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Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study

BACKGROUND: This rat study aimed to evaluate the feasibility of temperature thresholds that affect peri-implant bone cells and morphology and the potential usefulness of thermal necrosis for inducing implant removal for a subsequent in vivo pig study. METHODS: On one side, rat tibiae were thermally...

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Autores principales: Kniha, Kristian, Buhl, Eva Miriam, Al-Sibai, Faruk, Möhlhenrich, Stephan Christian, Bock, Anna, Heitzer, Marius, Hölzle, Frank, Modabber, Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9990269/
https://www.ncbi.nlm.nih.gov/pubmed/36882765
http://dx.doi.org/10.1186/s13005-023-00349-2
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author Kniha, Kristian
Buhl, Eva Miriam
Al-Sibai, Faruk
Möhlhenrich, Stephan Christian
Bock, Anna
Heitzer, Marius
Hölzle, Frank
Modabber, Ali
author_facet Kniha, Kristian
Buhl, Eva Miriam
Al-Sibai, Faruk
Möhlhenrich, Stephan Christian
Bock, Anna
Heitzer, Marius
Hölzle, Frank
Modabber, Ali
author_sort Kniha, Kristian
collection PubMed
description BACKGROUND: This rat study aimed to evaluate the feasibility of temperature thresholds that affect peri-implant bone cells and morphology and the potential usefulness of thermal necrosis for inducing implant removal for a subsequent in vivo pig study. METHODS: On one side, rat tibiae were thermally treated before implant insertion. The contralateral side was used as the control group without tempering. Temperatures of 4 °C, 3 °C, 2 °C, 48 °C, 49 °C, and 50 °C were evaluated with a tempering time of 1 min. Energy-dispersive X-ray spectroscopy (EDX) and transmission electron microscopy (TEM) analyses were performed. RESULTS: The EDX analysis revealed significant increases in element weights at 50 °C (e.g., calcium, phosphate, sodium, and sulfur; p < 0.01). The results of the TEM analysis showed that at all the applied cold and warm temperatures, signs of cell damage were observed, including vacuolization, shrinkage, and detachment from the surrounding bone matrix. Some cells became necrotic, leaving the lacunae empty. CONCLUSIONS: Temperature of 50 °C led to irreversible cell death. The degree of damage was more significant at 50 °C and 2 °C than at 48 °C and 5 °C. Although this was a preliminary study, from the results, we identified that a temperature of 50 °C at a time interval of 60 min can lower the number of samples in a further study of thermo-explantation. Thus, the subsequent planned in vivo study in pigs, which will consider osseointegrated implants, is feasible. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13005-023-00349-2.
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spelling pubmed-99902692023-03-08 Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study Kniha, Kristian Buhl, Eva Miriam Al-Sibai, Faruk Möhlhenrich, Stephan Christian Bock, Anna Heitzer, Marius Hölzle, Frank Modabber, Ali Head Face Med Research BACKGROUND: This rat study aimed to evaluate the feasibility of temperature thresholds that affect peri-implant bone cells and morphology and the potential usefulness of thermal necrosis for inducing implant removal for a subsequent in vivo pig study. METHODS: On one side, rat tibiae were thermally treated before implant insertion. The contralateral side was used as the control group without tempering. Temperatures of 4 °C, 3 °C, 2 °C, 48 °C, 49 °C, and 50 °C were evaluated with a tempering time of 1 min. Energy-dispersive X-ray spectroscopy (EDX) and transmission electron microscopy (TEM) analyses were performed. RESULTS: The EDX analysis revealed significant increases in element weights at 50 °C (e.g., calcium, phosphate, sodium, and sulfur; p < 0.01). The results of the TEM analysis showed that at all the applied cold and warm temperatures, signs of cell damage were observed, including vacuolization, shrinkage, and detachment from the surrounding bone matrix. Some cells became necrotic, leaving the lacunae empty. CONCLUSIONS: Temperature of 50 °C led to irreversible cell death. The degree of damage was more significant at 50 °C and 2 °C than at 48 °C and 5 °C. Although this was a preliminary study, from the results, we identified that a temperature of 50 °C at a time interval of 60 min can lower the number of samples in a further study of thermo-explantation. Thus, the subsequent planned in vivo study in pigs, which will consider osseointegrated implants, is feasible. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13005-023-00349-2. BioMed Central 2023-03-07 /pmc/articles/PMC9990269/ /pubmed/36882765 http://dx.doi.org/10.1186/s13005-023-00349-2 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Kniha, Kristian
Buhl, Eva Miriam
Al-Sibai, Faruk
Möhlhenrich, Stephan Christian
Bock, Anna
Heitzer, Marius
Hölzle, Frank
Modabber, Ali
Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study
title Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study
title_full Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study
title_fullStr Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study
title_full_unstemmed Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study
title_short Results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study
title_sort results of thermal osteonecrosis for implant removal on electron microscopy, implant stability, and radiographic parameters – a rat study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9990269/
https://www.ncbi.nlm.nih.gov/pubmed/36882765
http://dx.doi.org/10.1186/s13005-023-00349-2
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