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Kerosene Biodegradation by Highly Efficient Indigenous Bacteria Isolated From Hydrocarbon-Contaminated Sites

Kerosene is widely used in Ethiopia as a household fuel (for lighting and heating), as a solvent in paint and grease, and as a lubricant in glass cutting. It causes environmental pollution and escorts to loss of ecological functioning and health problems. Therefore, this research was designed to iso...

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Autores principales: Bekele, Gessesse Kebede, Gebrie, Solomon Abera, Abda, Ebrahim M., Sinshaw, Gebiru, Haregu, Simatsidk, Negie, Zemene Worku, Tafesse, Mesfin, Assefa, Fasil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9989413/
https://www.ncbi.nlm.nih.gov/pubmed/36895787
http://dx.doi.org/10.1177/11786361221150759
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author Bekele, Gessesse Kebede
Gebrie, Solomon Abera
Abda, Ebrahim M.
Sinshaw, Gebiru
Haregu, Simatsidk
Negie, Zemene Worku
Tafesse, Mesfin
Assefa, Fasil
author_facet Bekele, Gessesse Kebede
Gebrie, Solomon Abera
Abda, Ebrahim M.
Sinshaw, Gebiru
Haregu, Simatsidk
Negie, Zemene Worku
Tafesse, Mesfin
Assefa, Fasil
author_sort Bekele, Gessesse Kebede
collection PubMed
description Kerosene is widely used in Ethiopia as a household fuel (for lighting and heating), as a solvent in paint and grease, and as a lubricant in glass cutting. It causes environmental pollution and escorts to loss of ecological functioning and health problems. Therefore, this research was designed to isolate, identify, and characterize indigenous kerosene-degrading bacteria that are effective in cleaning ecological units that have been contaminated by kerosene. Soil samples were collected from hydrocarbon-contaminated sites (flower farms, garages, and old-aged asphalt roads) and spread-plated on mineral salt medium (Bushnell Hass Mineral Salts Agar Medium: BHMS), which consists of kerosene as the only carbon source. Seven kerosene-degrading bacterial species were isolated, 2 from flower farms, 3 from garage areas, and 2 from asphalt areas. Three genera from hydrocarbon-contaminated sites were identified, including Pseudomonas, Bacillus, and Acinetobacter using biochemical characterization and the Biolog database. Growth studies in the presence of various concentrations of kerosene (1% and 3% v/v) showed that the bacterial isolates could metabolize kerosene as energy and biomass. Thereby, a gravimetric study was performed on bacterial strains that proliferated well on a BHMS medium with kerosene. Remarkably, bacterial isolates were able to degrade 5% kerosene from 57.2% to 91% in 15 days. Moreover, 2 of the most potent isolates, AUG2 and AUG1, resulted in 85% and 91% kerosene degradation, respectively, when allowed to grow on a medium containing kerosene. In addition, 16S rRNA gene analysis indicated that strain AAUG1 belonged to Bacillus tequilensis, whereas isolate AAUG showed the highest similarity to Bacillus subtilis. Therefore, these indigenous bacterial isolates have the potential to be applied for kerosene removal from hydrocarbon-contaminated sites and the development of remediation approaches.
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spelling pubmed-99894132023-03-08 Kerosene Biodegradation by Highly Efficient Indigenous Bacteria Isolated From Hydrocarbon-Contaminated Sites Bekele, Gessesse Kebede Gebrie, Solomon Abera Abda, Ebrahim M. Sinshaw, Gebiru Haregu, Simatsidk Negie, Zemene Worku Tafesse, Mesfin Assefa, Fasil Microbiol Insights Original Research Kerosene is widely used in Ethiopia as a household fuel (for lighting and heating), as a solvent in paint and grease, and as a lubricant in glass cutting. It causes environmental pollution and escorts to loss of ecological functioning and health problems. Therefore, this research was designed to isolate, identify, and characterize indigenous kerosene-degrading bacteria that are effective in cleaning ecological units that have been contaminated by kerosene. Soil samples were collected from hydrocarbon-contaminated sites (flower farms, garages, and old-aged asphalt roads) and spread-plated on mineral salt medium (Bushnell Hass Mineral Salts Agar Medium: BHMS), which consists of kerosene as the only carbon source. Seven kerosene-degrading bacterial species were isolated, 2 from flower farms, 3 from garage areas, and 2 from asphalt areas. Three genera from hydrocarbon-contaminated sites were identified, including Pseudomonas, Bacillus, and Acinetobacter using biochemical characterization and the Biolog database. Growth studies in the presence of various concentrations of kerosene (1% and 3% v/v) showed that the bacterial isolates could metabolize kerosene as energy and biomass. Thereby, a gravimetric study was performed on bacterial strains that proliferated well on a BHMS medium with kerosene. Remarkably, bacterial isolates were able to degrade 5% kerosene from 57.2% to 91% in 15 days. Moreover, 2 of the most potent isolates, AUG2 and AUG1, resulted in 85% and 91% kerosene degradation, respectively, when allowed to grow on a medium containing kerosene. In addition, 16S rRNA gene analysis indicated that strain AAUG1 belonged to Bacillus tequilensis, whereas isolate AAUG showed the highest similarity to Bacillus subtilis. Therefore, these indigenous bacterial isolates have the potential to be applied for kerosene removal from hydrocarbon-contaminated sites and the development of remediation approaches. SAGE Publications 2023-03-06 /pmc/articles/PMC9989413/ /pubmed/36895787 http://dx.doi.org/10.1177/11786361221150759 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page(https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Research
Bekele, Gessesse Kebede
Gebrie, Solomon Abera
Abda, Ebrahim M.
Sinshaw, Gebiru
Haregu, Simatsidk
Negie, Zemene Worku
Tafesse, Mesfin
Assefa, Fasil
Kerosene Biodegradation by Highly Efficient Indigenous Bacteria Isolated From Hydrocarbon-Contaminated Sites
title Kerosene Biodegradation by Highly Efficient Indigenous Bacteria Isolated From Hydrocarbon-Contaminated Sites
title_full Kerosene Biodegradation by Highly Efficient Indigenous Bacteria Isolated From Hydrocarbon-Contaminated Sites
title_fullStr Kerosene Biodegradation by Highly Efficient Indigenous Bacteria Isolated From Hydrocarbon-Contaminated Sites
title_full_unstemmed Kerosene Biodegradation by Highly Efficient Indigenous Bacteria Isolated From Hydrocarbon-Contaminated Sites
title_short Kerosene Biodegradation by Highly Efficient Indigenous Bacteria Isolated From Hydrocarbon-Contaminated Sites
title_sort kerosene biodegradation by highly efficient indigenous bacteria isolated from hydrocarbon-contaminated sites
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9989413/
https://www.ncbi.nlm.nih.gov/pubmed/36895787
http://dx.doi.org/10.1177/11786361221150759
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