Protocol to culture and image pancreatic ductal adenocarcinoma tumor slices to study T cell migration

Here, we describe a protocol for culture and live cell imaging of tumor slices. This approach studies carcinoma and immune cell dynamics in complex tumor microenvironments (TME) with nonlinear optical imaging platforms. Using a tumor-bearing mouse model of pancreatic ductal adenocarcinoma (PDA), we...

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Detalles Bibliográficos
Autores principales: Rodríguez-Merced, Nelson J., Callaway, Mackenzie K., Provenzano, Paolo P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9988667/
https://www.ncbi.nlm.nih.gov/pubmed/36861840
http://dx.doi.org/10.1016/j.xpro.2023.102135

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