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Comparative analysis of drought stress-induced physiological and transcriptional changes of two black sesame cultivars during anthesis

Sesame production is severely affected by unexpected drought stress during flowering stage. However, little is known about dynamic drought-responsive mechanisms during anthesis in sesame, and no particular attention was given to black sesame, the most common ingredient in East Asia traditional medic...

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Autores principales: Wang, Xiaohui, Wang, Min, Yan, Gui, Yang, Huiyi, Wei, Guangwei, Shen, Tinghai, Wan, Zehua, Zheng, Wei, Fang, Sheng, Wu, Ziming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9989188/
https://www.ncbi.nlm.nih.gov/pubmed/36895884
http://dx.doi.org/10.3389/fpls.2023.1117507
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author Wang, Xiaohui
Wang, Min
Yan, Gui
Yang, Huiyi
Wei, Guangwei
Shen, Tinghai
Wan, Zehua
Zheng, Wei
Fang, Sheng
Wu, Ziming
author_facet Wang, Xiaohui
Wang, Min
Yan, Gui
Yang, Huiyi
Wei, Guangwei
Shen, Tinghai
Wan, Zehua
Zheng, Wei
Fang, Sheng
Wu, Ziming
author_sort Wang, Xiaohui
collection PubMed
description Sesame production is severely affected by unexpected drought stress during flowering stage. However, little is known about dynamic drought-responsive mechanisms during anthesis in sesame, and no particular attention was given to black sesame, the most common ingredient in East Asia traditional medicine. Herein, we investigated drought-responsive mechanisms of two contrasting black sesame cultivars (Jinhuangma, JHM, and Poyanghei, PYH) during anthesis. Compared to PYH, JHM plants showed higher tolerance to drought stress through the maintenance of biological membrane properties, high induction of osmoprotectants’ biosynthesis and accumulation, and significant enhancement of the activities of antioxidant enzymes. For instance, the drought stress induced a significant increase in the content of soluble protein (SP), soluble sugar (SS), proline (PRO), glutathione (GSH), as well as the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in leaves and roots of JHM plants compared to PYH plants. RNA sequencing followed by differentially expressed genes (DEGs) analysis revealed that more genes were significantly induced under drought in JHM than in PYH plants. Functional enrichment analyses disclosed that several pathways related to drought stress tolerance, such as photosynthesis, amino acids and fatty acid metabolisms, peroxisome, ascorbate and aldarate metabolism, plant hormone signal transduction, biosynthesis of secondary metabolites, and glutathione metabolism, were highly stimulated in JHM than in PYH plants. Thirty-one (31) key highly induced DEGs, including transcription factors and glutathione reductase and ethylene biosynthetic genes, were identified as potential candidate genes for improving black sesame drought stress tolerance. Our findings show that a strong antioxidant system, biosynthesis and accumulation of osmoprotectants, TFs (mainly ERFs and NACs), and phytohormones are essential for black sesame drought tolerance. Moreover, they provide resources for functional genomic studies toward molecular breeding of drought-tolerant black sesame varieties.
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spelling pubmed-99891882023-03-08 Comparative analysis of drought stress-induced physiological and transcriptional changes of two black sesame cultivars during anthesis Wang, Xiaohui Wang, Min Yan, Gui Yang, Huiyi Wei, Guangwei Shen, Tinghai Wan, Zehua Zheng, Wei Fang, Sheng Wu, Ziming Front Plant Sci Plant Science Sesame production is severely affected by unexpected drought stress during flowering stage. However, little is known about dynamic drought-responsive mechanisms during anthesis in sesame, and no particular attention was given to black sesame, the most common ingredient in East Asia traditional medicine. Herein, we investigated drought-responsive mechanisms of two contrasting black sesame cultivars (Jinhuangma, JHM, and Poyanghei, PYH) during anthesis. Compared to PYH, JHM plants showed higher tolerance to drought stress through the maintenance of biological membrane properties, high induction of osmoprotectants’ biosynthesis and accumulation, and significant enhancement of the activities of antioxidant enzymes. For instance, the drought stress induced a significant increase in the content of soluble protein (SP), soluble sugar (SS), proline (PRO), glutathione (GSH), as well as the activities of superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) in leaves and roots of JHM plants compared to PYH plants. RNA sequencing followed by differentially expressed genes (DEGs) analysis revealed that more genes were significantly induced under drought in JHM than in PYH plants. Functional enrichment analyses disclosed that several pathways related to drought stress tolerance, such as photosynthesis, amino acids and fatty acid metabolisms, peroxisome, ascorbate and aldarate metabolism, plant hormone signal transduction, biosynthesis of secondary metabolites, and glutathione metabolism, were highly stimulated in JHM than in PYH plants. Thirty-one (31) key highly induced DEGs, including transcription factors and glutathione reductase and ethylene biosynthetic genes, were identified as potential candidate genes for improving black sesame drought stress tolerance. Our findings show that a strong antioxidant system, biosynthesis and accumulation of osmoprotectants, TFs (mainly ERFs and NACs), and phytohormones are essential for black sesame drought tolerance. Moreover, they provide resources for functional genomic studies toward molecular breeding of drought-tolerant black sesame varieties. Frontiers Media S.A. 2023-02-21 /pmc/articles/PMC9989188/ /pubmed/36895884 http://dx.doi.org/10.3389/fpls.2023.1117507 Text en Copyright © 2023 Wang, Wang, Yan, Yang, Wei, Shen, Wan, Zheng, Fang and Wu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Wang, Xiaohui
Wang, Min
Yan, Gui
Yang, Huiyi
Wei, Guangwei
Shen, Tinghai
Wan, Zehua
Zheng, Wei
Fang, Sheng
Wu, Ziming
Comparative analysis of drought stress-induced physiological and transcriptional changes of two black sesame cultivars during anthesis
title Comparative analysis of drought stress-induced physiological and transcriptional changes of two black sesame cultivars during anthesis
title_full Comparative analysis of drought stress-induced physiological and transcriptional changes of two black sesame cultivars during anthesis
title_fullStr Comparative analysis of drought stress-induced physiological and transcriptional changes of two black sesame cultivars during anthesis
title_full_unstemmed Comparative analysis of drought stress-induced physiological and transcriptional changes of two black sesame cultivars during anthesis
title_short Comparative analysis of drought stress-induced physiological and transcriptional changes of two black sesame cultivars during anthesis
title_sort comparative analysis of drought stress-induced physiological and transcriptional changes of two black sesame cultivars during anthesis
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9989188/
https://www.ncbi.nlm.nih.gov/pubmed/36895884
http://dx.doi.org/10.3389/fpls.2023.1117507
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