Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus

BACKGROUND: Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. METHODS: A specific region of DNA sequences from the...

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Autores principales: Fernandes, Blenda, Santrer, Emanoelle Fernandes Rutren La, de Figueiredo, Sônia Maria, Rocha-Silva, Fabiana, Assunção, Cláudia Barbosa, Abreu, Amanda Gabrielle, Santiago, Iara Furtado, Johann, Susana, Caligiorne, Rachel Basques
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Medicina Tropical - SBMT 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9991107/
https://www.ncbi.nlm.nih.gov/pubmed/36888783
http://dx.doi.org/10.1590/0037-8682-0217-2022
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author Fernandes, Blenda
Santrer, Emanoelle Fernandes Rutren La
de Figueiredo, Sônia Maria
Rocha-Silva, Fabiana
Assunção, Cláudia Barbosa
Abreu, Amanda Gabrielle
Santiago, Iara Furtado
Johann, Susana
Caligiorne, Rachel Basques
author_facet Fernandes, Blenda
Santrer, Emanoelle Fernandes Rutren La
de Figueiredo, Sônia Maria
Rocha-Silva, Fabiana
Assunção, Cláudia Barbosa
Abreu, Amanda Gabrielle
Santiago, Iara Furtado
Johann, Susana
Caligiorne, Rachel Basques
author_sort Fernandes, Blenda
collection PubMed
description BACKGROUND: Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. METHODS: A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique. RESULTS: Three specific primers with 100% specificity for the Sporothrix genus were generated. CONCLUSIONS: PCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis.
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spelling pubmed-99911072023-03-08 Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus Fernandes, Blenda Santrer, Emanoelle Fernandes Rutren La de Figueiredo, Sônia Maria Rocha-Silva, Fabiana Assunção, Cláudia Barbosa Abreu, Amanda Gabrielle Santiago, Iara Furtado Johann, Susana Caligiorne, Rachel Basques Rev Soc Bras Med Trop Short Communication BACKGROUND: Sporotrichosis, a cosmopolitan mycosis caused by dimorphic fungi of the Sporothrix complex, affects humans and animals. This study aimed to develop new molecular markers for Sporothrix genome detection in biological samples using PCR. METHODS: A specific region of DNA sequences from the Sporothrix genus, publicly available in GenBank, was chosen for primer design. After testing the in silico specificity of these primers, in vitro specificity was evaluated using the PCR technique. RESULTS: Three specific primers with 100% specificity for the Sporothrix genus were generated. CONCLUSIONS: PCR using the designed primers can be used to develop molecular diagnostics for sporotrichosis. Sociedade Brasileira de Medicina Tropical - SBMT 2023-03-06 /pmc/articles/PMC9991107/ /pubmed/36888783 http://dx.doi.org/10.1590/0037-8682-0217-2022 Text en https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License
spellingShingle Short Communication
Fernandes, Blenda
Santrer, Emanoelle Fernandes Rutren La
de Figueiredo, Sônia Maria
Rocha-Silva, Fabiana
Assunção, Cláudia Barbosa
Abreu, Amanda Gabrielle
Santiago, Iara Furtado
Johann, Susana
Caligiorne, Rachel Basques
Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus
title Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus
title_full Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus
title_fullStr Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus
title_full_unstemmed Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus
title_short Sporotrichosis: In silico design of new molecular markers for the Sporothrix genus
title_sort sporotrichosis: in silico design of new molecular markers for the sporothrix genus
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9991107/
https://www.ncbi.nlm.nih.gov/pubmed/36888783
http://dx.doi.org/10.1590/0037-8682-0217-2022
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