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Characterization of the preferred cation cofactors of chloroplast protein kinases in Arabidopsis thaliana
Chloroplasts sense a variety of stimuli triggering several acclimation responses. One prominent response is the mechanism of state transitions, which enables rapid adaption to changes in illumination. Here, we investigated the link between divalent cations (calcium, magnesium, and manganese) and pro...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9989932/ https://www.ncbi.nlm.nih.gov/pubmed/36683405 http://dx.doi.org/10.1002/2211-5463.13563 |
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author | Espinoza‐Corral, Roberto Schwenkert, Serena Schneider, Anja |
author_facet | Espinoza‐Corral, Roberto Schwenkert, Serena Schneider, Anja |
author_sort | Espinoza‐Corral, Roberto |
collection | PubMed |
description | Chloroplasts sense a variety of stimuli triggering several acclimation responses. One prominent response is the mechanism of state transitions, which enables rapid adaption to changes in illumination. Here, we investigated the link between divalent cations (calcium, magnesium, and manganese) and protein kinase activity in Arabidopsis chloroplasts. Our results show that manganese ions are the strongest activator of kinase activity in chloroplasts followed by magnesium ions, whereas calcium ions are not able to induce kinase activity. Additionally, the phosphorylation of specific protein bands is strongly reduced in chloroplasts of a cmt1 mutant, which is impaired in manganese import into chloroplasts, as compared to the wild‐type. These findings provide insights for the future characterization of chloroplast protein kinase activity and potential target proteins. |
format | Online Article Text |
id | pubmed-9989932 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-99899322023-03-08 Characterization of the preferred cation cofactors of chloroplast protein kinases in Arabidopsis thaliana Espinoza‐Corral, Roberto Schwenkert, Serena Schneider, Anja FEBS Open Bio Research Articles Chloroplasts sense a variety of stimuli triggering several acclimation responses. One prominent response is the mechanism of state transitions, which enables rapid adaption to changes in illumination. Here, we investigated the link between divalent cations (calcium, magnesium, and manganese) and protein kinase activity in Arabidopsis chloroplasts. Our results show that manganese ions are the strongest activator of kinase activity in chloroplasts followed by magnesium ions, whereas calcium ions are not able to induce kinase activity. Additionally, the phosphorylation of specific protein bands is strongly reduced in chloroplasts of a cmt1 mutant, which is impaired in manganese import into chloroplasts, as compared to the wild‐type. These findings provide insights for the future characterization of chloroplast protein kinase activity and potential target proteins. John Wiley and Sons Inc. 2023-01-31 /pmc/articles/PMC9989932/ /pubmed/36683405 http://dx.doi.org/10.1002/2211-5463.13563 Text en © 2023 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Espinoza‐Corral, Roberto Schwenkert, Serena Schneider, Anja Characterization of the preferred cation cofactors of chloroplast protein kinases in Arabidopsis thaliana |
title | Characterization of the preferred cation cofactors of chloroplast protein kinases in Arabidopsis thaliana
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title_full | Characterization of the preferred cation cofactors of chloroplast protein kinases in Arabidopsis thaliana
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title_fullStr | Characterization of the preferred cation cofactors of chloroplast protein kinases in Arabidopsis thaliana
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title_full_unstemmed | Characterization of the preferred cation cofactors of chloroplast protein kinases in Arabidopsis thaliana
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title_short | Characterization of the preferred cation cofactors of chloroplast protein kinases in Arabidopsis thaliana
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title_sort | characterization of the preferred cation cofactors of chloroplast protein kinases in arabidopsis thaliana |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9989932/ https://www.ncbi.nlm.nih.gov/pubmed/36683405 http://dx.doi.org/10.1002/2211-5463.13563 |
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