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Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance

DNA-binding with one finger (Dof) transcription factors play a crucial role in plant abiotic stress regulatory networks, although massive Dofs have been systematically characterized in plants, they have not been identified in the hexaploid crop sweetpotato. Herein, 43 IbDof genes were detected to be...

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Autores principales: Zhang, Chengbin, Dong, Tingting, Yu, Jing, Hong, Haiting, Liu, Siyuan, Guo, Fen, Ma, Hongting, Zhang, Jianling, Zhu, Mingku, Meng, Xiaoqing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9989284/
https://www.ncbi.nlm.nih.gov/pubmed/36895872
http://dx.doi.org/10.3389/fpls.2023.1140727
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author Zhang, Chengbin
Dong, Tingting
Yu, Jing
Hong, Haiting
Liu, Siyuan
Guo, Fen
Ma, Hongting
Zhang, Jianling
Zhu, Mingku
Meng, Xiaoqing
author_facet Zhang, Chengbin
Dong, Tingting
Yu, Jing
Hong, Haiting
Liu, Siyuan
Guo, Fen
Ma, Hongting
Zhang, Jianling
Zhu, Mingku
Meng, Xiaoqing
author_sort Zhang, Chengbin
collection PubMed
description DNA-binding with one finger (Dof) transcription factors play a crucial role in plant abiotic stress regulatory networks, although massive Dofs have been systematically characterized in plants, they have not been identified in the hexaploid crop sweetpotato. Herein, 43 IbDof genes were detected to be disproportionally dispersed across 14 of the 15 chromosomes of sweetpotato, and segmental duplications were discovered to be the major driving force for the expansion of IbDofs. The collinearity analysis of IbDofs with their related orthologs from eight plants revealed the potential evolutionary history of Dof gene family. Phylogenetic analysis displayed that IbDof proteins were assigned into nine subfamilies, and the regularity of gene structures and conserved motifs was consistent with the subgroup classification. Additionally, five chosen IbDof genes were shown to be substantially and variably induced under various abiotic conditions (salt, drought, heat, and cold), as well as hormone treatments (ABA and SA), according to their transcriptome data and qRT-PCR experiments. Consistently, the promoters of IbDofs contained a number of cis-acting elements associated with hormone and stress responses. Besides, it was noted that IbDof2 had transactivation activity in yeasts, while IbDof-11/-16/-36 did not, and protein interaction network analysis and yeast two-hybrid experiments revealed a complicated interaction connection amongst IbDofs. Collectively, these data lay a foundation for further functional explorations of IbDof genes, especially with regards to the possible application of multiple IbDof members in breeding the tolerant plants.
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spelling pubmed-99892842023-03-08 Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance Zhang, Chengbin Dong, Tingting Yu, Jing Hong, Haiting Liu, Siyuan Guo, Fen Ma, Hongting Zhang, Jianling Zhu, Mingku Meng, Xiaoqing Front Plant Sci Plant Science DNA-binding with one finger (Dof) transcription factors play a crucial role in plant abiotic stress regulatory networks, although massive Dofs have been systematically characterized in plants, they have not been identified in the hexaploid crop sweetpotato. Herein, 43 IbDof genes were detected to be disproportionally dispersed across 14 of the 15 chromosomes of sweetpotato, and segmental duplications were discovered to be the major driving force for the expansion of IbDofs. The collinearity analysis of IbDofs with their related orthologs from eight plants revealed the potential evolutionary history of Dof gene family. Phylogenetic analysis displayed that IbDof proteins were assigned into nine subfamilies, and the regularity of gene structures and conserved motifs was consistent with the subgroup classification. Additionally, five chosen IbDof genes were shown to be substantially and variably induced under various abiotic conditions (salt, drought, heat, and cold), as well as hormone treatments (ABA and SA), according to their transcriptome data and qRT-PCR experiments. Consistently, the promoters of IbDofs contained a number of cis-acting elements associated with hormone and stress responses. Besides, it was noted that IbDof2 had transactivation activity in yeasts, while IbDof-11/-16/-36 did not, and protein interaction network analysis and yeast two-hybrid experiments revealed a complicated interaction connection amongst IbDofs. Collectively, these data lay a foundation for further functional explorations of IbDof genes, especially with regards to the possible application of multiple IbDof members in breeding the tolerant plants. Frontiers Media S.A. 2023-02-21 /pmc/articles/PMC9989284/ /pubmed/36895872 http://dx.doi.org/10.3389/fpls.2023.1140727 Text en Copyright © 2023 Zhang, Dong, Yu, Hong, Liu, Guo, Ma, Zhang, Zhu and Meng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Zhang, Chengbin
Dong, Tingting
Yu, Jing
Hong, Haiting
Liu, Siyuan
Guo, Fen
Ma, Hongting
Zhang, Jianling
Zhu, Mingku
Meng, Xiaoqing
Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance
title Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance
title_full Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance
title_fullStr Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance
title_full_unstemmed Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance
title_short Genome-wide survey and expression analysis of Dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance
title_sort genome-wide survey and expression analysis of dof transcription factor family in sweetpotato shed light on their promising functions in stress tolerance
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9989284/
https://www.ncbi.nlm.nih.gov/pubmed/36895872
http://dx.doi.org/10.3389/fpls.2023.1140727
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