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Protocol to culture and image pancreatic ductal adenocarcinoma tumor slices to study T cell migration
Here, we describe a protocol for culture and live cell imaging of tumor slices. This approach studies carcinoma and immune cell dynamics in complex tumor microenvironments (TME) with nonlinear optical imaging platforms. Using a tumor-bearing mouse model of pancreatic ductal adenocarcinoma (PDA), we...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9988667/ https://www.ncbi.nlm.nih.gov/pubmed/36861840 http://dx.doi.org/10.1016/j.xpro.2023.102135 |