Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus

BACKGROUND: Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. METHODS: A specific region of DNA sequences from the...

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Detalles Bibliográficos
Autores principales: Fernandes, Blenda, Santrer, Emanoelle Fernandes Rutren La, de Figueiredo, Sônia Maria, Rocha-Silva, Fabiana, Assunção, Cláudia Barbosa, Abreu, Amanda Gabrielle, Santiago, Iara Furtado, Johann, Susana, Caligiorne, Rachel Basques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9991107/
https://www.ncbi.nlm.nih.gov/pubmed/36888783
http://dx.doi.org/10.1590/0037-8682-0217-2022
Descripción
Sumario:BACKGROUND: Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. METHODS: A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique. RESULTS: Three specific primers with 100% specificity for the Sporothrix genus were generated. CONCLUSIONS: PCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis.

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