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Novel virulence factor Cba induces antibody-dependent enhancement (ADE) of Streptococcus suis Serotype 9 infection in a mouse model

Streptococcus suis (SS) is a zoonotic pathogen that affects the health of humans and the development of the pig industry. The SS Cba protein is a collagen adhesin, and a few of its homologs are related to the enhancement of bacterial adhesion. We compared the phenotypes of SS9-P10, SS9-P10 cba knock...

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Autores principales: Yang, Pengjiang, Yang, Lei, Cao, Kun, Hu, Qian, Hu, Yuli, Shi, Jun, Zhao, Dun, Yu, Xinglong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9989217/
https://www.ncbi.nlm.nih.gov/pubmed/36896190
http://dx.doi.org/10.3389/fcimb.2023.1027419
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author Yang, Pengjiang
Yang, Lei
Cao, Kun
Hu, Qian
Hu, Yuli
Shi, Jun
Zhao, Dun
Yu, Xinglong
author_facet Yang, Pengjiang
Yang, Lei
Cao, Kun
Hu, Qian
Hu, Yuli
Shi, Jun
Zhao, Dun
Yu, Xinglong
author_sort Yang, Pengjiang
collection PubMed
description Streptococcus suis (SS) is a zoonotic pathogen that affects the health of humans and the development of the pig industry. The SS Cba protein is a collagen adhesin, and a few of its homologs are related to the enhancement of bacterial adhesion. We compared the phenotypes of SS9-P10, SS9-P10 cba knockout strains and its complementary strains in vitro and in vivo and found that knocking out the cba gene did not affect the growth characteristics of the strain, but it significantly reduced the ability of SS to form biofilms, adhesion to host cells, phagocytic resistance to macrophages and attenuated virulence in a mouse infection model. These results indicated that Cba was a virulence related factor of SS9. In addition, Mice immunized with the Cba protein had higher mortality and more serious organ lesions after challenge, and the same was observed in passive immunization experiments. This phenomenon is similar to the antibody-dependent enhancement of infection by bacteria such as Acinetobacter baumannii and Streptococcus pneumoniae. To our knowledge, this is the first demonstration of antibody-dependent enhancement of SS, and these observations highlight the complexity of antibody-based therapy for SS infection.
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spelling pubmed-99892172023-03-08 Novel virulence factor Cba induces antibody-dependent enhancement (ADE) of Streptococcus suis Serotype 9 infection in a mouse model Yang, Pengjiang Yang, Lei Cao, Kun Hu, Qian Hu, Yuli Shi, Jun Zhao, Dun Yu, Xinglong Front Cell Infect Microbiol Cellular and Infection Microbiology Streptococcus suis (SS) is a zoonotic pathogen that affects the health of humans and the development of the pig industry. The SS Cba protein is a collagen adhesin, and a few of its homologs are related to the enhancement of bacterial adhesion. We compared the phenotypes of SS9-P10, SS9-P10 cba knockout strains and its complementary strains in vitro and in vivo and found that knocking out the cba gene did not affect the growth characteristics of the strain, but it significantly reduced the ability of SS to form biofilms, adhesion to host cells, phagocytic resistance to macrophages and attenuated virulence in a mouse infection model. These results indicated that Cba was a virulence related factor of SS9. In addition, Mice immunized with the Cba protein had higher mortality and more serious organ lesions after challenge, and the same was observed in passive immunization experiments. This phenomenon is similar to the antibody-dependent enhancement of infection by bacteria such as Acinetobacter baumannii and Streptococcus pneumoniae. To our knowledge, this is the first demonstration of antibody-dependent enhancement of SS, and these observations highlight the complexity of antibody-based therapy for SS infection. Frontiers Media S.A. 2023-02-21 /pmc/articles/PMC9989217/ /pubmed/36896190 http://dx.doi.org/10.3389/fcimb.2023.1027419 Text en Copyright © 2023 Yang, Yang, Cao, Hu, Hu, Shi, Zhao and Yu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Yang, Pengjiang
Yang, Lei
Cao, Kun
Hu, Qian
Hu, Yuli
Shi, Jun
Zhao, Dun
Yu, Xinglong
Novel virulence factor Cba induces antibody-dependent enhancement (ADE) of Streptococcus suis Serotype 9 infection in a mouse model
title Novel virulence factor Cba induces antibody-dependent enhancement (ADE) of Streptococcus suis Serotype 9 infection in a mouse model
title_full Novel virulence factor Cba induces antibody-dependent enhancement (ADE) of Streptococcus suis Serotype 9 infection in a mouse model
title_fullStr Novel virulence factor Cba induces antibody-dependent enhancement (ADE) of Streptococcus suis Serotype 9 infection in a mouse model
title_full_unstemmed Novel virulence factor Cba induces antibody-dependent enhancement (ADE) of Streptococcus suis Serotype 9 infection in a mouse model
title_short Novel virulence factor Cba induces antibody-dependent enhancement (ADE) of Streptococcus suis Serotype 9 infection in a mouse model
title_sort novel virulence factor cba induces antibody-dependent enhancement (ade) of streptococcus suis serotype 9 infection in a mouse model
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9989217/
https://www.ncbi.nlm.nih.gov/pubmed/36896190
http://dx.doi.org/10.3389/fcimb.2023.1027419
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