Cargando…

A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models

Adequate pain management is essential for ethical and scientific reasons in animal experiments and should completely cover the period of expected pain without the need for frequent re-application. However, current depot formulations of Buprenorphine are only available in the USA and have limited dur...

Descripción completa

Detalles Bibliográficos
Autores principales: Wolter, Angelique, Bucher, Christian H., Kurmies, Sebastian, Schreiner, Viktoria, Konietschke, Frank, Hohlbaum, Katharina, Klopfleisch, Robert, Löhning, Max, Thöne-Reineke, Christa, Buttgereit, Frank, Huwyler, Jörg, Jirkof, Paulin, Rapp, Anna E., Lang, Annemarie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9992384/
https://www.ncbi.nlm.nih.gov/pubmed/36882427
http://dx.doi.org/10.1038/s41598-023-30641-9
_version_ 1784902299895726080
author Wolter, Angelique
Bucher, Christian H.
Kurmies, Sebastian
Schreiner, Viktoria
Konietschke, Frank
Hohlbaum, Katharina
Klopfleisch, Robert
Löhning, Max
Thöne-Reineke, Christa
Buttgereit, Frank
Huwyler, Jörg
Jirkof, Paulin
Rapp, Anna E.
Lang, Annemarie
author_facet Wolter, Angelique
Bucher, Christian H.
Kurmies, Sebastian
Schreiner, Viktoria
Konietschke, Frank
Hohlbaum, Katharina
Klopfleisch, Robert
Löhning, Max
Thöne-Reineke, Christa
Buttgereit, Frank
Huwyler, Jörg
Jirkof, Paulin
Rapp, Anna E.
Lang, Annemarie
author_sort Wolter, Angelique
collection PubMed
description Adequate pain management is essential for ethical and scientific reasons in animal experiments and should completely cover the period of expected pain without the need for frequent re-application. However, current depot formulations of Buprenorphine are only available in the USA and have limited duration of action. Recently, a new microparticulate Buprenorphine formulation (BUP-Depot) for sustained release has been developed as a potential future alternative to standard formulations available in Europe. Pharmacokinetics indicate a possible effectiveness for about 72 h. Here, we investigated whether the administration of the BUP-Depot ensures continuous and sufficient analgesia in two mouse fracture models (femoral osteotomy) and could, therefore, serve as a potent alternative to the application of Tramadol via the drinking water. Both protocols were examined for analgesic effectiveness, side effects on experimental readout, and effects on fracture healing outcomes in male and female C57BL/6N mice. The BUP-Depot provided effective analgesia for 72 h, comparable to the effectiveness of Tramadol in the drinking water. Fracture healing outcome was not different between analgesic regimes. The availability of a Buprenorphine depot formulation for rodents in Europe would be a beneficial addition for extended pain relief in mice, thereby increasing animal welfare.
format Online
Article
Text
id pubmed-9992384
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-99923842023-03-09 A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models Wolter, Angelique Bucher, Christian H. Kurmies, Sebastian Schreiner, Viktoria Konietschke, Frank Hohlbaum, Katharina Klopfleisch, Robert Löhning, Max Thöne-Reineke, Christa Buttgereit, Frank Huwyler, Jörg Jirkof, Paulin Rapp, Anna E. Lang, Annemarie Sci Rep Article Adequate pain management is essential for ethical and scientific reasons in animal experiments and should completely cover the period of expected pain without the need for frequent re-application. However, current depot formulations of Buprenorphine are only available in the USA and have limited duration of action. Recently, a new microparticulate Buprenorphine formulation (BUP-Depot) for sustained release has been developed as a potential future alternative to standard formulations available in Europe. Pharmacokinetics indicate a possible effectiveness for about 72 h. Here, we investigated whether the administration of the BUP-Depot ensures continuous and sufficient analgesia in two mouse fracture models (femoral osteotomy) and could, therefore, serve as a potent alternative to the application of Tramadol via the drinking water. Both protocols were examined for analgesic effectiveness, side effects on experimental readout, and effects on fracture healing outcomes in male and female C57BL/6N mice. The BUP-Depot provided effective analgesia for 72 h, comparable to the effectiveness of Tramadol in the drinking water. Fracture healing outcome was not different between analgesic regimes. The availability of a Buprenorphine depot formulation for rodents in Europe would be a beneficial addition for extended pain relief in mice, thereby increasing animal welfare. Nature Publishing Group UK 2023-03-07 /pmc/articles/PMC9992384/ /pubmed/36882427 http://dx.doi.org/10.1038/s41598-023-30641-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Wolter, Angelique
Bucher, Christian H.
Kurmies, Sebastian
Schreiner, Viktoria
Konietschke, Frank
Hohlbaum, Katharina
Klopfleisch, Robert
Löhning, Max
Thöne-Reineke, Christa
Buttgereit, Frank
Huwyler, Jörg
Jirkof, Paulin
Rapp, Anna E.
Lang, Annemarie
A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models
title A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models
title_full A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models
title_fullStr A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models
title_full_unstemmed A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models
title_short A buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models
title_sort buprenorphine depot formulation provides effective sustained post-surgical analgesia for 72 h in mouse femoral fracture models
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9992384/
https://www.ncbi.nlm.nih.gov/pubmed/36882427
http://dx.doi.org/10.1038/s41598-023-30641-9
work_keys_str_mv AT wolterangelique abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT bucherchristianh abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT kurmiessebastian abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT schreinerviktoria abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT konietschkefrank abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT hohlbaumkatharina abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT klopfleischrobert abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT lohningmax abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT thonereinekechrista abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT buttgereitfrank abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT huwylerjorg abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT jirkofpaulin abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT rappannae abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT langannemarie abuprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT wolterangelique buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT bucherchristianh buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT kurmiessebastian buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT schreinerviktoria buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT konietschkefrank buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT hohlbaumkatharina buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT klopfleischrobert buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT lohningmax buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT thonereinekechrista buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT buttgereitfrank buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT huwylerjorg buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT jirkofpaulin buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT rappannae buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels
AT langannemarie buprenorphinedepotformulationprovideseffectivesustainedpostsurgicalanalgesiafor72hinmousefemoralfracturemodels