Del Cid-Pellitero, Esther, Fon, Edward A., Durcan, Thomas M., McPherson, Peter S.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9988663/ https://www.ncbi.nlm.nih.gov/pubmed/36861831 http://dx.doi.org/10.1016/j.xpro.2023.102113

Ejemplares similares

The Dual-Mode Imaging of Nanogold-Labeled Cells by Photoacoustic Microscopy and Fluorescence Optical Microscopy
por: Zhang, Yu, et al.
Publicado: (2018)

Quantitative expansion microscopy for the characterization of the spectrin periodic skeleton of axons using fluorescence microscopy
por: Martínez, Gaby F., et al.
Publicado: (2020)

Development of an α-synuclein knockdown peptide and evaluation of its efficacy in Parkinson’s disease models
por: Jin, Jack Wuyang, et al.
Publicado: (2021)

Ultrastructural Analysis of Nanogold-Labeled Cell Surface Microvilli in Liquid by Atmospheric Scanning Electron Microscopy and Their Relevance in Cell Adhesion
por: Murai, Toshiyuki, et al.
Publicado: (2013)

Hierarchical Nanogold Labels to Improve the Sensitivity of Lateral Flow Immunoassay
por: Serebrennikova, Kseniya, et al.
Publicado: (2017)

A light-inducible protein clustering system for in vivo analysis of α-synuclein aggregation in Parkinson disease
por: Bérard, Morgan, et al.
Publicado: (2022)

Transmission electron microscopy characterization of fluorescently labelled amyloid β 1-40 and α-synuclein aggregates
por: Anderson, Valerie L, et al.
Publicado: (2011)

The Neuroprotective Role of Protein Quality Control in Halting the Development of Alpha-Synuclein Pathology
por: Manecka, Destiny-Love, et al.
Publicado: (2017)

Clathrin-dependent and independent endocytic pathways in tobacco protoplasts revealed by labelling with charged nanogold
por: Onelli, Elisabetta, et al.
Publicado: (2008)

USP19 deubiquitinase inactivation regulates α-synuclein ubiquitination and inhibits accumulation of Lewy body-like aggregates in mice
por: Schorova, Lenka, et al.
Publicado: (2023)

Stress-inducible phosphoprotein 1 (HOP/STI1/STIP1) regulates the accumulation and toxicity of α-synuclein in vivo
por: Lackie, Rachel E., et al.
Publicado: (2022)

Automatic quantification of myocardial remodeling features in human ventricular tissue from label-free microscopy
por: García-Mendívil, Laura, et al.
Publicado: (2023)

Retro-orbital CD45 antibody labeling to evaluate antibody-secreting cell trafficking in mice
por: Pioli, KimAnh T., et al.
Publicado: (2023)

α-Synuclein Trafficking in Parkinson’s Disease: Insights From Fly and Mouse Models
por: Cheng, Jingjing, et al.
Publicado: (2018)

Effects of Excess Brain-Derived Human α-Synuclein on Synaptic Vesicle Trafficking
por: Román-Vendrell, Cristina, et al.
Publicado: (2021)

An oligonucleotide microarray for microRNA expression analysis based on labeling RNA with quantum dot and nanogold probe
por: Liang, Ru-Qiang, et al.
Publicado: (2005)

Protocol for Extraction and Electron Microscopy Visualization of Lipids in Viburnum tinus Fruit Using Cryo-Ultramicrotomy
por: Sinnott-Armstrong, Miranda, et al.
Publicado: (2020)

In Vitro Reconstitution and Imaging of Microtubule Dynamics by Fluorescence and Label-free Microscopy
por: Hirst, William Graham, et al.
Publicado: (2020)

Visualizing Intracellular SNARE Trafficking by Fluorescence Lifetime Imaging Microscopy
por: Verboogen, Daniëlle R. J., et al.
Publicado: (2017)

Lipid species dependent vesicles clustering caused by alpha-synuclein as revealed by single-vesicle imaging with total internal reflection fluorescence microscopy
por: Aryal, Chinta Mani, et al.
Publicado: (2021)

Sublethal Effects of Ionic and Nanogold on the Nematode Caenorhabditis elegans
por: Bosch, Suanne, et al.
Publicado: (2018)

Green nanogold activity in experimental breast carcinoma in vivo
por: Hendi, Awatif A., et al.
Publicado: (2020)

Hsc70 rescues the synaptic vesicle trafficking defects caused by α-synuclein dimers
por: Brady, Emily B., et al.
Publicado: (2023)

Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy
por: Ghoneim, Mohamed, et al.
Publicado: (2023)

Contrast-Enhanced Tissue Processing of Fibrillin-Rich Elastic Fibres for 3D Visualization by Volume Scanning Electron Microscopy
por: Lewis, Philip N., et al.
Publicado: (2021)

Visualizing leukocyte trafficking in the living brain with 2-photon intravital microscopy
por: Pai, Saparna, et al.
Publicado: (2013)

Preparation of Nanosilver and Nanogold Based on Dog Rose Aqueous Extract
por: Pulit, Jolanta, et al.
Publicado: (2014)

Ultrasmall dopamine-coated nanogolds: preparation, characteristics, and CT imaging
por: Yu, Yao, et al.
Publicado: (2016)

Evaluation of nanoselenium and nanogold activities against murine intestinal schistosomiasis
por: Dkhil, Mohamed A., et al.
Publicado: (2019)

Effective and reproducible biosynthesis of nanogold-composite catalyst for paracetamol oxidation
por: Krbečková, Veronika, et al.
Publicado: (2022)

Bacterial DNA Recognition by SERS Active Plasma-Coupled Nanogold
por: Shvalya, Vasyl, et al.
Publicado: (2022)

SARS-CoV-2 infects cells after viral entry via clathrin-mediated endocytosis
por: Bayati, Armin, et al.
Publicado: (2021)

Expansion Microscopy for Beginners: Visualizing Microtubules in Expanded Cultured HeLa Cells
por: Zhang, Chi, et al.
Publicado: (2020)

Visualizing murine breast and melanoma tumor microenvironment using intravital multiphoton microscopy
por: Jain, Rohit, et al.
Publicado: (2021)

A protocol to visualize cytosolic aggresome-like bodies using confocal microscopy
por: Kim, Soyeon, et al.
Publicado: (2021)

DIRECT VISUALIZATION OF LABELED NUCLEOTIDES IN DNA USING ELECTRON MICROSCOPY AND AUTORADIOGRAPHY
por: Weber, G. H., et al.
Publicado: (1974)

Three-dimensional structural dynamics and fluctuations of DNA-nanogold conjugates by individual-particle electron tomography
por: Zhang, Lei, et al.
Publicado: (2016)

Protocol to visualize glycan-mediated binding between virus and human blood mononuclear cells using thin-section transmission electron microscopy
por: Spillings, Belinda L., et al.
Publicado: (2023)

Real-time visualization of exo- and endocytosis membrane dynamics with confocal and super-resolution microscopy
por: Guo, Xiaoli, et al.
Publicado: (2022)

Monitoring α-synuclein ubiquitination dynamics reveals key endosomal effectors mediating its trafficking and degradation
por: Zenko, Dmitry, et al.
Publicado: (2023)

Cannot write session to /tmp/vufind_sessions/sess_3hko3d1okoavqrfhid03kp1nqd