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Tree peony transcription factor PrWRI1 enhances seed oil accumulation
BACKGROUND: WRINKLED1 (WRI1) encodes a transcription factor, belonging to the APETALA2 (AP2) family, and plays a key role in regulating plant oil biosynthesis. As a newly woody oil crop, tree peony (Paeonia rockii) was notable for the abundant unsaturated fatty acids in its seed oil. However, the ro...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9990299/ https://www.ncbi.nlm.nih.gov/pubmed/36882682 http://dx.doi.org/10.1186/s12870-023-04127-9 |
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author | Xie, Lihang Hu, Jiayuan Yan, Zhenguo Li, Xinyao Wei, Sailong Xu, Ruilin Yang, Weizong Gu, Huihui Zhang, Qingyu |
author_facet | Xie, Lihang Hu, Jiayuan Yan, Zhenguo Li, Xinyao Wei, Sailong Xu, Ruilin Yang, Weizong Gu, Huihui Zhang, Qingyu |
author_sort | Xie, Lihang |
collection | PubMed |
description | BACKGROUND: WRINKLED1 (WRI1) encodes a transcription factor, belonging to the APETALA2 (AP2) family, and plays a key role in regulating plant oil biosynthesis. As a newly woody oil crop, tree peony (Paeonia rockii) was notable for the abundant unsaturated fatty acids in its seed oil. However, the role of WRI1 during the accumulation of P. rockii seeds oil remains largely unknown. RESULTS: In this study, a new member of the WRI1 family was isolated from P. rockii and was named PrWRI1. The ORF of PrWRI1 consisted of 1269 nucleotides, encoding a putative protein of 422 amino acids, and was highly expressed in immature seeds. Subcellular localization analysis in onion inner epidermal cells showed that PrWRI1 was located at the nucleolus. Ectopic overexpression of PrWRI1 could significantly increase the total fatty acid content in Nicotiana benthamiana leaf tissue and even PUFAs in transgenic Arabidopsis thaliana seeds. Furthermore, the transcript levels of most genes related to fatty acids (FA) synthesis and triacylglycerol (TAG) assembly were also up-regulated in transgenic Arabidopsis seeds. CONCLUSIONS: Together, PrWRI1 could push carbon flow to FA biosynthesis and further enhance the TAG amount in seeds with a high proportion of PUFAs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04127-9. |
format | Online Article Text |
id | pubmed-9990299 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-99902992023-03-08 Tree peony transcription factor PrWRI1 enhances seed oil accumulation Xie, Lihang Hu, Jiayuan Yan, Zhenguo Li, Xinyao Wei, Sailong Xu, Ruilin Yang, Weizong Gu, Huihui Zhang, Qingyu BMC Plant Biol Research BACKGROUND: WRINKLED1 (WRI1) encodes a transcription factor, belonging to the APETALA2 (AP2) family, and plays a key role in regulating plant oil biosynthesis. As a newly woody oil crop, tree peony (Paeonia rockii) was notable for the abundant unsaturated fatty acids in its seed oil. However, the role of WRI1 during the accumulation of P. rockii seeds oil remains largely unknown. RESULTS: In this study, a new member of the WRI1 family was isolated from P. rockii and was named PrWRI1. The ORF of PrWRI1 consisted of 1269 nucleotides, encoding a putative protein of 422 amino acids, and was highly expressed in immature seeds. Subcellular localization analysis in onion inner epidermal cells showed that PrWRI1 was located at the nucleolus. Ectopic overexpression of PrWRI1 could significantly increase the total fatty acid content in Nicotiana benthamiana leaf tissue and even PUFAs in transgenic Arabidopsis thaliana seeds. Furthermore, the transcript levels of most genes related to fatty acids (FA) synthesis and triacylglycerol (TAG) assembly were also up-regulated in transgenic Arabidopsis seeds. CONCLUSIONS: Together, PrWRI1 could push carbon flow to FA biosynthesis and further enhance the TAG amount in seeds with a high proportion of PUFAs. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04127-9. BioMed Central 2023-03-07 /pmc/articles/PMC9990299/ /pubmed/36882682 http://dx.doi.org/10.1186/s12870-023-04127-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Xie, Lihang Hu, Jiayuan Yan, Zhenguo Li, Xinyao Wei, Sailong Xu, Ruilin Yang, Weizong Gu, Huihui Zhang, Qingyu Tree peony transcription factor PrWRI1 enhances seed oil accumulation |
title | Tree peony transcription factor PrWRI1 enhances seed oil accumulation |
title_full | Tree peony transcription factor PrWRI1 enhances seed oil accumulation |
title_fullStr | Tree peony transcription factor PrWRI1 enhances seed oil accumulation |
title_full_unstemmed | Tree peony transcription factor PrWRI1 enhances seed oil accumulation |
title_short | Tree peony transcription factor PrWRI1 enhances seed oil accumulation |
title_sort | tree peony transcription factor prwri1 enhances seed oil accumulation |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9990299/ https://www.ncbi.nlm.nih.gov/pubmed/36882682 http://dx.doi.org/10.1186/s12870-023-04127-9 |
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